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EC number: 212-084-8 | CAS number: 760-93-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Rationale for reliability incl. deficiencies:
- other: EPA guideline similar to OECD-guideline.
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
Methacrylic acid is the hydrolysis product of Methacrylic anhydride. One molecule of methacrylic anhydride willl hydrolyse into two molelules methacrylic acid.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
Source substance: Methacrylic acid, purity >= 98 %
Target substance: Methacrylic anhydride: purity: >=94 %, other components: Methacrylic acid and adducts of methacrylic aanhydride and adducts of methacrylic acid and methacrylic anhydride.
3. ANALOGUE APPROACH JUSTIFICATION
Like other acid anhydrides, methacrylic anhydride was found to be hydrolytically unstable in water at pH 4, 7 and 9. The half lives (DT50) were found to be 56.4 min at pH 4 (25 °C), 25.5 min at pH 7 (25 °C) respectively 10.3 min at 37 °C and 2.29 min at pH 9. The initial hydrolysis product is methacrylic acid (Noack 2012).
4. Data Matrix
Target: Methacrylic anhydride CAS 760-93-0
Source: Methacrylic acid CAS 79-41-4 - Reason / purpose for cross-reference:
- read-across source
- Analytical monitoring:
- yes
- Details on sampling:
- At each sampling period 5 ml aliquots of test water were collected from each of the four replicates of each test solution. Two replicates at each time
point were composited. - Vehicle:
- no
- Details on test solutions:
- A primary stock standard of MAA was prepared and stored at room temperature. Subsequent dilutions were prepared in Milipore water for use as
spiking solutions and test dilution water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- ABC Laboratories in house culture. The primary culture was obtained from the Columbia National Fisheries Research Laboratory (CNFRL), Columbia, Missouri, in 1977
Age at study initiation: first instar (<=24 hours old).
Organisms were held in temperature controlled area at 20°C.
Lighting was 50-70 footcandles on a 16 hr photoperiod, with 30 minute dawn and dusk transition periods.
During the holding period, the daphnids were fed a suspension of algae (Selenastrum capricornutum) supplemented with Tetramin®/cereal leaves/yeast suspension.
Organisms were not fed during the 48 hr treatment period. - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 172-178 mg/L
- Test temperature:
- 20°C
- pH:
- 7.0-7.9
- Dissolved oxygen:
- 7.7-8.2 mg/L
- Nominal and measured concentrations:
- Nominal = 7.2, 14, 30, 60 and 120 mg/L.
Mean measured (sampling at 0 and 48 hrs) = 6.7, 13, 32, 62 and 130 mg/L. - Details on test conditions:
- Method: flow-through bioassay as described in American Public Health Association and Standard Methods for the Examination of Water and
Wastewater.
A half liter proportional diluter system was used for intermittent introduction of dilution water and MAA into the test chambers. The test system contained six sets of four replicate one liter test chambers designated as control, and level 1 through level 5. The proportional diluter was calibrated preior to use in the test.
Dilution water was a blend of reverse osmosis water and ABC laboratory well water.
10 organisms were assigned to each of the four replicate test chambers (40 Daphnids per concentration).
Water quality measurements were recorded at 0 and 48 hours. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 130 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: based on lack of immobilization and or abnormal effects
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 130 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: The 48 hr dose response slope could not be determined because of the lack of an effect level
- Details on results:
- Overall, the measured concentrations represent 101-7.4% of nominal concentrations. Quality control samples analyzed with each sample day over a range of 6.00 to 150 mg/L with recoveries averaging 94+/-19%. Low recovery for the 0 hour sampling was obtained. Duplicate low spikes at teh 48 hour sampling were analyzed to verify the recovery of MAA at the 6.00 mg/l concentration.
Measurement at 0 and 48 hrs of the MAA diluter stock solution averaged 12,000 mg/L which represented 100% of the nominal concentration (12,000 mg/L). - Validity criteria fulfilled:
- yes
- Conclusions:
- Methacrylic anhydride rapidly hydrolysis to methacrylic acid in aqueous media. Therefore assessment of aquatic toxicity to Methacrylic anhydride based on studies with methacrylic acid is recommended.
In a valid guideline study, the 48 hour EC50 for Daphnia magna was >130 mg/L Methacrylic acid. - Executive summary:
Methacrylic anhydride rapidly hydrolysis to methacrylic acid in aqueous media. Therefore assessment of aquatic toxicity to Methacrylic anhydride based on studies with methacrylic acid is recommended.
Methacrylic acid was tested in an acute test to aquatic invertebrates acc. EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity test Test) with Daphnia magna. The definitive flow-through acute toxicity of Methacrylic Acid to Daphnia magna was conducted the following nominal concentrations: 7.2, 14, 30, 60 and 120 mg/1.
Forty first-instar daphnids were exposed to each of the test concentrations and control.
The mean measured concentrations calculated from analytical sampling at 0 and 48 hours are as follows: 6.7, 13, 32, 62 and 130 mg/1. The toxicity of Methacrylic Acid based on mean measured test
concentrations were EC50 (48h): > 130 mg/l, NOEC (48h): 130 mg/l
Water quality parameters of temperature, dissolved oxygen and pH were measured in all test concentrations at 0- and 48-hours and were determined to be within acceptable limits. The overall ranges for these
parameters were: daily diluter temperature observations = 20°C, pH = 7.0 - 7.9 and dissolved oxygen= 7.7 - 8.2 mg/1.
Reference
Description of key information
EC50 (48 h) of the hydrolysis product methacrylic acid for freshwater invertebrates: > 130 mg/l
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 130 mg/L
Additional information
Methacrylic anhydride is hydrolytically unstable in water. The initial hydrolysis product is methacrylic acid. The EC50 (48 h) of methacrylic acid for fresh water invertebrates was > 130 mg/l (measured concentration). Methacrylic acid was also tested with marine invertebrates with EC50 (48 h) of 210 mg/l (nominal;concentration analytically confirmed, 0-8 % differing from nominal concentrations under test conditions).
The following information is taken into account for any hazard / risk / persistency assessment:
EC50 (48 h) of the hydrolysis product methacrylic acid for fresh water invertebrates: > 130 mg/l
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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