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EC number: 700-584-3 | CAS number: 1217271-02-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: The (controlled) activated sludge was supplied by the sewage plant for domestic sewage in Balatonfüred, Hungary on 15 June 2011.
- Preparation of inoculum for exposure: The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in isotonic saline solution and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 5 g dry material per litre was mixed with reconstituted water (see above) and then aerated until use (in this study 100 mL inoculum was prepared). Before use the sludge was filtered through cotton wool. The pH of the activated sludge inoculum was checked after preparation and before use. The pH of the activated sludge inoculum after preparation was 6.91. pH adjustment before use was not performed.
- Concentration of sludge: 5 g dw/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to the guideline
- Test temperature: 22 ± 2 °C
- pH: prior study 7.26
- pH adjusted: yes
- Aeration of dilution water: aeration of dilution water until use
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Winkler bottles with glass stoppers
- Number of culture flasks/concentration:
10 (+2 reserve) bottles containing the test item and inoculum
10 (+2 reserve) bottles containing the reference item and inoculum (procedure control)
10 (+2 reserve) bottles containing only inoculum (inoculum control)
10 (+2 reserve) bottles containing the test item, reference item and inoculum (toxicity control)
- Method used to create aerobic conditions: aeration system
- Measuring equipment: Complex measuring system with O2 electrode and pH electrode and built in temperature probe Aeration system, Incubator with thermometer, Balance, Centrifuge, Moisture analyzer.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Only filtered inoculum was added to the aqueous test medium.
- Toxicity control: Adequate amount of Sika Hardener MH and reference item stock solution was mixed into the aqueous test medium corresponding to the investigated 2.0 mg/L test item concentration [chosen based on the theoretical oxygen demand (ThODNO3 of 6.0 mg O2/L) of the test item] and to 3.6 mg/L concentration of the reference item (ThODNH4 of 6.012 mg O2/L).
- Procedure Control: Based on the theoretical oxygen demand (ThODNH4) of Sodium benzoate (1.67 mg O2 per mg) (details on calculation are given in the guidelines), at the start of the test a stock solution of Sodium benzoate was mixed into the aqueous test medium (corresponding to 3.6 mg/L reference item, respectively a ThODNH4 of about 6.012 mg O2/L). - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- The purpose of this study was to determine the ready biodegradability of the test item Sika Hardener MI. The test item was exposed to activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Under the test conditions the percentage biodegradation of Sika Hardener MI reached a mean of -0.7 % (that was taken into consideration as 0 %) after 28 days based on ThODNO3. (The highest percentage biodegradation value, 3.7 % of Sika Hardener MI was observed on the 21st day based on ThODNO3.) Therefore, the test item can be considered to be not ready biodegradable.
- Key result
- Parameter:
- BOD5
- Value:
- <= 0 mg O2/g test mat.
- Results with reference substance:
- The reference item Sodium benzoate was sufficiently degraded to a mean of 60.5 % after 14 days, and to a mean of 76.3 % after 28 days of incubation, based on ThODNH4. The percentage biodegradation of the reference item confirms the suitability of the used activated sludge inoculum.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test item can be considered to be not ready biodegradable. According to the test guidelines the pass level for ready biodegradability is removal of 60 % ThODNO3.
- Executive summary:
The purpose of this study was to determine the ready biodegradability of the test item Sika Hardener MI. The test item was exposed to activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. Under the test conditions the percentage biodegradation of Sika Hardener MI reached a mean of -0.7 % (that was taken into consideration as 0 %) after 28 days based on ThODNO3. (The highest percentage biodegradation value, 3.7 % of Sika Hardener MI was observed on the 21st day based on ThODNO3.) Therefore the test item can be considered to be not ready biodegradable. The reference item Sodium benzoate was sufficiently degraded to a mean of 60.5 % after 14 days (69.2 % degradation was observed already on the 7th day of the test), and to a mean of 76.3 % after 28 days of incubation, based on ThODNH4, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the reference item, a mean of 30.1 % biodegradation was noted within 14 days and 30.4 % biodegradation after 28 days of incubation. (The highest biodegradation value of 31.2 % was observed on the 21st day of the test.) Thus, the test item can be assumed to not inhibit the activated sludge microorganisms (higher than 25 % degradation occurred within 14 days).
Reference
Percentage Biodegradation at Different Time Intervals during the Exposure Period of 28 Days
Treatment |
Concentration |
Flask |
Percent of biodegradation after n days of exposure |
|||
[mg/L] |
No. |
7 |
14 |
21 |
28 |
|
Test item |
|
1a |
-0.7 |
-4.5 |
4.9 |
-1.2 |
2.0 |
1b |
-1.4 |
-4.0 |
2.6 |
-0.2 |
|
|
mean |
-1.1 |
-4.2 |
3.7 |
-0.7 |
|
Reference item |
|
2a |
63.1 |
59.8 |
63.3 |
76.6 |
3.6 |
2b |
75.3 |
61.2 |
65.4 |
75.9 |
|
|
mean |
69.2 |
60.5 |
64.3 |
76.3 |
|
Toxicity control |
Test item: 2.0 |
4a |
30.9 |
30.4 |
31.0 |
31.2 |
4b |
30.3 |
29.9 |
31.3 |
29.6 |
||
mean |
30.6 |
30.1 |
31.2 |
30.4 |
Description of key information
The ready biodegradability of the test item was assessed over a 28 day period in a closed bottle test (EU method C 4-E). The test material attained up to 0 % biodegradation after 28 days whereas the reference substance (Sodium benzoate) was 60.5 % degraded 28 days. These results indicate that the test item cannot be classified readily biodegradable under conditions of the test.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Closed bottle test:
The purpose of this study was to determine the ready biodegradability of the test item Sika Hardener MI. The test item was exposed to activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. Under the test conditions the percentage biodegradation of Sika Hardener MI reached a mean of -0.7 % (that was taken into consideration as 0 %) after 28 days based on ThODNO3. (The highest percentage biodegradation value, 3.7 % of Sika Hardener MI was observed on the 21st day based on ThODNO3.) Therefore the test item can be considered to be not ready biodegradable. The reference item Sodium benzoate was sufficiently degraded to a mean of 60.5 % after 14 days (69.2 % degradation was observed already on the 7th day of the test), and to a mean of 76.3 % after 28 days of incubation, based on ThODNH4, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the reference item, a mean of 30.1 % biodegradation was noted within 14 days and 30.4 % biodegradation after 28 days of incubation. (The highest biodegradation value of 31.2 % was observed on the 21st day of the test.) Thus, the test item can be assumed to not inhibit the activated sludge microorganisms (higher than 25 % degradation occurred within 14 days).
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