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Diss Factsheets
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EC number: 418-370-0 | CAS number: 143925-92-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-05-24 to 1996-06-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 92/69/EEC
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted April 4, 1984
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations tested
- Sampling method:
During the test duplicate samples were taken from the control (0 mg/L) and the saturated test substance solution (100%-filtrate) and one dilution (25%-fiItrate) for analysis on the two major compounds.
Sampling:
Frequency: at t= 0 h and at t= 48 h.
Volume: 10 ml from the approximate centre of the test vessel.
- Sample storage conditions before analysis: stored in deep-freeze - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
The test substance is a mixture of eight compounds, which are all hardly soluble in water (< 0.0005 g/L). Initially, test solutions were prepared at 100 mg/L using Tween 80 as a dispersent. These solutions were stirred for ca. 68 hours. Subsequently, the water phase of one solution was separated from the undissolved
fraction of the test substance by filtration through a paper filter (Schleicher and Schuell 604, ca. 5 µm). The filtrate was tested together with the unfiltered solution.
The test solutions used in the test originated from those prepared for the fish toxicity test (please refer to section 6.1.1). In addition to the filtrate prepared at 100 mg/L (100%), dilutions of the filtrate were prepared containing 25% and 50% of the filtrate. Also, a non filtered supersaturated solution of 100 mg/L was included in the test.
- Differential loading: 100 mg/L
- Evidence of undissolved material: In the unfiltered dispersion, the major part of the undissolved test substance was observed floating at the surface. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Straus, 1820
- Age at study initiation: For the test selection of young daphnia with an age of < 24 hours.
- Method of breeding:
Start of each batch: With newborn animals, i.e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel.
Maximum age of the cultures: 4 weeks
Renewal of the cultures: After 7 days of cultivation half of the medium twice a week.
- Feeding during test: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L expressed as CaCO3
- Test temperature:
- 20.4 to 20.6 °C
- pH:
- 7.4 to 8.0
- Dissolved oxygen:
- 8.9 to 9.4 mg O2/L
- Nominal and measured concentrations:
- Nominal:
The final test was a limit study exposing daphnia to a supersaturated solution of 100 mg/L and the water phase of a supersaturated solution of 100 mg/L (100%) and two dilutions containing 25% and 50% of the filtrate.
Measured:
The actual concentrations were based on measurement of two compounds with molecular masses (M) of 510.7 and 522.6. The concentration of both components were just above the maximum detection level of 0.4 mg/L in the samples taken from 100 % filtrate at the start of the test, i.e. 0.54 mg/L of the compound with M= 522.6 and 0.61 mg/L of the compound with M= 510.7. The recoveries found in the samples taken at the end of the test showed that the concentration of the compound with M= 522.6 had decreased by 20%, whereas the concentration of the compound with M= 510.7 had decreased by 30%.
Hence, these results showed that it was not possible to prepare solutions which contained test concentrations at levels largely exceeding 0.5 mg/L (maximum solubility) even when starting from a supersaturated solution with a nominal concentration of 100 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL, all glass
- Aeration: No aeration of the test solution.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 10 organisms per vessel containing 80 ml medium
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to EU and OECD guidelines
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED:
Immobility (including mortality): At 24 hours and 48 hours.
pH and oxygen: At the beginning and at the end of the test, for all concentrations and the control.
Temperature of medium: Daily in one control vessel, beginning at the start of the test.
TEST CONCENTRATIONS
- Range finding study:
Ten daphnids per vessel were exposed to either the water phase of a test substance solution prepared at a concentration of 100 mg/L or the unfiltered dispersion at nominal 100 mg/L. Both test solutions contained Tween 80 at 100 mg/L. Also a negative control and an additive control were tested. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: none
- Other biological observations: none
- Immobilisation of control: 0 organisms
- Other adverse effects control: none
- Abnormal responses: none
- Any observations that might cause a difference between measured and nominal values: Substance deposits were observed in the vessels containing the unfiltered solution of 100 mg/L - Results with reference substance (positive control):
- - Results with reference substance valid: yes
The 48h-EC50 was 0.65 mg/lLwith 95 % confidencial limits of 0.56 - 0.83 mg/L.
Reference
Description of key information
A GLP guideline study according to OECD 202 was conducted (NOTOX 166837, 1996). Due to the low water solubility of this UVCB, two test solutions were prepared by stirring 100 mg/L (nominal concentration) for ca. 68 hours. The water phase of one solution was separated from the undissolved fraction of the test substance by filtration through a paper filter while the second solution was tested unfiltered. In addition, dilutions of the filtrate were prepared containing 25% and 50% of the filtrate.
Due to this procedure it can be assumed that the daphnids were exposed to the maximal concentration possible in the medium used.
No significant effects concerning mobility and toxicity were observable in all test solutions after a test duration of 48 hours. Therefore, the tested UVCB is considered to be acutely not harmful to daphnids in the range of its water solubility.
Key value for chemical safety assessment
Additional information
With high probability acutely not harmful to daphnids, no toxic effects occur in the range of water solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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