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EC number: 244-320-0 | CAS number: 21295-57-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2020-06 till 2020-11-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling schedule:
Control : on day 0, 7 and 17 in the freshly prepared media;
on day 3, 10 and 19 in the old media
Test concentrations : all test concentrations on day 0, 7 and 17 in the freshly prepared
media; on day 3, 10 and 19 in the old media - Vehicle:
- no
- Details on test solutions:
- Calibration solutions
For the calibration, 21.1 mg of the test item was dissolved in acetonitrile/tetrahydrofuran 8/2 and then made up with the same solvent to the mark in a 100 mL volumetric flask (211.0 mg/L). This stock solution was diluted further with acetonitrile to prepare an intermediate solution (1055.0 µg/L). This intermediate solution was diluted further to prepare calibration solutions between 0.0528 µg/L and 52.75 µg/L test item. The calibration solutions were analysed.
Standard Solutions used for Verification of the Calibration and Blank Test
For the verification during the study, 22.43 mg of the test item was dissolved in acetonitrile/tetrahydrofuran 8/2 and then made up with the same solvent to the mark in a 100 mL volumetric flask (224.3 mg/L). This stock solution was diluted further with acetonitrile to prepare one verification solution (4.206 µg/L). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Young female Daphnia, aged less than 24 hours at the start of the test
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 14.2 °dH to 15.6 °dH
(= 253 - 278 mg/L CaCO3) - Test temperature:
- 18 - 22 °C
- pH:
- The pH was within the range 6 - 9.
- Nominal and measured concentrations:
- The following nominal concentrations of the test item were tested in the acute test: 1, 10 and 100 mg/L (preciding range finder for the reproduction test). In the reproduction test the following nominal concetrations of the test item were tested: 0.0063, 0.013, 0.025, 0.050 and 0.1 mg/L.
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.005 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- Nominal concentration Analytical values
(time-weighted
mean)
[mg/L] [mg/L]
21d-NOEC (reproduction) (tα 0.05) : 0.025 0.0050
21d-LOEC (reproduction) (tα 0.05) : 0.050 0.0086
21d-NOEC (mortality) (tα 0.05) : ≥ 0.100 ≥ 0.019
21d-LOEC (mortality) (tα 0.05) : > 0.100 > 0.019 - Validity criteria fulfilled:
- yes
- Conclusions:
- After A 21 day period, a NOEC of 0.005 mg/l (measured concentration) was obtained on the basis of reproduction.
- Executive summary:
After a 21d-exposure period, the total number of living offspring produced per parent animal which did not die accidentally or inadvertently during the test was assessed. In addition, the reproduction was calculated based on the production of living offspring by the surviving parent organism. The reproductive output of the Daphnia exposed to the test item was compared to that of the control in order to determine the Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) according to Williams Multiple Sequential t-Test procedure. Additionally, the survival of the parent Daphnia and the time to production of first brood was determined.
Results [mg/L]
Nominal concentration
[mg/L]
Analytical values
(time-weighted mean)
[mg/L]21d-NOEC (reproduction) (tα 0.05) 0.025 0.0050 21d-LOEC (reproduction) (tα 0.05) 0.050 0.0086 21d-NOEC (mortality) (tα 0.05) ≥ 0.100 ≥ 0.019 21d-LOEC (mortality) (tα 0.05) > 0.100 > 0.019
Reference
Description of key information
After A 21 day period, a NOEC of 0.005 mg/l (measured concentration) was obtained on the basis of reproduction.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- 0.005 mg/L
Additional information
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