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Diss Factsheets
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EC number: 203-581-0 | CAS number: 108-42-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: scientifically acceptable and sufficient documented
Data source
Reference
- Reference Type:
- publication
- Title:
- The detection of carcinogen-induced sperm head abnormalities in mice
- Author:
- Topham JC
- Year:
- 1 980
- Bibliographic source:
- Mutat Res 69, 149-155 (1980)
Materials and methods
- Principles of method if other than guideline:
- Groups of 5 (male CBA x female BALB/c) F1 male mice; 10-12 weeks old; received 5 daily i.p. injections of the test substance (25, 50, 100 or 200 mg/kg bw) or vehicle alone (5 ml/kg bw/day). Positive controls (20 mg/kg/day cyclophosphamide monohydrate) are included in the study. 5 weeks after the last dose the mice are killed and sprem from the cauda epididymidis are suspended in saline. Smears of sperm from each animal are dried and stained with Eosin Y. Sperm heads are classified as of normal or abnormal morphology. differences in the incidence of abnormal sprem between the groups are compared using the Wilcoxson Rank Sum Test.
- GLP compliance:
- no
- Type of method:
- in vivo
Test material
- Reference substance name:
- 3-chloroaniline
- EC Number:
- 203-581-0
- EC Name:
- 3-chloroaniline
- Cas Number:
- 108-42-9
- Molecular formula:
- C6H6ClN
- IUPAC Name:
- 3-chloroaniline
- Test material form:
- other: liquid
- Details on test material:
- m-chloroaniline was obtained from Aldrich Chemical Co. (Gillingham, England)
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: (male CBA x female BALB/c) F1 male mice
- Sex:
- male
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- not specified
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 5 days
- Frequency of treatment:
- daily
- Duration of test:
- 5 days treatment and 5 weeks post exposure period
Doses / concentrations
- Remarks:
- Doses / Concentrations:
25, 50, 100 or 200 mg/kg bw
Basis:
- No. of animals per sex per dose:
- 5 animals per dose
- Control animals:
- yes, concurrent vehicle
Results and discussion
Effect levels
- Dose descriptor:
- NOAEL
- Effect level:
- 200 mg/kg bw/day
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- other: sperm head abnormalities
Any other information on results incl. tables
m-chloroaniline did not significantly increase the incidence of abnormal sperm heads.
Applicant's summary and conclusion
- Executive summary:
Groups of 5 (male CBA x female BALB/c) F1 male mice; 10-12 weeks old; received 5 daily i.p. injections of the test substance (25, 50, 100 or 200 mg/kg bw) or vehicle alone (5 ml/kg bw/day). Positive controls (20 mg/kg/day cyclophosphamide monohydrate) are included in the study. 5 weeks after the last dose the mice are killed and sprem from the cauda epididymidis are suspended in saline. Smears of sperm from each animal are dried and stained with Eosin Y. Sperm heads are classified as of normal or abnormal morphology. Differences in the incidence of abnormal sprem between the groups are compared using the Wilcoxson Rank Sum Test.
As a result, m-chloroaniline did not significantly increase the incidence of abnormal sperm heads in the highest applied dose of 200 mg/kg bw (1/2 LD50).
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