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EC number: 211-806-9 | CAS number: 697-82-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Long term toxicity to Chlorella autofrophica study was carried out for 3-7 days.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 2,3,5Trimethylphenol
- Molecular formula (if other than submission substance): C9H12O
- Molecular weight (if other than submission substance): 136.193 g/mol
- Smiles notation (if other than submission substance): c1(c(cc(C)cc1O)C)C
- InChl (if other than submission substance): 1S/C9H12O/c1647(
2)8(3)9(10)56/h45,10H,13H3
- Substance type: Organic
- Physical state: Solid
- Analytical purity: >95% - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: Test chemical conc. used for the study were 0.001, 0.01, 0.05, 0.1, 0.5, 1, 2 and 10 mg (1, 10, 50, 100, 500, 1000, 2000, 10,000 µg), respectively.
- Sampling method: Test solutions were prepared in absolute ethanol. - Vehicle:
- yes
- Remarks:
- absolute ethanol was used as a vehicle in the study.
- Test organisms (species):
- other: Chlorella autofrophica
- Details on test organisms:
- TEST ORGANISM
- Common name: Green Algae
- Strain: Strain 580
- Source (laboratory, culture collection): Test organism Chlorella autofrophica strain 580 was obtained from R. L. Guillard, Woods Hole.
- Method of cultivation: Chlorella autofrophica was grown on medium ASP-2 plus 8 µg/l. vitamin Blz and 1 mg/l. vitamin B1
- Other: The inoculum was preconditioned to the test conditions. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 7 d
- Remarks on exposure duration:
- Exposure duration is 3-7 days
- Test temperature:
- 28 - 30°C
- Nominal and measured concentrations:
- nominal concentration
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Petridish
- Initial cells density: 105 cells/ml
- Final cell conc: 5000-10000
cell/ml
GROWTH MEDIUM
- Standard medium used: No
- Detailed composition if non-standard medium was used: 1% Difco agar, 0140
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity and quality: continuous illumination from Sylvania F20T12-D Daylight fluorescent lamps
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The experimental endpoint was the zone size of growth inhibition around the pad, judged visually and microscopically.
TEST CONCENTRATIONS
- Test concentrations: Test chemical conc. used for the study were 0.001, 0.01, 0.05, 0.1, 0.5, 1, 2 and 10 mg (1, 10, 50, 100, 500, 1000, 2000, 10,000 µg), respectively. - Key result
- Duration:
- 7 d
- Dose descriptor:
- EC100
- Effect conc.:
- 10 other: mg
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Growth inhibition
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on growth inhibition of test organism, the EC100 value for chemical 2,3,5-Trimethylphenol was determined to be 10 mg.
- Executive summary:
Long term toxicity to Chlorella autofrophica study was carried out for 3-7 days.Chlorella autofrophica was used as a test organism. Test organismChlorella autofrophicastrain 580was obtained fromR. L. Guillard, Woods Hole.Chlorella autofrophicawas grown onmedium ASP-2 plus 8µg/l. vitamin Blz and 1 mg/l. vitamin B1. An algal lawn technique was used to test pure compounds. Exponentially growing cells (final concentration 5000-10000 cell/ml) were added to agarized medium (1% Difco agar, 0140) held at 42°C ;20 ml was then immediately distributed to plastic Petri dishes. The test materials, with absolute ethanol as solvent, were presented to the algal cells embedded in the agar by absorbing them on antibiotic sensitivity discs (12.7 mm) and placing the discs directly on the agar surface. The plates were then sealed with Scotch tape and incubated in the light for 3-7 days, 28-30°C. The experimental endpoint was the zone size of growth inhibition around the pad, judged visually and microscopically. No inhibition was observed with appropriate ethanol controls.Based on growth inhibition of test organism, the EC100 value for chemical 2,3,5-Trimethylphenol was determined to be 10 mg. Based on this value it can be concluded that the substance 2,3,5-trimethylphenol is considered to be toxic to aquatic environment. Since the test chemical is readily biodegradable in nature, chemical 2,3,5-trimethylphenol can be considered as non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
Reference
Table: Effect of test chemical on growth, as a lawn, of green alga,Chlorella autofrophicaStrain 580.
Amount on pad,µg |
Zone of inhibition (mm) |
1 |
0 |
10 |
0 |
50 |
0 |
100 |
0 |
500 |
2 (10) |
1000 |
20 (36) |
2000 |
36 |
10000 |
36 |
Note: Number indicates zone of inhibition of growth around pad in mm; 36 mm means no growth in Petri dish.Numbers in parentheses are zone sizes of reduced colony size indicative of some but not complete inhibition.
