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EC number: 601-147-9 | CAS number: 111988-49-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 - 05 Jul 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
waste treatment plant Odenthal, Germany
- Method of cultivation: not reported
- Preparation of inoculum for exposure:
- Pretreatment: none
- Initial biomass concentration: 800 mg/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- 20 ± 2 °C
- pH:
- 8.0
- Nominal and measured concentrations:
- Nominal concentrations: 1000, 1800, 3200, 5600 and 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: not reported
- Material, size, headspace, fill volume: not reported
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- No. of vessels per abiotic control (replicates): 1 (10000 mg/L)
- Sludge concentration: 800 mg/L
- Nutrients provided for bacteria: not reported
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED: respiratory rate after 3 hours - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 6 330 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The abiotic O2-consumption was determined at a concentration of the test substance of 10000 mg/L. As no abiotic O2-consumption was determined at this concentration, also lower concentrations of the test substance do not cause O2-consumption.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes (EC50 (30 min) of 3,5-DCP should be in the range 2 – 25 mg/L
- Relevant effect levels: EC50 (30 min) = 14 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (30 min) of the test item towards activated sludge was 6330 mg/L (nominal) based on total respiration inhibtion.
Reference
Table 1: Results on respiratory rate in the test after 3 hours
Nominal Concentration [mg/L] |
Respiratory rate [mg/L*h] |
Abiotic O2consumption [mg/L*h] |
Respiratory abiotic O2consumption [mg/L*h] |
Inhibition [%] |
1000 |
50.0 |
0.0* |
50.0 |
16.7 |
1800 |
52.0 |
0.0* |
52.0 |
13.3 |
3200 |
50.0 |
0.0* |
50.0 |
16.7 |
5600 |
46.0 |
0.0* |
46.0 |
23.3 |
10000 |
7.3 |
0.0 |
7.3 |
87.8 |
Control |
60.0 |
|
* No abiotic O2consumption was determined at the highest test concentration. Lower concentrations of the test substance do not cause O2consumption either (postulated values).
Table 2: Validity criteria for OECD 209
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
75 mg O2/g |
yes |
The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. |
0% |
yes |
Description of key information
EC50 (30 min) = 6330 mg/L (nominal, activated sludge, ISO 8192)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 6 330 mg/L
Additional information
One study is available assessing the effects of the test item on microorganisms according to ISO 8192. In this key study, 800 mg/L of activated sludge (mixed population of different microorganisms) was treated with synthetic medium for 30 minutes and the respiratory rate was measured. This respiratory rate was compared with those measured in test batches containing different concentrations of the test substance (1000 - 10000 mg/L). The sensitivity of the activated sludge being used was checked with 3,5-dichlorphenol as reference substance. The EC50 (30 minutes) was determined to be 6330 mg/L (nominal). For the reference substance 3,5-dichlorphenol an EC50 (30 min) value of 14 mg/L (nominal) was determined.
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