Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SSPECIES
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: fingerling
COMMENT: 1.0 G, 4.6 CM
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
7.9 to 8.2
Dissolved oxygen:
Saturated mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 29000 NR TO NR 29000 ug/L NR
F 37000 NR TO NR 37000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s),48 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 29000 NR TO NR 29000 ug/L NR
F 37000 NR TO NR 37000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR 7.9 TO 8.2
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
29 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 5.8 mg/l (NOEC). One of the 96h-LC50 was 29 mg/l
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
37 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 7.4 mg/l (NOEC). One of the 96h-LC50 was 37 mg/l
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Under the conditions of the present test sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 5.6 mg/l (NOEC). One of the 48h-LC100 was 56 mg/l
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)
ENDPOINT

ENDPOINT: Lethal concentration to 100% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)
BCF Value ():
Sublethal observations / clinical signs:

Preliminary testing of eight collectors (xanthates) and four frothers in 96-h static and 28-day flow-through bioassays using rainbow trout as the test organism show a great disparity in the toxicity of the chemicals administered in these two ways.

For the short-term tests, the relative toxicity of the compounds is expressed as an lc50 or as a range of concentration in mg l-1 in which the lc50 is expected to fall. Of the collectors tested in this way sodium ethyl and potassium amyl xanthate were the most toxic, with lc50's in the range of 30-50 mg l-1. Among the frothers, xylenol (cresylic acid) was found to be the most toxic (5.6 mg l-1 >lc50 > 3.2 mg l-1) while polypropylene glycol was least toxic (lc50 > 1000 mg l-1).

The long-term tests using potassium ethyl, sodium isopropyl, sodium ethyl, and potassium amyl xanthate indicated that in the flow-through system, the toxicity of the chemicals was in the order of 100 fold greater compared with the static bioassay results.

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 5.8 mg/l (NOEC). One of the 96h-LC50 was 29mg/l
Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 7.4 mg/l (NOEC). One of the 96h-LC50 was 37 mg/l
Under the conditions of the present test sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 5.6 mg/l (NOEC). One of the 48h-LC100 was 56 mg/l
Executive summary:

Under the conditions of the present test Sodium ethyl xanthate  induced no mortality in Oncorhynchus mykiss  (Rainbow Trout) below 5.8 mg/l (NOEC). One of the 96h-LC50 was 29mg/l

Under the conditions of the present test Sodium ethyl xanthate  induced no mortality in Oncorhynchus mykiss  (Rainbow Trout) below 7.4 mg/l (NOEC). One of the 96h-LC50 was 37 mg/l

Under the conditions of the present test sodium ethyl xanthate  induced no mortality in Oncorhynchus mykiss  (Rainbow Trout) below 5.6 mg/l (NOEC). One of the 48h-LC100 was 56 mg/l

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: 0.9 G,7.3 G
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
NR
Dissolved oxygen:
NR
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 29000 NR TO NR 29000 ug/L NR
F 37000 NR TO NR 37000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s),48 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 29000 NR TO NR 29000 ug/L NR
F 37000 NR TO NR 37000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR NR TO NR
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: NR NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
29 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 5.8 mg/l (NOEC). One of the 96h-LC50 was 29 mg/l
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
37 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 7.4 mg/l (NOEC). One of the 96h-LC50 was 37 mg/l
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)
Sublethal observations / clinical signs:

Preliminary testing of eight collectors (xanthates) and four frothers in 96-h static and 28-day flow-through bioassays using rainbow trout as the test organism show a great disparity in the toxicity of the chemicals administered in these two ways.

For the short-term tests, the relative toxicity of the compounds is expressed as an lc50 or as a range of concentration in mg l-1 in which the lc50 is expected to fall. Of the collectors tested in this way sodium ethyl and potassium amyl xanthate were the most toxic, with lc50's in the range of 30-50 mg l-1. Among the frothers, xylenol (cresylic acid) was found to be the most toxic (5.6 mg l-1 >lc50 > 3.2 mg l-1) while polypropylene glycol was least toxic (lc50 > 1000 mg l-1).

