Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-118-9 | CAS number: 676532-44-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 08 July 2016 to 21 September 2016
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: Ministry of environmental protection of the people's Republic of China, The Guidelines for the Testing of Chemicals - Effects on Biotic Systems, 2013, 207 Earthworm, Acute Toxicity Test
- Version / remarks:
- China Guideline
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Solubility in water: 0.014 g/L at 20°C
- Vapour pressure: 0.459 Pa at 20°C
- log Kow: 4.4
- Conditions of storage: Keep in dark place at room temperature (T: 10-30°C, H: <45%)
- Stability at conditions of storage: Dry, well ventilled, preferably full, hermetically sealed.
Batch No. : SC00017257
Purity : 97.6%
Date of Expiry : 15 April, 2018 - Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- The test substance may be volatile and was added into soil directly. An amount of the test substance was separately added to an appropriate amount of the silica sand, and then incorporation into formulated artificial soil with deionised reverse osmosis water. The treatment groups were prepared by the following test matrix component: See table 6.3.1/1 in "Any other information on materials and methods incl. tables". These were mixed by a mixer respectively with a nominal moisture of 40-60% by mass of the total water-holding capacity with the deionized water. The test matrix components were mixed evenly and distributed to 4 containers.
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- The earthworms were purchased from Jiang Su province Jurong Stock Farm. All newly acquired earthworms were quarantined and observed for 14 days prior to use in the test. Earthworms are bred on a substrate consisting of 50% by volume peat and 50% by volume cow manure and kept under 20 +/- 2°C for 7 days prior to testing. The test organisms were with clitellum and were transferred into artificial soil (without test substance) approximately 24 hours prior to the start of the test. At the start of the test, the adult earthworms used originated from the same population, were clitellum and wet weight of 300 to 600 mg.
The earthworms received no food during exposure. - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- The measured temperature of the climate chamber remained 20.7 to 21.0°C throughout the study, which was in the range of 18-22°C.
- pH:
- pH values were 5.98 to 6.40 at initiation and 6.01 to 6.38 at termination.
- Moisture:
- Soil moisture content were 26.04% to 29.42% at initiation and 26.38% to 29.35% at termination.
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1 litre closed glass vessels
- Amount of soil or substrate: Each container was loaded with approximately 750 g wet weight of the substrate.
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE :
A defined artificial soil was used as a basic test substrate with the following composition: Sphagnum peat 10% w/w; Kaolinite clay (Sigma-Aldrich) 20% w/w; Silica sand (screened with 0.2 mm sieve) 70% w/w.
The pH of the artifical soil was adjusted to 6.0 +/- 0.5. Before the start of the test, measured maximum water holding capacity (WHC) of artificial soil was 52%. Moisture content of the test substrate was adjusted to 40-60% by mass of the total WHC with the deionized water. Appropriate moisture content is 21-31%. The water content does not vary with more than 10% from that at the start of the test (OECD 222, 2004).
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity: 610-630 lux
EFFECT PARAMETERS MEASURED :
The total gross weights of the earthworms added to each vessel in the controls and the treatment groups were recorded on Day 0 before addition. The surviving earthworms in each vessel were weighted on Day 14.
Mortalities were recorded 7 and 14 days after the start of treatment. Earthworm and soil were poured out from glass containers to the stainless steel basin on 7 days, counting number, observation, and then after record the surviving earthworms and soil were put back into the corresponding container.
RANGE-FINDING TEST
In the range-finding test, the earthworms were exposed to a series of nominal concentrations of 10, 100 and 1000 mg/kg and blank control, in single replicate. The results showed no mortality occurred in the blank control group and in the 10 and 100 mg/kg treatment groups on the 7th day and 14th day, but in 1000 mg/kg on 7th day, the mortality was 80%, and on 14th day, the mortality was 100%. - Nominal and measured concentrations:
- The nominal concentrations of the treatment groups were 95, 153, 244, 391, 625 and 1000 mg test substance/kg dry artificial soil weight.
- Reference substance (positive control):
- yes
- Remarks:
- Chloroacetamide
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- 361 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI: 327 - 400 mg/kg
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 291 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI: 269 - 315 mg/kg
- Details on results:
- - Mortalities: See table 6.3.1/2 in "Any other information on results incl. tables". Mortalities in control, 95 and 153 mg/kg group on 7th and 14th day both were 0%. Mortalities in 224 mg/kg group on the 7th day were 17.5% and 14th day were 22.5%. Mortalities in 391 mg/kg group on 7th were 60% and 14th day were 90%. Mortalities in 625 mg/kg group on 7th were 90% and 14th day were 100%. Mortalities in 1000 mg/kg group on 7th and 14th day were 100%.
- Behavioural or pathological symptoms: None
- Body weight: See Table 6.3.1/3 in "Any other information on results incl. tables". - Results with reference substance (positive control):
- The validity of the test system and Eisenia fetida is regularly confirmed by determining the LC50 against a reference substance. For this purpose, chloroacetamide was employed. The most recent test was performed from 19th Jan 2016 to 2th Feb 2016 (14 days test). In this study, the reference substance was tested at 5 concentrations of 3.75, 7.50, 15, 30 and 60 mg/kg dry artificial soil weight.
Mortalities were shown to be between 22.5 to 95%. The 14d-LC50 for chloroacetamide was 25.7 mg/kg dry soil weight.