Description of key information
Long term toxicity to Chlorella autofrophica study was carried out for 3-7 days Kenneth Winters et. al; 1977). Chlorella autofrophicawas used as a test organism. Test organismChlorella autofrophicastrain 580was obtained fromR. L. Guillard, Woods Hole.Chlorella autofrophicawas grown onmedium ASP-2 plus 8µg/l. vitamin Blz and 1 mg/l. vitamin B1. An algal lawn technique was used to test pure compounds. Exponentially growing cells (final concentration 5000-10000 cell/ml) were added to agarized medium (1% Difco agar, 0140) held at 42°C ;20 ml was then immediately distributed to plastic Petri dishes. The test materials, with absolute ethanol as solvent, were presented to the algal cells embedded in the agar by absorbing them on antibiotic sensitivity discs (12.7 mm) and placing the discs directly on the agar surface. The plates were then sealed with Scotch tape and incubated in the light for 3-7 days, 28-30°C. The experimental endpoint was the zone size of growth inhibition around the pad, judged visually and microscopically. No inhibition was observed with appropriate ethanol controls.Based on growth inhibition of test organism, the EC100 value for chemical 2,3,5-Trimethylphenol was determined to be 10 mg. Based on this value it can be concluded that the substance 2,3,5-trimethylphenol is considered to be toxic to aquatic environment. Since the test chemical is readily biodegradable in nature, chemical 2,3,5-trimethylphenol can be considered as non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Additional information
Various experimental key study and predicted data for the target compound2,3,5-trimethylphenol(CAS no. 697-82-5) which is supported further by 1 study (from peer reviewed journal) for its closest read across substance with logKow as the primary descriptor were reviewed for the toxicity to aquatic algae and cyanobacteria end point which are summarized as below:
In an experimental key study for test chemical 2,3,5-trimethylphenol(CAS no. 697-82-5), long term toxicity to Chlorella autofrophica study was carried out for 3-7 days (Kenneth Winters et. al; 1977). Chlorella autofrophica was used as a test organism. Test organism Chlorella autofrophica strain 580 was obtained from R. L. Guillard, Woods Hole.Chlorella autofrophicawas grown onmedium ASP-2 plus 8µg/l. vitamin Blz and 1 mg/l. vitamin B1. An algal lawn technique was used to test pure compounds. Exponentially growing cells (final concentration 5000-10000 cell/ml) were added to agarized medium (1% Difco agar, 0140) held at 42°C ;20 ml was then immediately distributed to plastic Petri dishes. The test materials, with absolute ethanol as solvent, were presented to the algal cells embedded in the agar by absorbing them on antibiotic sensitivity discs (12.7 mm) and placing the discs directly on the agar surface. The plates were then sealed with Scotch tape and incubated in the light for 3-7 days, 28-30°C. The experimental endpoint was the zone size of growth inhibition around the pad, judged visually and microscopically. No inhibition was observed with appropriate ethanol controls.Based on growth inhibition of test organism, the EC100 value for chemical 2,3,5-Trimethylphenol was determined to be 10 mg. Based on this value it can be concluded that the substance 2,3,5-trimethylphenol is considered to be toxic to aquatic environment. Since the test chemical is readily biodegradable in nature, chemical 2,3,5-trimethylphenol can be considered as non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
Short term toxicity on aquatic algae and cyanobacteria of target chemical 2,3,5-trimethylphenol(CAS no. 697-82-5)is predicted using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances (2017). EC50 value was estimated to be 17.71 mg/l for Pseudokirchneriella subcapitata for 72 h duration. Based on this value it can be concluded that the substance 2,3,5-trimethylphenol is considered to be toxic to aquatic environment. Since the test chemical is readily biodegradable in nature, chemical 2,3,5-trimethylphenol can be considered as non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
Another short term toxicity study to Selenastrum capricornutum (algae) of the read across chemical 2, 3-dimethyl phenol (CAS no. 526-75-0) was carried out for 72 hrs (A. Kahru et. al; 2000). The initial algal culture was prepared from the immobilized algal beads and the deimmobilized cells were pregrown in the sterile growth medium (25 °C, 6000-8000 lux). Before the experiment the culture was diluted to OD670=0.001 corresponds to ~10⁴ cells/ml. and cultivated further for 4 days. The activated culture was used as the inoculum for the toxicity experiments. Algae were incubated with 2, 3-dimethyl phenol at 25°C for 72 h. The EC50 value of 2, 3-dimethyl phenol is considered to be 50mg/L in Selenastrum capricornutum. Based on this value it can be concluded that the substance 2,3 -dimethylphenol is considered to be toxic to aquatic environment. Since the test chemical is readily biodegradable in nature, chemical 2, 3-dimethyl phenol can be considered as non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.
Thus, based on the overall reported results for target chemical2,3,5-trimethylphenol(from peer reviewed journal and OECD QSAR toolbox version 3.3) and for its read across chemical (from peer reviewed journal) , it can be concluded that the test substance2,3,5-trimethylphenolcan be considered as non-toxic to aquatic environment and thus can be considered to be not classified as per the CLP classification criteria.
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