Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 431-770-1 | CAS number: 216698-07-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02.06. - 18.07.1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline study, GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted July 21, 1997
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- NOTOX B.V., Hambakenwetering 7, 5231 DD ‘s-Hertogenbosch, The Netherlands
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- -
- EC Number:
- 431-770-1
- EC Name:
- -
- Cas Number:
- 216698-07-6
- Molecular formula:
- C32 H44 O4
- IUPAC Name:
- 2-[2-oxo-5-(2,4,4-trimethylpentan-2-yl)-2,3-dihydro-1-benzofuran-3-yl]-4-(2,4,4-trimethylpentan-2-yl)phenyl acetate
- Details on test material:
- - Description: White solid
- Purity: 98.7%
- Storage condition of test material: At room temperature in the dark
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix from rat liver prepared five days after i.p. treatment of male adult Wistar rats with 500 mg/kg bw Aroclor 1254 (20% solution in corn oil; w/v).
- Test concentrations with justification for top dose:
- Doses:
Preliminary toxicity test in TA100 and WP2UvrA strain: 0, 3, 10, 33, 100, 333, 1000, 3330, 5000 µg/plate tested in the absence and presence of S9-mix. Solvent was 0.1 mL DMSO. 3 plates per dose and control.
Main test (1st and 2nd experiment):
standard plate test 0, 10, 33, 100, 333, 1000 µg/plate tested in the absence and presence of S9-mix. The first mutation experiment was performed with the strains TA1535, TA1537 and TA98 and the second mutation experiment was performed with the strains TA1535, TA1537, TA98, TA100 and WP2UvrA. Solvent was 0.1 mL DMSO. 3 plates per dose and control. - Vehicle / solvent:
- DMSO (0.1 mL)
- Justification for choice of solvent/vehicle: The test substance was insoluble in water but soluble in DMSO.
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Without S9-mix: Sodium azide; 9-aminoacridine; daunomycine; methylmethanesulfonate, 4-nitroquinoline N-oxide. With S9-mix: 2-aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
NUMBER OF REPLICATIONS: three
DETERMINATION OF CYTOTOXICITY
- Method: colony growth - Evaluation criteria:
- No formal hypothesis testing was done.
A test substance is considered negative (not mutagenic) in the test if:
a) The total number of revertants in any tester strain at any concentration is not greater than two times the solvent control value, with or without metabolic activation.
b) The negative response should be reproducible in at least one independently repeated experiment.
A test substance is considered positive (mutagenic) in the test if:
a) It induces at least a 2-fold, dose related increase in the number of revertants with respect to the number induced by the solvent control in any of the tester strains, either with or without metabolic activation.
However, any mean plate count of less than 20 is considered to be not significant.
b) The positive response should be reproducible in at least one independently repeated experiment.
The preceding criteria were not absolute and other modifying factors might enter into the final evaluation decision. - Statistics:
- Mean and standard deviation from 3 plates/concentration were calculated.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES:
The test substance was tested in the tester strains TA100 and WP2 uvrA with concentrations of 3, 10, 33, 100, 333, 1000, 3330 and 5000 µg/plate in the absence and presence of S9-mix. This dose range finding test is reported as a part of the first experiment of the mutation test. The test substance precipitated in the top agar at concentrations of 333 µg/plate and upwards. Precipitation of test substance on the plates was observed at the start and at the end of the incubation period at concentrations of 1000 µg/plate and upwards in tester strain TA100 and WP2 uvrA. To determine the toxicity of the test substance, the reduction of the bacterial background lawn, the increase in the size of the microcolonies and the reduction of the revertant colonies were observed. No reduction of the bacterial background lawn and no decrease in the number of revertants were observed.