The long-term tests using potassium ethyl, sodium isopropyl, sodium ethyl, and potassium amyl xanthate indicated that in the flow-through system, the toxicity of the chemicals was in the order of 100 fold greater compared with the static bioassay results.

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 5.8 mg/l (NOEC). One of the 96h-LC50 was 29 mg/l .
Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 7.4 mg/l (NOEC). One of the 96h-LC50 was 37 mg/l
Executive summary:

Under the conditions of the present test Sodium ethyl xanthate  induced no mortality in Oncorhynchus mykiss  (Rainbow Trout) below 5.8 mg/l (NOEC). One of the 96h-LC50 was 29 mg/l .

Under the conditions of the present test Sodium ethyl xanthate  induced no mortality in Oncorhynchus mykiss  (Rainbow Trout) below 7.4 mg/l (NOEC). One of the 96h-LC50 was 37 mg/l.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: fingerling
COMMENT: 1-10 G
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
348 mg/L CaCO3
ALKALINITY: 203 mg/L CaCO3
Test temperature:
12 0C
pH:
8.6
Dissolved oxygen:
Saturated mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F NR 14000 TO 16 000 14000 TO 16000 ug/L NR
F NR 13000 TO 15 000 13000 TO 15000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F NR 14000 TO 16 000 14000 TO 16000 ug/L NR
F NR 13000 TO 15 000 13000 TO 15000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 12 NR TO NR C
pH: 8.6 NR TO NR
HARDNESS: 348 NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: 203 NR TO NR mg/L CaCO3
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 13 - < 15 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 2.6 mg/l (NOEC). One of the 96h-LC50 was between 13 and 15mg/l
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 14 - < 16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 2.8 mg/l (NOEC). One of the 96h-LC50 was between 14 and 16 mg/l
Details on results:
Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 2.6 mg/l (NOEC). One of the 96h-LC50 was between 13 and 15mg/l .
Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 2.8 mg/l (NOEC). One of the 96h-LC50 was between 14 and 16 mg/l

EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)
GENERAL COMMENTS: HARD,ALK,PH/OF DILUTION H2O// AERATED//
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 2.6 mg/l (NOEC). One of the 96h-LC50 was between 13 and 15mg/l .
Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 2.8 mg/l (NOEC). One of the 96h-LC50 was between 14 and 16 mg/l
Executive summary:

Under the conditions of the present test Sodium ethyl xanthate  induced no mortality in Oncorhynchus mykiss  (Rainbow Trout) below 2.6 mg/l (NOEC). One of the 96h-LC50 was  between 13 and 15mg/l .

Under the conditions of the present test Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss  (Rainbow Trout) below 2.8 mg/l (NOEC). One of the 96h-LC50 was  between 14 and 16 mg/l

Endpoint:
short-term toxicity to fish
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.
Qualifier:
according to guideline
Guideline:
other: QSAR
Principles of method if other than guideline:
EPI Suite v 4.1/ECOSARv1.00
GLP compliance:
no
Remarks:
not applicable to QSAR models
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
other: Fishes species
Details on test organisms:
no further information
Test type:
other: QSAR model
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
not applicable. QSAR model
Test temperature:
not applicable. QSAR model
pH:
not applicable. QSAR model
Dissolved oxygen:
not applicable. QSAR model
Salinity:
not applicable. QSAR model
Details on test conditions:
100% Sodium ethyl xanthate (CAS No. 140-90-9)
SMILES : S=C([S-])OCC.[Na+]
CHEM : Carbonodithioic acid, o-ethyl ester, sodium salt
MOL FOR: C3 H5 O1 S2 Na1
MOL WT : 144.18
Log Kow: 0.87 (used)
Wat Sol: 1.79E+004 mg/L (WskowWin estimate)




Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
934.623 mg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
not specified
Details on results:
Using the EPIWIN ECOSAR QSAR model, a 96hr LC50 for freshwater fish of 934.623 mg/l was established.
Fish, 96-hr LC50 =934.623 mg/L (ppm)
Validity criteria fulfilled:
yes
Remarks:
US EPA accepted QSAR method for chemicals properties assessment.
Conclusions:
Using the EPIWIN ECOSAR QSAR model, a 96hr LC50 for freshwater fish of 943.623 mg/l was established.
Fish, 96-hr LC50 =934.623 mg/L (ppm)
Fish, 96-hr (SW) LC50 =1416.171 mg/L (ppm)
Fish 14-day LC50 = 927.914 mg /L (ppm)
Fish 30-day ChV = 90.256 mg/L (ppm)
Fish (SW) 30-day ChV = 70.993 mg/L (ppm)
Executive summary:

The ECOSAR QSAR model predicts that the 96hr LC50 for freshwater fish would to be 943.623 mg/l therefore that the substance is effectively not toxic to fishes.

Endpoint:
short-term toxicity to fish
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.
Qualifier:
according to guideline
Guideline:
other: QSAR
Principles of method if other than guideline:
EPI Suite v 4.1/ECOSARv1.00
GLP compliance:
no
Remarks:
not applicable to QSAR models
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
other: Fishes species
Details on test organisms:
no further information
Test type:
other: QSAR model
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
not applicable. QSAR model
Test temperature:
not applicable. QSAR model
pH:
not applicable. QSAR model
Dissolved oxygen:
not applicable. QSAR model
Salinity:
not applicable. QSAR model
Details on test conditions:
100% Sodium ethyl xanthate (CAS No. 140-90-9)
SMILES : S=C([S-])OCC.[Na+]
CHEM : Carbonodithioic acid, o-ethyl ester, sodium salt
MOL FOR: C3 H5 O1 S2 Na1
MOL WT : 144.18
Log Kow: 0.87 (used)
Wat Sol: 1.79E+004 mg/L (WskowWin estimate)





Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1 416.171 mg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
not specified
Details on results:
Using the EPIWIN ECOSAR QSAR model, a 96hr LC50 for marine water fish of 1416.171mg/l was established.
Fish, 96-hr LC50 =1416.171 mg/L (ppm)
Validity criteria fulfilled:
yes
Remarks:
US EPA accepted QSAR method for chemicals properties assessment.
Conclusions:
Using the EPIWIN ECOSAR QSAR model, a 96hr LC50 for marine water fish of 1416.171 mg/l was established.
Fish, 96-hr LC50 =934.623 mg/L (ppm)
Fish, 96-hr (SW) LC50 =1416.171 mg/L (ppm)
Fish 14-day LC50 = 927.914 mg /L (ppm)
Fish 30-day ChV = 90.256 mg/L (ppm)
Fish (SW) 30-day ChV = 70.993 mg/L (ppm)
Executive summary:

The ECOSAR QSAR model predicts that the 96hr LC50 for marine water fish would to be 1416.171 mg/l therefore that the substance is effectively not toxic to fishes.

Description of key information

Under the conditions of the study of Fuerstenau,M.C., B.M. Wakawa, R.K. Price, and R.D. Wellik,1974, Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 2.6 mg/l (NOEC). One of the 96h-LC50 was between 13 and 15mg/l .

The ECOSAR QSAR model predicts that the 96hr LC50 for marine water fish (SW) would to be 1416.171 mg/l therefore that the substance is effectively not toxic to fishes.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
13 mg/L

Marine water fish

Marine water fish
Effect concentration:
1 416.171 mg/L

Additional information

Under the conditions of the study of Fuerstenau,M.C., B.M. Wakawa, R.K. Price, and R.D. Wellik,1974, Sodium ethyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 2.6 mg/l (NOEC). One of the 96h-LC50 was between 13 and 15mg/l .

The ECOSAR QSAR model predicts that the 96hr LC50 for marine water fish (SW) would to be 1416.171 mg/l therefore that the substance is effectively not toxic to fishes.