Sodium polysulfide aluminosilicate with a SOD-type framework structure

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

OECD guideline 202. GLP study.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

The Notice on the Test Method Concerning New Chemical Substances < Algal Growth Inhibition Test, Daphnia Acute Immobilization Test, and Fish Acute Toxicity Test > (final revision: April 1, 2005)

GLP compliance:

yes

Specific details on test material used for the study:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Solubility and stability of the test substance in the test medium: Since the test substance is a poorly water-soluble inorganic compound, the test was carried out using the test item in the dispersed state. As a result of analysis, the ratio of the measured value to the nominal value was 93% at the time of preparation of the test solution and 1% after 24 hours. It is considered that the concentration of the middle layer after 24 hours decreased due to the gradual precipitation of the test substance in the test solution. As a reference, the filtrate obtained by filtering the test solution with a filter (Millipore Millex HA 0.45 μm) was similarly analyzed, and the concentration of the test substance dissolved in the test solution was confirmed. As a result of analysis, the average value of the test substance concentration in the filtrate was 0.146 mg/L.

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0 (control), 100 mg/L
- Sampling method: the concentration of test item was verified at 0 and 24 hours.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Since the test substance is a poorly water-soluble inorganic compound, the test was carrie d out using the test item in the dispersed state.

Test organisms (species):

Daphnia magna

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

20 ± 1ºC

Nominal and measured concentrations:

- 0 (control), 100 mg/L (nominal); 20.8 mg/L (measured, mean).
- The ratio of the measured value to the nominal value was 93% at the time of preparation of the test solution and 1% after 24 hours (precipitation).

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 100 mL / vessel
- Renewal rate of test solution (frequency/flow rate): 24h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h light / 8h dark
- Light intensity: indoor light (800 lux or less)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility (24, 48 h)

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 20.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 20.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 20.8 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality/Immobilisation of control: none reported
- Dissolved oxygen concentration: not specified
- Any observations that might cause a difference between measured and nominal values: precipitation

Validity criteria fulfilled:

yes

Conclusions:

The 48h-EC50 of the test item in daphnids was determined to be > 20.8 mg/L, based on measured concentration.

Executive summary:

An acute toxicity test was performed on Daphnia magna according to OECD 202, under GLP conditions. To determine the degree of inhibition in daphnids' mobility, a limit test was performed under semi-static conditions (renewal rate 24h). 20 daphnids per concentration (4 vessels with 5 animals each) were exposed to 0 (control) or 100 mg/L (nominal concentrations) of test item for 48h at 20 ± 1 ºC. The concentration of test item was verified at 0 and 24 hours by ICP-MS. Under test conditions, the 48h-EC50 of the test item in daphnids was determined to be > 20.8 mg/L, based on measured concentration.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

December 2004

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

The study is not well documented. No GLP study.

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Age at study initiation: less than 24 hours, coming from mothers with minimum 3 lays.
- Age of the mothers: 23 days

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test temperature:

20 ± 2ºC

pH:

7.67

Dissolved oxygen:

8.92 mg/l

Nominal and measured concentrations:

Measured concentrations: 50 - 75 - 90 % of test material

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Volume of solution:
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hrs light / 8 hrs dark
- Light intensity: 800 - 1200 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation (24, 48 h)

VEHICLE CONTROL PERFORMED: yes/no

RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

Potassium dicromate

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 90 other: %

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

50 other: %

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

not determinable

Results with reference substance (positive control):

- Results with reference substance valid? yes (EC50 between 0.6 - 2.1 mg/L; not performed in parallel with the definitive test)
- EC50 (24h) = 0.799 mg/L [0.674 - 0.897]

No data on nominal/measured and 95%CL

Validity criteria fulfilled:

yes

Remarks:

(immobilisation in the control < 10%; dissolved oxygen concentration > 2 mg/L; valid result with positive control)

Conclusions:

The result was as follows: EC50 (24h) > 90%
It is not observed any immediate toxic effect on the Daphnia magna. It is observed a decantation of the test material.

Executive summary:

The aim of this study was to investigate the degree of inhibition of the Daphnia magna´s mobility produced by the test material.

The test procedure used was the French norme NF ISO 6341 .

Concentrations used were: 50 -75 -90 % of test material

The result was as follows: EC50 (24h) > 90%

It is not observed any immediate toxic effect on the Daphnia magna. It is observed a decantation of the test material.

Description of key information

Key study. Method according to OECD 202, GLP study. The 48h-EC50 of the test item in Daphnia magna was determined to be > 20.8 mg/L (measured concentration).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

20.8 mg/L

Additional information

Supporting study. A study performed according to the French standard NF ISO 6341, reports a 24h-EC50 of the test item in Daphnia magna as 90% of the test item. Since no further data was provided on the purity or concentration of the test item, the information cannot be used for assessment.