MAIN TEST
Based on the results of the dose range finding study, the test substance was tested up to concentrations of 1000 µg/plate in the absence and presence of S9-mix in two mutation experiments. The first mutation experiment was performed with the strains TA1535, TA 1537 and TA98 and the second mutation experiment was performed with the strains TA1535, TA1537, TA98, TA100 and WP2UvrA. Precipitation occurred in the top agar at concentrations of 333 and 1000 µg/plate. Precipitation of the test substance on the plates was observed at the start and at the end of the incubation period at the concentration of 1000 µg/plate in all tester strains. The bacterial background lawn was not reduced at all concentrations tested and no decrease in the number of revertants was observed. All bacterial strains showed negative responses over the entire dose range, i.e. no dose-related, two-fold, increase in the number of revertants in two independently repeated experiments. The negative and strain-specific positive control values were within our laboratory background historical control data ranges indicating that the test conditions were adequate and that the metabolic activation system functioned properly. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Results of the range finder and Experiment I
TA 1535 | TA 1537 | TA 98 | TA 100 | WP2uvrA | ||||||
Dose (µg/plate) | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 |
0 | 8 | 11 | 4 | 4 | 17 | 17 | 70 | 98 | 8 | 9 |
3 | 90 | 72 | 9 | 10 | ||||||
10 | 11 | 11 | 4 | 3 | 15 | 20 | 74 | 94 | 11 | 14 |
33 | 9 | 8 | 4 | 4 | 1 | 21 | 79 | 91 | 8 | 10 |
100 | 9 | 10 | 3 | 3 | 15 | 21 | 77 | 94 | 7 | 10 |
333 | 14 | 7 | 2 | 3 | 12 | 20 | 79 | 76 | 5 | 11 |
1000SP | 9 | 10 | 3 | 4 | 15 | 17 | 82 | 78 | 10 | 14 |
3330SP | 85 | 81 | 9 | 8 | ||||||
5000MP | 86 | 73 | 6 | 12 | ||||||
positive control* | 310 | 568 | 253 | 426 | 441 | 872 | 907 | 1372 | 901 | 118 |
Results of Experiment II
TA 1535 | TA 100 | TA 1537 | TA 98 | WP2uvrA | ||||||
Dose (µg/plate) | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 |
0 | 9 | 9 | 5 | 4 | 16 | 23 | 100 | 98 | 12 | 11 |
10 | 12 | 12 | 5 | 4 | 15 | 28 | 91 | 90 | 10 | 12 |
33 | 10 | 11 | 9 | 5 | 16 | 24 | 98 | 106 | 8 | 9 |
100 | 12 | 7 | 8 | 6 | 15 | 21 | 108 | 95 | 10 | 10 |
333 | 8 | 13 | 5 | 5 | 16 | 21 | 89 | 86 | 11 | 11 |
1000SP | 10 | 10 | 6 | 3 | 15 | 25 | 105 | 99 | 11 | 7 |
positive control* | 366 | 484 | 243 | 471 | 562 | 853 | 908 | 1130 | 536 | 373 |
SP: slight precipitate
MP: moderate precipitate
*Positive Controls
without metabolic activation:
sodium azide: 1 µg/plate (TA 1535)
9-aminoacridine: 60 µg/plate (TA 1537)
daunomycine: 4 µg/plate (TA 98)
methylmethanesulfonate: 650 µg/plate (TA 100)
4-nitroquinoline N-oxide: 10 µg/plate (WP2uvrA)
with metabolic activation:
2-aminoanthracene: 2.5µg/plate (TA 1537, TA 1535, TA 98), 1 µg/plate (TA100), 5 µg/plate (WP2uvrA)
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Based on the results of this study it is concluded that the test substance is not mutagenic in the Salmonella typhimurium reverse mutation assay and in the Escherichia coli reverse mutation assay. - Executive summary:
The test substance was tested in the Salmonella typhimurium reverse mutation assay with four histidine-requiring strains of Salmonella typhimurium (TA1535, TA1537, TA100 and TA98) and in the Escherichia coli reverse mutation assay with a tryptophan-requiring strain of Escherichia coli WP2UvrA in two independent experiments. These tests were following GLP guidelines and were based on the OECD testing guideline 471. In the dose range finding test, the test article was tested up to concentrations of 5000 µg/plate in the absence and presence of S9-mix in the strains TA100 and WP2UvrA. At this dose level no toxicity was observed. Precipitation occurred on the plates at dose levels of 1000 µg/plate and above. In the mutation assays, the test substance was tested up to concentrations of 1000 µg/plate in the absence and presence of S9-mix. The test article precipitated on the plates at this dose level. The bacterial background lawn was not reduced at all concentrations tested and no decrease in the number of revertants was observed. No dose-related increase in the number of revertant both in the absence and presence of S9-metabolic activation was observed. These results were confirmed in an independently repeated experiment. Based on the results of this study it is concluded that the test article is not mutagenic in the Salmonella typhimurium reverse mutation assay and in the Escherichia coli reverse mutation assay.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.