Registration Dossier
Registration Dossier
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EC number: - | CAS number: -
Water solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- GLP compliance:
- no
- Type of method:
- flask method
- Specific details on test material used for the study:
- Lot PF 897
- Key result
- Water solubility:
- > 538 g/L
- Conc. based on:
- test mat.
- Temp.:
- 30 °C
- pH:
- ca. 7
- Remarks on result:
- other: The pH was not reported and was assumed to be 7
- Details on results:
- The highest concentration of ω-Transaminase in water is achieved at 538 mg/mL. At tis concentration, the solution is in a gel-like state. Adding additional lyophilized powder into this solution will make mixing impossible.
- Conclusions:
- ω-Transaminase is extremely soluble in water. It was not possible to measure the full extent of ω-Transaminase solubility as the solution becomes too viscous above 538 mg/mL and it cannot be mixed sufficiently to confirm all of the lyophilized powder was dissolved. Therefore, the water solubility of ω-Transaminase is reported as > 538 g/L.
Reference
The final concentration of ω-Transaminase is calculated based on the amount of lyophilized powder added and the final volume of the solution (Table 1). The UV280nm absorbance of each solution is measured to confirm that the OD is within linear range.
Table 1. Final volume and concentration of ω-Transaminase in water
Weight of ω-Transaminase (mg) | Water (mL) | Total Volume (mL) |
63.1 | 5.00 | 5.10 |
108.2 | 5.00 | 5.24 |
201.5 | 5.00 | 5.34 |
411.4 | 5.00 | 5.46 |
614.8 | 5.00 | 5.77 |
807.6 | 5.00 | 5.90 |
1508.8 | 5.00 | 6.28 |
2415.2 | 5.00 | 6.30 |
3522.0 | 5.00 | 6.54 |
4541.9 | 5.00 | 6.62 |
Description of key information
ω-Transaminase is extremely soluble in water. It was not possible to measure the full extent of ω-Transaminase as the solution becomes too viscous above 538 mg/mL and it cannot be mixed sufficiently to confirm all of the lyophilized powder was dissolved. Therefore, the water solubility of ω-Transaminase is reported as > 538 g/L.
Key value for chemical safety assessment
- Water solubility:
- 538 g/L
- at the temperature of:
- 30 °C
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
