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EC number: 214-406-2 | CAS number: 1125-21-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Equivalent to OECD 423; GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Qualifier:
- according to guideline
- Guideline:
- other: ACUTE TOXIC CLASS METHOD (ATC METHOD) BY E. SCHLEDE, U. MISCHKE, R. ROLL, D. KAYSER: A NATIONAL VALIDATION STUDY OF THE ACUTE-TOXIC-CLASS METHOD AN ALTERNATIVE TO THE LD50 TEST. ARCH. TOXICOL. 66: 455-470 (1992).
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- 2,6,6-trimethylcyclohex-2-ene-1,4-dione
- EC Number:
- 214-406-2
- EC Name:
- 2,6,6-trimethylcyclohex-2-ene-1,4-dione
- Cas Number:
- 1125-21-9
- Molecular formula:
- C9H12O2
- IUPAC Name:
- 2,6,6-trimethylcyclohex-2-ene-1,4-dione
- Reference substance name:
- 2,6,6-Trimethyl-2-cyclohexen-1,4-dion
- IUPAC Name:
- 2,6,6-Trimethyl-2-cyclohexen-1,4-dion
- Reference substance name:
- Oxoisophoron
- IUPAC Name:
- Oxoisophoron
- Details on test material:
- - NAME OF TEST SUBSTANCE: OXOISOPHORON
- CHEMICAL NAME: 2,6,6-TRIMETHYL-2-CYCLOHEXEN-1,4-DION
- SUBSTANCE NUMBER: 96/87
- BATCH NUMBER: NOT KNOWN
- CAS NUMBER: 1125-21-9
- DEGREE OF PURITY/CONTENT: 98.87 A%
- DATE OF MANUFACTURING: 18-MAY-1995
- PHYSICAL STATE/APPEARANCE: LIQUID, YELLOW
- STORAGE CONDITIONS: ROOM TEMPERATURE
Constituent 1
Constituent 2
Constituent 3
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Dr. K. Thomae GmbH, Biberach, FRG
- Age at study initiation: young adult animals
- Weight at study initiation: 150-300 g
- Fasting period before study: 16 h
- Housing: single
- Diet (e.g. ad libitum): Kliba-Labordiaet 343, Klingenthalmühle AG, Kaiseraugst, Switzerland, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period: at least 1 week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12 h/12 h
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 40 g/100 mL
- Amount of vehicle (if gavage): 5 mL/kg bw
- Justification for choice of vehicle: The test substance could not be homogenized in water.
MAXIMUM DOSE VOLUME APPLIED: 5 mL/kg bw
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: Based on the physical characteristics of the test substance and the composition no pronounced acute oral toxicity was expected. - Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 3
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Weighing was performed shortly before administration, weekly thereafter and at the end of the study (before fasting period). Recording of signs and symptoms several times on the day of administration, at least once each workday for th individual animals.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, pathology
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- No mortality observed.
- Clinical signs:
- Males
Signs observed in all males were impaired general state, poor general state, dyspnoea, apathy, staggering and piloerection. These signs had disappeared within few hours or the first two days.
Additional signs observed in 2 of 3 male animals were reported as abdominal position, lateral position, ataxia, atonia and paresis. These signs had disappeared within few hours or the first two days.
Females
Signs observed in all female animals were poor general state, dyspnoea, apathy, ataxia, atonia, paresis and exsiccosis. These signs had disappeared within few hours or the first two days.
Additional signs observed in 2 of 3 females were impaired general state, abdominal position and staggering. These signs had disappeared within few hours or the first two days.
Signs of lateral position and twitching had only been observed in 1 female rat at the very beginning of the observation period. - Body weight:
- Mean body weight of male rats [g]:
before application/day 6/ day 13: 189/248/283
Mean body weight of male rats [g]:
before application/day 6/ day 13: 176/200/214 - Gross pathology:
- no pathologic findings noted.
Applicant's summary and conclusion
- Conclusions:
- Based on the results of this study, the test substance 2,6,6-trimethylcyclohex-2-ene-1,4-dione does not need to be classified according to EU Directive 67/548 and Regulation (EU) No. 1272/2008.
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