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Diss Factsheets
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EC number: 442-450-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 Apr - 13 May 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Remarks:
- standard activated sludge
- Details on inoculum:
- - Source of inoculum/activated sludge: standard activated sludge purchased from Chemicals Inspection & Testing Institute, Japan, on 22 Jan 1998 (Sludge No.100122)
- Method of cultivation: 24.9 - 25.1 °C, > 5 mg/L dissolved oxygen and pH 6.41 - 7.75
- Concentration of sludge: 3694 ppm
- Water filtered: no - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 24.9 - 25.1 °C
- pH: 7.1 - 8.19
- Suspended solids concentration: 30 mg/L, corresponding to 10^7 - 10^8 cells/L according to OECD 301
TEST SYSTEM
- Culturing apparatus: 300 mL
- Number of culture flasks/concentration: 3
- Measuring equipment: Coulometer (TypeOM-2001A)
SAMPLING
- Sampling frequency: oxygen consumption: measured automatically and continuously for 28 days by coulometer, residual quantity of the test substance: determined by liquid chromatography after pre-treatment of the test solution at the end of exposure
CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Abiotic sterile control: 1
- Toxicity control: - - Reference substance:
- aniline
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: -9% degradation
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: -2% degradation
- Results with reference substance:
- After 7 days: 62%
After 28 days: 72%
(Reported in the test report. The rates were based on ThOD_NO2)
Degradation rate:
After 7 days: 77%
After 14 days: 80%
(Re-evaluated from the test report. The rates are based on ThOD_NH3, which is approved by OECD 301) - Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
Reference
Table 1: Degradation rate based on oxygen consumption:
Bottle no |
Test bottle |
Amount of test substance added (mg) |
TOD (mg) |
7th day |
28th day |
||
BOD (mg) |
Degradation rate (%) |
BOD (mg) |
Degradation rate (%) |
||||
1 |
Procedure control |
30.3 |
91.1 |
60.3 |
62 |
75.9 |
72 |
2 |
Control blank |
- |
- |
4.0 |
- |
10.0 |
- |
3 |
Test suspension |
30.1 |
82.3 |
1.4 |
-3 |
3.6 |
-8 |
4 |
Test suspension |
30.0 |
82.1 |
1.5 |
-3 |
3.8 |
-8 |
5 |
Test suspension |
30.3 |
82.9 |
0.0 |
-5 |
1.5 |
-10 |
6 |
Abiotic control |
30.1 |
- |
0.0 |
- |
0.0 |
- |
|
|
|
|
Average degradation rate of test suspensions: -4 |
Average degradation rate of test suspensions: -9 |
||
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Table 2: Degradation rate of the test substance based on determination of residual quantity by liquid chromatography
Bottle no |
Test bottle |
Amount of test substance added (mg) |
Residual amount of test substance |
Degradation rate (%) |
|
3 |
Test suspension |
30.1 |
30.5 |
-3 |
|
4 |
Test suspension |
30.0 |
30.5 |
-3 |
|
5 |
Test suspension |
30.3 |
29.6 |
0 |
|
6 |
Abiotic control |
30.1 |
29.5 |
- |
|
|
|
|
|
Average degradation rate of test suspensions: -4 |
|
|
|
|
|
Table 3: Validity criteria for OECD 301 C
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
< 20% |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
Toxicity control not required |
Not applicable |
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg O2/L in 28 days. Values higher than 60 mg/L require critical examination of the data and the experimental technique. If the pH value is outside the range 6-8.5 and the oxygen consumption by the test substance is less than 60%, the test could be repeated with a lower concentration of test substance. |
BOD after 28 days in 300 mL = 10 mg, corresponding to 33.3 mg/L |
Yes |
A test is considered valid if the difference of extremes of replicate values of the removal of the test substance at the plateau or at the end of the test, as appropriate is less than 20% and if the percentage degradation of aniline calculated from the oxygen consumption exceeds 40% after 7 days and 65% after 14 days. |
BODr of reference compound by day 7: 60.3 mg BODr of reference compound by day 14: 64.8 mg BODp of procedure control by day 7: 4.0 mg BODp of procedure control by day 14: 6.2 mg Degradation = (BODr – BODp)/TOD ×100 Degradation rate: After 7 days: 77% After 14 days: 80% (Re-evaluated from the test report. The rates are based on ThOD_NH3 [TOD: 72.9 mg], which is approved by OECD TG 301) |
yes |
Description of key information
Not readily biodegradable: 0% biodegradation after 28 d (OECD 301 C)
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
One study is available assessing the biodegradability of the test item by activated sludge microorganisms. The test was performed according to OECD 301C and was conducted under GLP standards. 100 mg/L initial test concentration was mixed into 300 mL of an aqueous mineral test medium and inoculated with 30 mg/L sludge. The mixture was incubated for 28 d and the biodegradation of the test material was calculated from the oxygen consumption throughout the study and calculated from direct determination (LC).
- 9% degradation was determined from the oxygen consumption measurement. The residual rate calculated from direct determination of the test item was 100% in the test suspension at test termination. The test results indicate that the substance is not readily biodegradable.
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