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EC number: 223-564-1 | CAS number: 3962-66-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 Dec 2019 - 29 Feb 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Qualifier:
- according to guideline
- Guideline:
- other: Chemicals Fish Acute Toxicity Test (GB/T 27861-2011)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidelines for the Testing of Chemicals (HJ/T 153-2004)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- The study references to Procedure 203 of the Guidelines for the Testing of Chemicals of the OECD: Fish, Acute Toxicity Test (2019).
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- According to GLP standards of China.
- Analytical monitoring:
- yes
- Details on sampling:
- During the actual test, HPLC was used to analyse the test substance concentration in water samples at 0, 24, 48, 72, and 96 hours.
- Concentrations: 19.8%, 29.7%, 44.5%, 66.7%, and 100% saturation concentrations
- Sampling method: 10.0 mL test solution was taken from the blank, 19.8%, 29.7%, 44.5%, and 66.7% saturated solutions, passed through 0.22 μm aqueous membrane, the first 1 mL filtrate was discarded, then 1.00 mL filtrate was collected, and used in HPLC analysis. 1.00 mL of the 100% saturated solution was taken, diluted with acetonitrile to 10.0 mL (10 times dilution), passed through 0.22 μm organic membrane, the first 1 mL filtrate was discarded, then 1.00 mL filtrate was collected, and used in HPLC analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A saturated solution dilution method was used to prepare the test substance solutions. For the pre-test, 1.2236 g was weighed and test water was used to dilute to 12 L; for the actual test, 3.0582 g was weighed, and test water used to dilute to 30 L. It was then stirred in an airtight agitator, continuously for 24 hours, at 500 rpm, at 30 °C, with the height of the vortex generated while stirring being approximately one-tenth the height of the solution surface in a static state. Then it was set aside at room temperature for one hour and, after the solution was filtered through a 0.45-μm aqueous membrane (the initial 100 mL was discarded), it served as the test substance 100% saturated solution.
- Controls:
Blank control test: Under the same test conditions as the test substance treatment groups, a test water blank control test was carried out simultaneously.
Reference compound test: Under identical test conditions, potassium dichromate was used as the reference toxin in a reference compound test.
- Test concentration separation factor: 1.36 - 1.74 (based on measured concentrations)
- Evidence of undissolved material (e.g. precipitate, surface film, etc.):The saturated solution was colourless, and no undissolved materials were observed under microscopy. - Test organisms (species):
- other: Gobiocypris rarus
- Details on test organisms:
- TEST ORGANISM
- Common name: Chinese rare minnow
- Source: The Gobiocypris rarus used in the test were produced and raised within the testing laboratory.
- Length at study initiation (length definition, mean, range and SD): 2.984 cm (relative standard deviation 4.89 %)
- Weight at study initiation (mean and range, SD): 0.2285 g (relative standard deviation 5.65 %)
ACCLIMATION
- Acclimation period: Prior to use in the test, the fish were acclimated in the test environment for seven days.
- Acclimation conditions (same as test or not): not the same:
Light: 12 hours light each day, intensity 877-926 lux;
Water quality: same water quality as the test water;
Temperature: 23.0-23.3 °C;
Dissolved oxygen: 103.8-107.0% of the air saturation value;
- Type and amount of food during acclimation as well as feeding frequency: They were fed newly hatched Artemia salina twice daily, terminating 24 hours prior to the start of the test.
- Health during acclimation (any mortality observed): The fish mortality rate in the seven-day acclimation period was 0.
FEEDING DURING TEST (as applicable)
No feeding. - Test type:
- static
- Water media type:
- freshwater
- Remarks:
- The water used in the test was tap water aerated and treated for 24 hours or longer.
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 149 mg CaCO3/L (measured in blank control at the start of the test)
- Test temperature:
- 22.7 - 24.0 °C
- pH:
- 7.03 - 7.36
- Dissolved oxygen:
- 73.7 - 107.2 %
- Nominal and measured concentrations:
- The results indicated that, in water samples from saturated solution concentrations at 19.8%, 29.7%, 44.5%, 66.7%, and 100% preparation concentrations, the concentrations of test substance measured in water samples were 2.38, 4.15, 5.66, 8.71, and 13.0 mg/L, respectively.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 15 L fish tanks (Haimen Haoqi Laboratory Instruments Factory)
- Material, size, fill volume: glass, 10 L
- Aeration: the water used in the test was aerated for 24 h or longer
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: Fish loading per unit of water was approximately 0.2285 g (wet weight)/L.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Particulate matter: < 4.00 mg/L
- Metals: Cadmium: < 0.5 µg/L; Lead: <2.5 µg/L; Cobalt: < 0.02 mg/L; Nickel: < 0.05 mg/L, Copper: 5.2 µg/L; Chromium6+: 0.004 mg/L; Iron: <0.01 mg/L; Zinc: <0.09 mg/L; Silver: <0.03 mg/L
OTHER TEST CONDITIONS
- Photoperiod: 12 hours daily
- Light intensity: 876 - 926 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 24 h-LC50 (reference substance), 96h-LC0, 96h-LC100, 96h-LC50 (test substance)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.36 - 1.74 (based on measured concentrations)
- Range finding study: Yes
- Test concentrations: 19.8%, 29.7%, 44.5%, 66.7%, and 100% saturated solution (corresponding to measured concentrations of 2.38, 4.15, 5.66, 8.71, and 13.0 mg/L, respectively)
- Results used to determine the conditions for the definitive study (determined in a pre-test): 10% and 100% saturated solution - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (purity: 99.9 %)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 6.6 mg/L
- 95% CI:
- >= 5.44 - <= 8
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 2.38 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other abnormalities: see 'Any other information on results incl. tables'
- Mortality of control: none
- Other adverse effects control: none
- Effect concentrations exceeding solubility of substance in test medium: none - Results with reference substance (positive control):
- - LC50 after 24 h: 254 mg/L (CI 237 - 271 mg/L)
- Sublethal observations / clinical signs:
Test Fish Fatalities (Actual Test)
Prepared concentration (mg/L) Measured concentration (mg/L) Number of fish (n) Number of dead fish (n) 3 h 6 h 24 h 48 h 72 h 96 h 0 (blank control) ND1) 10 0 0 0 0 0 0 19.8 % saturated solution 2.38 10 0 0 0 0 0 0 29.7% saturated solution 4.15 10 0 0 1 1 1 1 44.5% saturated solution 5.66 10 0 2 3 4 5 5 66.7% saturated solution 8.71 10 3 6 6 6 6 6 100% saturated solution 13.0 10 10 10 10 10 10 10 LC50 (mg/L) 7.10 6.85 6.60 6.60 95% confidence interval (mg/L) 5.89 - 8.56 5.65 - 8.29 5.44 - 8.00 5.44 - 8.00 ND: lower than the limit of quantitation (LOQ = 0.0300 mg/L).
