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EC number: 221-391-6 | CAS number: 3084-40-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- For the test group (100 mg/L), six Daphnia were added to one replicate vessel instead of 5. The addition of an extra daphnid had no impact on either the validity or integrity of the study.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- yes
- Remarks:
- see above
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The test concentration of E06-16 was measured using liquid chromatography with Mass Spectrometric detection (LC-MS). At the start of the definitive test, four samples (10 mL) were taken from the freshly-prepared control and test media. After 48 ours, the contents of the test vessels from the control and test group were pooled and further samples were taken for analysis. On each occasion, two of the samples was analysed and the other was stored in a freezer in case further analysis was required.
- Vehicle:
- no
- Details on test solutions:
- The method of preparation used during the definitive test was based on the results of a formulation trial and a range finding test.
The test substance (100 mg) was added to dilution medium (ca. 700 mL) in a volumetric flask (1 L), made up to volume and vigorously mixed to provide test medium with a nominal concentration of 100 mg/L as E06-16. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna (Straus) were cultured in-house and were obtained from a strain originating from the National Institute for Applied Chemical Research (IRCHA), France. Cultures were fed daily with a suspension of the unicellular green algae, Pseudokirchneriella subcapitata, to provide nominally 0.1 to 0.2 mg carbon per daphnid, per day, except during the initial two days when a slightly lower ration was given. Culture conditions ensure that the stock animals reproduce by parthenogenesis.
The day before the start of the study, all juvenile Daphnia were removed from the laboratory cultures. The following morning, juveniles produced by the gravid (egg-bearing) adult Daphnia were removed from the culture vessels and held in a separate holding vessel; these animals, which were less than 24 hours old, were used in the test. - Test type:
- static
- Water media type:
- other: Elendt M4 medium
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- The range finding test employed nominal test concentrations of 0.1, 1, 10 and 100 mg/L as E06-16. After 48 hours, there was no observed immobilisation in any of the test groups. Based on this result, the definitive test employed a single nominal concentration (limit test) of
100 mg/L as E06-16. - Hardness:
- The total hardness and alkalinity of the batch of Elendt M4 medium used were 253 and 48 mg/L as CaCO3,
respectively - Test temperature:
- Control: 21.0 - 22.0 °C
Treatment: 21.1 - 22.2 °C - pH:
- Control: 8.01 - 8.18
Treatment: 8.22 - 8.23 - Dissolved oxygen:
- around 9 mg/L
- Nominal and measured concentrations:
- 100 and 112 mg/L respectively
- Details on test conditions:
- Twenty Daphnia, four replicates of five animals per vessel, were exposed in each control and test group. Daphnia were exposed to the test or control conditions for a period of 48 hours without renewal of test medium. During the definitive test, continuous monitoring of the medium in an additional vessel indicated that temperatures ranged between 21.3 to 22.4°C. A photoperiod of 16 hours light : 8 hours dark was maintained. No supplementary aeration was employed and no feed was given during the exposure period.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- other: EC50 and NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- other: EC50 and NOEC
- Effect conc.:
- > 112 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The intended exposure concentration of E06-16 was adequately achieved (with replicate samples ranging between 106% and 116% of its nominal value) and maintained during the test (108% of its starting value). The overall mean measured concentration of E06-16 was 112 mg/L and this value has been used in the determination of study end-points. There was no immobilisation observed during the test, giving a 48 hour EC50 of greater than 112 mg/L, and a NOEC (no observed effect concentration) of 112 mg/L (measured).
- Results with reference substance (positive control):
- n/a
- Reported statistics and error estimates:
- The intended exposure concentration of E06-16 was adequately achieved (with replicate samples ranging between 106% and 116% of its nominal value) and maintained during the test (108% of its starting value). The overall mean measured concentration of E06-16 was 112 mg/L and this value has been used in the determination of study end-points.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the acute toxicity of E06-16 to Daphnia magna the test substance was found to be not acutely toxic at a mean measured concentration of 112 mg/L.
- Executive summary:
The acute toxicity of E06-16 to Daphnia magna was assessed under static exposure conditions over a period of 48 hours. E06-16 was not found to be acutely toxic to Daphnia magna at a mean measured concentration of 112 mg/L.
Based on these findings, the 48-hour EC50 for immobilisation could not be calculated but must be greater than 112 mg/L (measured) and the no-observed-effect concentration (NOEC) was 112 mg/L (measured).
Reference
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 112 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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