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EC number: 909-044-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Test organisms (species):
- Daphnia magna
- Executive summary:
The 48-hour NOEC was at least 1000 mg test item/L (nominal) and the 48‑hour LOEC as well as the 48-hour EC50 were > 1000 mg test item/L (nominal) .
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP - guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- cited as Directive 92/69/EEC, C.2; in most parts equivalent to the OECD TG 202
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - samples were taken at the start (0 hrs) and end (48 hrs) of the experiment
- samples from control and test concentrations were analysed - Vehicle:
- no
- Details on test solutions:
- - stock solution: 120 mg of test substance were added to 1 litre of dilution water (M4 medium) and treated for 30 min. on a magnetic stirrer
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: STRAUS
- parthenogenetic females
- Source: Bundesgesundheitsamt, Berlin
- Age at study initiation (mean and range, SD): 0 - 24 hrs (neonates)
- feeding during test: no
METHOD OF BREEDING AND ACCLIMATISATION:
A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility under constant temperature conditions (20 +/ 1 °C) at a 16 : 8 light-dark photoperiod (illumination < 1000 lux). The culture water ('M4 medium') is partly renewed once a week. The daphnia are exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent daphnia during the culture period are recorded daily in a semi-quantitaive way. The neonates are separated from their parent daphnia by filtration prior the acute test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 264.2 mg/l CaCO3 (measured at test start)
- Test temperature:
- during test: 18-22°C ± 1°C
measured at the end of the test (48 h): 21.1-21.4°C - pH:
- measured at the end of the test (48 h): 7.2-7.9
- Dissolved oxygen:
- measured at the end of the test (48 h):
=> 7.9-8.4 mg/l
=> 89-96 % saturation - Nominal and measured concentrations:
- => 5 test concentrations (nominal): 6.25, 12.5, 25, 50 and 100 mg/l
=> 1 control - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml glass beakers with 20 ml of test medium
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Aeration: none
TEST MEDIUM
- M4 medium (originally described in Water Research 24 (9): 1157-1167)
- M4 medium was used for both, the breeding of the test animals and the preparation of stock and test solutions
OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- mobility - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 41.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 32.7-53.0
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 24.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 20.8-29.6
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- - probit analysis
- Validity criteria fulfilled:
- yes
- Executive summary:
In a GLP study according to Directive 92/69/EEC, C.2, with analytical monitoring (DOC and HPLC), the EC50 of Daphnia magna was 24.8 mg/l (nominal) after 48 h (Bayer AG, 2003). Due to rapid hydrolysis of DMP to MMP, and the slow hydrolysis of MMP to phosphonic acid, there were significant concentrations of MMP at any DMP concentration (22 - 35 % w/w of initial DMP). At the nominal DMP concentration of 25 mg/l, the geometric mean of the measured MMP concentrations was 8.0 mg/l during this test. DOC measurements confirmed the presence of virtually all organic carbon of the initial carbon content of DMP.
Referenceopen allclose all
Immobilisation for Daphnia magna (initial population: 20) - raw data:
|
control |
6.25 mg/l |
12.5 mg/l |
25 mg/l |
50 mg/l |
100 mg/l |
||||||
|
0 h |
48 h |
0 h |
48 h |
0 h |
48 h |
0 h |
48 h |
0 h |
48 h |
0 h |
48 h |
Absolute |
0 |
0 |
0 |
0 |
0 |
0 |
7 |
6 |
12 |
6 |
17 |
3 |
Cumulative |
0 |
0 |
0 |
0 |
0 |
0 |
7 |
13 |
12 |
18 |
17 |
20 |
Cumulative [%] |
0 |
0 |
0 |
0 |
0 |
0 |
35 |
65 |
60 |
90 |
85 |
100 |
Chemical analysis:
The results of the two different analytical methods (HPLC and DOC-determination) clearly demonstrate that Dimethylphosphit hydrolysed within a few hours and that the overall concentration of organic carbon (measured by DOC) was stable throughout the complete exposure period. Therefore the results were expected based on nominal concentrations.
|
control |
6.25 mg/l |
12.5 mg/l |
25 mg/l |
50 mg/l |
100 mg/l |
||||||
|
0 h |
48 h |
0 h |
48 h |
0 h |
48 h |
0 h |
48 h |
0 h |
48 h |
0 h |
48 h |
DOC replicates [mg/l] |
<5 /<5 |
<5 /<5 |
<5/<5 |
<5/<5 |
<5/<5 |
<5/<5 |
7 / 6 |
6 / 6 |
13 / 12 |
10 / 11 |
23 / 22 |
20 / 20 |
DOC mean value [mg/l] |
< 5 |
< 5 |
< 5 |
< 5 |
< 5 |
< 5 |
6.5 |
6 |
12.5 |
10.5 |
22.5 |
20 |
HPLC replicates Dimethylphosphit [mg/l] |
<0.375 |
<0.375 |
<0.375 |
<0.375 |
1.64 |
<0.375 |
5.10 |
<0.375 |
10.25 |
<0.375 |
48.11 |
<0.375 |
HPLC replicates Monomethylphosphit [mg/l] |
< 0.375 |
< 0.375 |
2.20* |
1.83* |
4.32* |
3.90* |
8.18* |
7.89* |
15.27* |
14.49* |
22.22* |
26.22* |
* Values calculated as dimethylphosphit.
Description of key information
For phosphonic ester residue, there is no short term toxicity study on invertebrates available. However, for two components, dimethyl phosphonate (CAS 868-85-9, DMP) and phosphonic acid (13598-36-2, PA) such studies are available. In the study with DMP, DMP was rapidly hydrolysed to monomethyl phosphonate (CAS 13590-71-1, MMP), which slowly hydrolyed to PA. Thus, it is plausible to assume that the study with DMP considers also potential effects of MMP, the third component of the registered substance.
In the study with DMP, the EC50 of Daphnia magna was 24.8 mg/L (nominal) after 48 h.
In the study with PA, the 48-hour NOEC was at least 1000 mg/L (nominal).
As worst case approach, the lowest 48 h-NOEC of 24.8 mg/L (nominal) is considered for phosphonic ester residue.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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