Observation Results from Actual Test
Prepared concentration (mg/L) Measured concentration (mg/L) Observation results 3 h 6 h 24 h 48 h 72 h 96 h 0 (blank control) ND1) 101) NB1) 10 NB 10 NB 10 NB 10 NB 10 NB 19.8 % saturated solution 2.38 10 NB 10 NB 10 NB 10 NB 10 NB 10 NB 29.7% saturated solution 4.15 10 NB 9 NB & 1SR 9 NB & 1 death 9 NB & 1 death 9 NB & 1 death 9 NB & 1 death 44.5% saturated solution 5.66 10 NB 7 NB & 1 SR
& 2 deaths5 NB & 2 SR 5 NB & 1 SR
& 4 deaths5 NB & 5 deaths 5 NB & 5 deaths 66.7% saturated solution 8.71 5 NB & 2 SR & 3 deaths 4 NB & 6 deaths 4 NB & 6 deaths 4 NB & 6 deaths 4 NB & 6 deaths 4 NB & 6 deaths 100% saturated solution 13.0 10 deaths 10 deaths 10 deaths 10 deaths 10 deaths 10 deaths NB1) – normal activity; SR2) – fish lay facing up or sideways.
- Validity criteria fulfilled:
- yes
- Remarks:
- Validity was stated according to Chinese guidelines
- Conclusions:
- LC50 (96 h) = 6.60 mg/L (95% confidence interval was 5.44 - 8.00 mg/L (based on mean of actual measured concentrations))
LC0 (96 h) = 2.38 mg/L (based on mean measured concentration)
LC100 (96 h) = 13.0 mg/L (based on mean actual measured concentration) - Executive summary:
In accordance with Guidelines for the Testing of Chemicals (HJ/T 153-2004), Chemicals – Fish Acute Toxicity Test (GB/T 27861-2011), and Chemical Test Methods – Ecological System Effects Volume (second edition, Beijing: China Environment Press, 2013: 30-36), and with reference to Procedure 203 of the Guidelines for the Testing of Chemicals of the OECD: Fish, Acute Toxicity Test (2019), this project used the static method to measure the acute toxicity of the test substance in fish (Gobiocypris rarus). In the blank control group, the activity of the test fish was normal, and the 24 h-LC50 of the reference compound potassium dichromate in Gobiocypris rarus was 254 mg/L. In the 29.7% saturated solution concentration group (corresponding to 4.15 mg/L measured test substance) and higher concentration treatment groups, after the test fish came into contact with the test substance, they lay facing up or sideways. At the end of 96 hours of exposure, the test fish in the 100% saturated solution group (corresponding to 13 mg/L measured test substance) were all dead.
Following LC-values were obtained after 96h:
LC50 = 6.60 mg/L (95% confidence interval was 5.44 - 8.00 mg/L (based on mean of actual measured concentrations))
LC0 = 2.38 mg/L (based on mean measured concentration)
LC100 = 13.0 mg/L (based on mean actual measured concentration)
Reference
Description of key information
In accordance with Guidelines for the Testing of Chemicals (HJ/T 153-2004), Chemicals – Fish Acute Toxicity Test (GB/T 27861-2011), and Chemical Test Methods – Ecological System Effects Volume (second edition, Beijing: China Environment Press, 2013: 30-36), and with reference to Procedure 203 of the Guidelines for the Testing of Chemicals of the OECD: Fish, Acute Toxicity Test (2019), this project used the static method to measure the acute toxicity of the test substance in fish (Gobiocypris rarus). In the blank control group, the activity of the test fish was normal, and the 24 h-LC50 of the reference compound potassium dichromate in Gobiocypris rarus was 254 mg/L. In the 29.7% saturated solution concentration group (corresponding to 4.15 mg/L measured test substance) and higher concentration treatment groups, after the test fish came into contact with the test substance, they lay facing up or sideways. At the end of 96 hours of exposure, the test fish in the 100% saturated solution group (corresponding to 13 mg/L measured test substance) were all dead.
Following LC-values were obtained after 96h:
LC50 = 6.60 mg/L (95% confidence interval was 5.44 - 8.00 mg/L (based on mean of actual measured concentrations))
LC0 = 2.38 mg/L (based on mean measured concentration)
LC100 = 13.0 mg/L (based on mean measured concentration)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 6.6 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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