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EC number: 236-655-6 | CAS number: 13455-36-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from October 22, 1998 to February 11, 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- version February 24, 1987
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1100 (Acute Oral Toxicity)
- Version / remarks:
- version August 1998
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- Tricobalt bis(orthophosphate)
- EC Number:
- 236-655-6
- EC Name:
- Tricobalt bis(orthophosphate)
- Cas Number:
- 13455-36-2
- Molecular formula:
- Co3(PO4)2
- IUPAC Name:
- tricobalt bis(orthophosphate)
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Remarks:
- BR
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Young adult albino rats of the CrL:CD(SB)BR strain
- Source: Charle River Laboratories,Inc., Protage, Michigan on October 19 and 26 and November 9, 1998
- Acclimatisation period: At least 7 days
- Housing: group caging by sex
- Cage type: Suspended, stainless steel cages
- Diet: Continuous access to Rodent Diet #8604 (Harlan Teklad) and water except for 17 to 20 hours before test material administration when the diet, not water, was withheld. The diet is routinely analized by the manufacturer for nutritional components and environmental contaminants. Samples of water are periodically analyzed. There were no known contaminants in the diet or water at levels that would be expected to interfere with or affect the results of the study.
- Sex: Male and female healthy
- Age of animals at dosing: Approximately 8 to 12 weeks of age
- Body weight range at dosing: 201-269 g
- Identificaiton: by animal number and corresponding ear tag throughout the study
ENVIRONMENTAL CONDITIONS
- Lighting period: 12 hours light/12-hour dark cycle
- Temperature: 18 - 26°C
- Relative humidity: 30 - 70 %
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Remarks:
- distilled water
- Details on oral exposure:
- The test material was mixed with distilled water to a specific concentration for each dose level. Each prepared test material mixture appeared to be a suspension. An individual dose of each respective test materail mixutre was calculated for each animal based on its fasted body weight and administered as a single gavage dose at a volume of 5mL/kg of body weight. The test material mixtures were stored at room temperature until adminsitered.
- Doses:
- 1000, 1500 and 2000 mg/kg
- No. of animals per sex per dose:
- 5 females and 5 males per dose
- Control animals:
- no
- Details on study design:
- Initially, a single dose of 1000 mg/lg was administered to five males and five females. Based on the results of this initial dose level, additonal dose levels were added at the direction of the study director in oder to meet the objectives of the study: 1500 mg/kg, then 2000 mg/kg.
Clinical observations were conducted at 1, 2.5 and 4 hours after test material administration and daily thereafter for 14 days. Mortality checks were conducted twice a day (morning and afternoon) for 133 days after test material administration and again the morning of Day 14.
Body weights were determined before test material adminsitration (Day 0). Additional body weights ware determined Day 7, at termination of the respective in-life phase (Day 14), or at death when survival exceeded the day of treatment.
At termination of the respective in-life phase for each dose level, surviving animals were euthanized. All animals, whether found dead during the study or euthanized, were subjected to a macroscopic necropsy examinaiton and any abnormalities were recorded. After necropsy, the animals were discarded and no tissues saved. - Statistics:
- Usinf the mortalities values observed during the study, the estimated LD50 values for females and the sexes combined were determined by a computer program using modified Behrens-Reed-Muench cumulant method. No other statistical evaluations were required by the protocol.
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 851 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 538 - <= 2 228
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 1 651 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 210 - <= 2 252
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- One female animal treated at 1000 mg/kg ws found dead within 3 days of test material administration. Two female animals treated at 1500 mg/kg were found dead within 4 days of test material administration. Two males and three females treated at 2000 mg/kg were found dead within 4 days of test material administration.
- Clinical signs:
- other: other: red stained face, black or liquid faces, drak stained urogenital area, thin appearance, hunched posture, death.
- Gross pathology:
- At necropsy, most changes observed were in the animals that died and the more prominent observations pertained to the content of the gastrointestinal tract. In most of these animals given 1500 or 2000 mg/kg, the stomach and intestines were observed to contain dark fluid or semisolid material described variably being black or tan and purple. This content possibly represented test material mixed with ingesta. Some of these animals also had black material (staining) in the haircoat of the perianal or perineal regions, or the haircoat of the entire ventral surface. All remaining observations were considered incidental findings and unrelated to the test material.
Any other information on results incl. tables
CLINICAL SIGNS DESCRIPTION:
Clinical signs of toxicity were observed in one male and three femals treated at 1000 mg/kg and included red stained face, black or liquid feces, dark stained urogenital area, thin appearance and death. The surviving animals treated at this dose level returned to a normal appearance by Day 7 after treatment.
All animal treated at 1500 mg/kg appeared normal troughout the study with the exception of two females which were found dead on Day 4.
Clinical signs of toxicity observed at the 2000 mg/kg dose level included red stained face, liquid black feces, thin appearance, hunched posture and death. The surviving animals treated at this dose level returned to a normal appearance by Day 6 after treatment.
BODY WEIGHTS:
Animals surviving till the end of the observation period exhibited body weight gain during the study with the exception of one female at 1000 mg/kg which exhibited an insignificant wieght loss of 9g during the second week.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Based on the mortality observed in the study conducted in accordance with O.E.C.D. test guideline n° 401, the estimated oral LD50 values of the test item in rats were determined to be:
- greater than 2000 mg/kg for males,
- 1651 mg/kg for females, and
- 1851 mg/kg for the sexes combined.
According to the criteria for classification, packaging and labelling of dangerous substances and preparations in accordance with the Regulation EC n°1272/2008, the test item has to be classified in category 4. The signal word 'Warning' and hazard statement H302 'Harmful if swallowed' are required.
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