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Diss Factsheets
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EC number: 700-737-4 | CAS number: 1220100-43-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study carried out according to OECD Guideline 202
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- The protocol stated culture water will be maintained @20±2 ºC. Two weeks prior to testing the temp of the culture water ranged from 20-23ºC. This temp range was well within the typical temp encountered in the natural environment so no negative impact.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N,N'-bis[2-hydroxy-3-(2,2,3,3- tetrafluoropropoxy)propyl]-N,N,N',N'-tetramethylethane-1,2- diaminium dichloride
- IUPAC Name:
- N,N'-bis[2-hydroxy-3-(2,2,3,3- tetrafluoropropoxy)propyl]-N,N,N',N'-tetramethylethane-1,2- diaminium dichloride
- Reference substance name:
- 1,1,2,2,17,17,18,18-octafluoro-6,13-dihydroxy-8,8,11,11-tetramethyl-4,15-dioxa-8,11-diazaoctadecane-8,11-diium dichloride
- EC Number:
- 700-737-4
- Cas Number:
- 1220100-43-5
- Molecular formula:
- C18H34F8N2O4.2Cl
- IUPAC Name:
- 1,1,2,2,17,17,18,18-octafluoro-6,13-dihydroxy-8,8,11,11-tetramethyl-4,15-dioxa-8,11-diazaoctadecane-8,11-diium dichloride
- Details on test material:
- [14C] Radiolabelled test substance was received and stored frozen in the original container.
Non Radiolabelled test substance was received and stored at room temperature in the original container in a dessicator.
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At test initiation (0 hour) and test termination (48 hours), triplicate samples were removed from each test solution and the control and samples were analyzed for [14C] residues by liquid scintillation counting (LSC). The highest test solution was also analyzed for distribution of radioactivity by high performance liquid chromatography with radiochemical detection (HPLC/RAM), at each interval. At test initiation, test solution samples were taken from the intermediate vessels prior to division into replicate vessels. At test termination, the replicate solutions from each respective concentration and the control were composited prior to sampling.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnids were selected as the test organism since they are recommended by U.S. EPA and OECD and are commonly used in acute freshwater invertebrate toxicity tests. The Daphnia magna used in this toxicity test were obtained from laboratory cultures maintained at Smithers Viscient.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Total hardness as CaCO3: 190 mg/L
- Test temperature:
- 20 to 21 ºC
- pH:
- pH: 8.0 to 8.1
- Dissolved oxygen:
- 8.5 to 8.6 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations of 4.3, 9.4, 21, 45 and 100 mg/L were selected for the definitive exposure.
Measured concentrations of 4.1, 9.3, 21, 44 and 100 mg/L were defined by analytical monitoring. - Details on test conditions:
- The culture water was prepared by fortifying well water based on the formula for hard water (U.S. EPA, 1975) and filtering it through an Amberlite XAD 7 resin column to remove any potential organic contaminants. For the two weeks prior to testing, this water had a total hardness and a total alkalinity range as calcium carbonate (CaCO3) of 190 mg/L and 96 to 100 mg/L, respectively, and a specific conductivity range of 720 to 850 micromhos per centimeter (µmhos/cm). A pH range of 7.7 to 8.3, a temperature range of 20 to 23 ºC, and a dissolved oxygen concentration range of 6.3 to 9.6 mg/L were measured in the culture solutions during the two weeks prior to test initiation.
The daphnid culture area received a regulated photoperiod of 16 hours of light and 8 hours of darkness. Light at an intensity of 69 to 88 footcandles at the surface of the culture solutions was provided by fluorescent bulbs. Daphnids were fed unicellular green algae, Ankistrodesmus falcatus (4 x 107 cells/mL), in addition to a suspension of YCT (yeast, cereal leaves and flaked fish food). In culture, daphnids were fed various amounts based on their age. Daphnids that were 0 to 6 days old were fed 1.0 mL of algae and 0.5 mL of YCT suspension per vessel per day. Daphnids that were 7 to 10 days old were fed 1.5 mL of algae and 0.5 mL of YCT suspension per vessel per day. Daphnids that were > 10 days old were fed 2.0 mL of algae and 0.5 mL of YCT suspension per vessel per day. Representative samples of the food source were analyzed periodically for the presence of pesticides, PCBs and toxic metals by GeoLabs, Inc., Braintree, Massachusetts. None of these compounds have been detected in the food samples at concentrations considered toxic in any of the samples analyzed, in agreement with ASTM (2002) standard practice. Based on the analysis for pesticides, PCBs and toxic metals, the food sources were considered to be of acceptable quality since all analyte concentrations were below limits of concern. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 33 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 29 to 39 mg/L @ 95 % CL (EC50 value was determined by Trimmed Spearman-Karber)
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 24 to 36 mg/L @ 95 % CL (EC50 value was determined by Trimmed Spearman-Karber)
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 9.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- EC50 value was determined by Trimmed Spearman-Karber.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on mean measured concentrations, the 48-hour EC50 value for Daphnia magna exposed to the test substance was determined by Trimmed Spearman-Kärber to be 30 mg/L with 95% confidence intervals of 24 to 36 mg/L. The highest concentration producing 0% immobilization was 9.3 mg/L and the lowest concentration producing 100% immobilization was > 100 mg/L, the highest concentration tested. The No-Observed-Effect concentration (NOEC) was determined to be 9.3 mg/L.
- Executive summary:
The EC50 and NOEC of the test substance to daphnids (Daphnia magna) under static test conditions has been determined in accordance with the OECD guideline 202 under GLP conditions.
The 48 -hour EC50 value for Daphnia magna exposed to the test substance was determined by Trimmed Spearman-Kärber to be 30 mg/L.
The No-Observed-Effect concentration (NOEC) was determined to be 9.3 mg/L.
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