- Results with reference substance valid? yes - Validity criteria fulfilled:
- yes
- Remarks:
- The mortality of the earthworm in the blank control group was 0%, which was not more than 10%, so the test was valid.
- Conclusions:
- Under the conditions of the test, the 7d-LC50 and 14d-LC50 values, for the test substance to earthworm (Eisenia fetida) based on the nominal test concentrations, were 361 mg/kg (95% CI: 327 - 400 mg/kg) and 291 mg/kg (95% CI: 269 - 315 mg/kg) dry soil weight, respectively.
- Executive summary:
The acute toxicity of the test substance to Eisenia fetida was determined in a 14 days artificial soil test, according to "Ministry of environmental protection of the people's Republic of China, The Guidelines for the Testing of Chemicals - Effects on Biotic Systems, 2013, 207 Earthworm, Acute Toxicity Test" and OECD Guideline 207 (1984) with GLP compliance.
Based on the results of range-finding test, 6 treatment groups and one blank control group, which had four replicates of 10 worms per concentration were included in definitive test. The nominal concentrations of the treatment groups were 95, 153, 244, 391, 625 and 1000 mg/kg dry artificial soil weight. The number of mortalities were determined after 7 and 14 days of exposure.
The mortality of the earthworm in the blank control group was 0%, which was not more than 10%, so the test was valid.
Under the conditions of the test, the 7d-LC50 and 14d-LC50 values, for the test substance to earthworm (Eisenia fetida) based on the nominal test concentrations, were 361 mg/kg (95% CI: 327 - 400 mg/kg) and 291 mg/kg (95% CI: 269 - 315 mg/kg) dry soil weight, respectively.
Reference
Table 6.3.1/2: The mortality of Eisenia fetida exposed to the test substance and control at 7 and 14 days after application in definitive test
Parameter |
Blank control |
Treatments (mg/kg dry soil weight) |
||||||||||||
95 |
153 |
244 |
391 |
625 |
1000 |
|||||||||
Duration (d) |
7 |
14 |
7 |
14 |
7 |
14 |
7 |
14 |
7 |
14 |
7 |
14 |
7 |
14 |
Number of earthworm |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
Number of mortality (A replicate) |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
7 |
10 |
9 |
10 |
10 |
10 |
Number of mortality (B replicate) |
0 |
0 |
0 |
0 |
0 |
0 |
4 |
5 |
8 |
10 |
8 |
10 |
10 |
10 |
Number of mortality (C replicate) |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
6 |
10 |
9 |
10 |
10 |
10 |
Number of mortality (D replicate) |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
3 |
3 |
6 |
10 |
10 |
10 |
10 |
Total |
0 |
0 |
0 |
0 |
0 |
0 |
7 |
9 |
24 |
36 |
36 |
40 |
40 |
40 |
Mortality (%) |
0 |
0 |
0 |
0 |
0 |
0 |
17.5 |
22.5 |
60 |
90 |
90 |
100 |
100 |
100 |
Table 6.3.1/3: Number and average body weight of the surviving earthworms at the beginning and the end of the definitive test
Groups |
No. of worms Day 0 |
Mean weight Day 0 (mg) |
No. of worms Day 14 |
Mean weight Day 14 (mg) |
Weight reduction 14 days (%) +/- standard deviation |
||
Blank control |
A B C D |
10 10 10 10 |
573 552 520 523 |
10 10 10 10 |
415 465 488 438 |
16.43 +/- 8.76 % |
|
Treatment groups (mg/kg dry soil weight) |
95 |
A B C D |
10 10 10 10 |
538 521 528 543 |
10 10 10 10 |
489 416 465 470 |
13.66 +/- 4.69 % |
153 |
A B C D |
10 10 10 10 |
526 530 552 565 |
10 10 10 10 |
490 428 437 421 |
18.1 +/- 7.96 % |
|
244 |
A B C D |
10 10 10 10 |
532 536 544 526 |
9 5 10 7 |
434 416 472 366 |
21.11 +/- 7.28 % |
|
391 |
A B C D |
10 10 10 10 |
534 538 570 541 |
0 0 0 4 |
- - - - |
- |
|
625 |
A B C D |
10 10 10 10 |
566 521 563 534 |
0 0 0 0 |
- - - - |
- |
|
1000 |
A B C D |
10 10 10 10 |
535 526 561 557 |
0 0 0 0 |
- - - - |
- |
Description of key information
OECD Guideline 207, GLP, key study, validity 2:
14d-LC50 (Eisenia fetida) = 291 mg/kg dw of soil (95% CL: 269 - 315 mg/kg dw of soil).
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 291 mg/kg soil dw
Additional information
To assess the toxicity of the registered substance to soil macroorganisms, one experimental study is available.
This study was performed according to OECD Guideline No. 207 with GLP compliance to determine the acute toxicity of the registered substance to adult earthworms Eisenia fetida in an artificial soil. Following a preliminary range-finding test, 40 earthworms (4 replicates of 10 worms) were exposed to nominal test concentrations of 95, 153, 244, 391, 625 and 1000 mg/kg dw of soil for a period of 14 days. The number of mortalities were determined after 7 and 14 days exposure. The 14-d LC50 for the test substance to earthworms based on nominal test concentrations was 291 mg/kg dw of soil (95% CL: 269 - 315 mg/kg dw of soil).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.