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Diss Factsheets
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EC number: 700-501-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-08-26 to 2009-04-15 (3rd draft report)
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction product of Acid Yellow 42, Acid Black 2 and Basic Violet 1
- IUPAC Name:
- Reaction product of Acid Yellow 42, Acid Black 2 and Basic Violet 1
- Reference substance name:
- Acid Yellow 42: 6375-55-9
- IUPAC Name:
- Acid Yellow 42: 6375-55-9
- Reference substance name:
- Acid Black 2: reaction product of 8005-03-6 and 68510-98-5
- IUPAC Name:
- Acid Black 2: reaction product of 8005-03-6 and 68510-98-5
- Reference substance name:
- Basic Violet 1: 8004-87-3
- IUPAC Name:
- Basic Violet 1: 8004-87-3
- Details on test material:
- - Name of test material: Reaction product of Acid Yellow 42, Acid Black 2 and Basic Violet 1
- Lot/batch No.: 2008041541
- Expiration date of the lot/batch: 2010-01-31
- Stability under test conditions: see expiry date
- Storage condition of test material: store cool and dry
- Analytical purity: 100 % (96.8 %; UVCB substance)
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge, microorganisms from a
domestic waste water treatment plant.
- Laboratory culture:
- Method of cultivation:
- Storage conditions:
- Storage length:
- Preparation of inoculum for exposure:
- Pretreatment:
- Concentration of sludge: 4 g/L
- Initial cell/biomass concentration:
- Water filtered: yes
- Type and size of filter used, if any: cotton wool - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 3 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Reconstituted water: In deionised water analytical grade salts were added:
a) 4.25 g KH2PO4, 10.875 g K2HPO4, 33.59 g Na2HPO4 x 12H2O, 0.25 g NH4Cl filled up with deionised water to 500 mL volume#
b) 11.25 g MgSO4 x 7H2O filled up with deionised water to 500 mL volume
c) 18.20 g CaCl2 x 2H2O filled up with deionised water to 500 mL volume
d) 0.25 g FeCl3 x 6H2O filled up with deionised water to 1000 mL volume (stock solution was applied on the day of preparation)
- Test temperature: 19.6 - 20.9 °C
- pH: 7.35
- pH adjusted: no
- Aeration of dilution water: 8.8 mg O2/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: BOD flasks
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment: large glass tanks with valve, large glass bottles with valve, narrow necked, 300 ml bod bottles with glass stoppers, funnels and coarse filter papers, moisture content analyzer, centrifuge, balance, self stirring o2 electrode, oxygen and ph meter, aeration system, incubator with thermometer
- Test performed in closed vessels due to significant volatility of test substance:
CONTROL AND BLANK SYSTEM
- Inoculum blank: Only filtered inoculum was added to 6.80 litres of aqueous test medium.
- Toxicity control: 20.8 mg of F 46 Black and reference item stock solution* (69.0 mL) were mixed into 6.90 litres of aqueous test medium corresponding to 3 mg/L test item (COD of 5.76 mg O2/L) and 3.6 mg/L reference item (ThODNH4 of 6.012 mg O2/L).
* the concentration of the stock solution was 360 mg/L
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 3.6 mg/L
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0.9
- Sampling time:
- 28 d
- Remarks on result:
- other: mean value, based on the measured COD
BOD5 / COD results
BOD5 / COD
- Parameter:
- COD
- Value:
- 7.55 mg O2/g test mat.
- Results with reference substance:
- The reference item Sodium benzoate was sufficiently degraded to a mean of 87.5 % after 14 days, and to a mean of 96.7 % after 28 days of incubation, based on ThODNH4, thus confirming the suitability of the used activated sludge inoculum.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Under the test conditions the percentage biodegradation of F 46 Black reached a mean of 0.9 % after 28 days based on the measured COD of the test item. The test item was not ready biodegradable (level for ready biodegradability is removal of 60 % COD).
- Executive summary:
The test item was considered not ready biodegradable.
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