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EC number: 203-867-5 | CAS number: 111-41-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 Nov 2020 - 24 Dec 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
- Report date:
- 2021
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-(2-aminoethylamino)ethanol
- EC Number:
- 203-867-5
- EC Name:
- 2-(2-aminoethylamino)ethanol
- Cas Number:
- 111-41-1
- Molecular formula:
- C4H12N2O
- IUPAC Name:
- 2-[(2-aminoethyl)amino]ethan-1-ol
- Test material form:
- liquid: viscous
- Details on test material:
- Physical Description: Colourless viscous liquid
Purity/Composition correction factor: No correction factor require
Storage Conditions: At room temperature protected from light container flushed with nitrogen
Test item handling: Use amber glassware or wrap container in aluminum foil
Constituent 1
- Specific details on test material used for the study:
- The stability of the test substance was determined using Infra Red (CRL study no. 20230572). For the ready biodegradability study an infrared absorption spectrum was obtained at the end of the study. The infrared absorption spectrum was comparable to other spectra tested in this study. From this it could be concluded that the test item was stable during the ready biodegradability study.
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Storage conditions: The freshly obtained sludge was kept under continuous aeration until further treatment.
- Concentration of suspended solids: 3.07 g/L in the concentrated sludge 30.7 mg suspended solids/L in the test vessels.
- Preparation of inoculum for exposure: The sludge was coarsely sieved (1 mm). - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 79 mg/L
- Based on:
- ThOD
- Initial conc.:
- 29 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of the mineral medium: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon. Mineral medium, prepared according to OECD 301A
- Test temperature: 22°C
- pH at test start: 7.4-7.6
- pH at test end: 7.2 - 8.3
- pH adjusted: no
- Suspended solids concentration: the concentration of suspended solids (SS) was determined to be 3.07 g/L in the concentrated sludge as used for the test. The sludge was used as inoculum at a concentration of 10 mL per liter of mineral medium, leading to a final concentration SS of 30.7 mg/L. The SS was slightly above the 30 mg/L and the test item could not be classified as readily biodegradable in this best case situation, therefore there was no impact on the overall conclusion of the test item in the current study.
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 500 mL amber glass bottles. The end volume of medium was 244 mL, in order to obtain the required headspace-to-volume ratio.
- Number of culture flasks/concentration:
Test suspension: containing test item and inoculum (2 bottles).
PREPARATION OF THE TEST VESSELS
A stock solution of 1 g/L was prepared by adding a weight amount of 250 mg of test item in mineral medium. After stirring for 30 minutes, the final stock solution was clear and colourless, Aliquots of 7 ml of the stock solution were added to the test item bottles an the toxicity control.
At the start of the test (Day 0), test and/or procedural control item were added to the respective bottles. The volumes of suspensions were made up to 244 mL using inoculated mineral medium.
Rubber gaskets containing an appropriate amount of CO2 absorber were inserted into each bottle. Subsequently, each bottle was sealed by screwing an automated respirometer head on top.
- CO2 Absorber: Soda lime pellets (Merck, Darmstadt, Germany).
- Measuring equipment: Lovibond BD600-GLP manometric respirometry system, equipped with an inductive stirring system.
- Details of trap for CO2: The CO2 produced in each test bottle reacted with the CO2 absorbent in the rubber gaskets. As gaseous O2 was converted into gaseous CO2 that was absorbed, the gas pressure in the test system slowly decreased. This decrease in air pressure was measured by the respirometer heads and automatically converted into oxygen consumption (mg O2/L). Measurements were recorded automatically for 28 days, two times a day.
CONTROL AND BLANK SYSTEM
- Inoculum blank: containing only inoculum (2 bottles).
- Procedural control: containing procedural control item and inoculum (1 bottle).
- Toxicity control: containing inoculum, test item and procedural control item in amounts contributing equally to the total ThOD (1 bottle).
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 40
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 45
- Sampling time:
- 28 d
- Details on results:
- The relative biodegradation values calculated from the measurements performed during the test period revealed 45% and 40% biodegradation of the test item based on ThOD, for the duplicate bottles tested.
In the toxicity control, more then 25% biodegradation occurred within 14 days (52% based on ThOD). Therefore, the test item was considered not to inhibit microbial activity.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See overall remarks, attachments
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- 2-(2-aminoethylamino)ethanol was not readily biodegradable under the conditions of the Manometric Respirometry Test.
- Executive summary:
The test item 2-(2-aminoethylamino)ethanol was evaluated for its ready biodegradability in an aerobic aqueous medium with microbial activity introduced by inoculation with activated sludge., according to OECD guideline 301F and GLP principles. The test item was tested in duplicate at a target concentration of 29 mg/L, corresponding to a ThOD of 79 mgO2/L. Additionally two inoculum blanks, one procedural control and one toxicity control were tested. The relative biodegradation values calculated from the measurements performed during the test period revealed 40 % and 45 % biodegradation of the test item based on ThOD, for the duplicate bottles tested. In the toxicity control, 52% biodegradation occurred within 14 days. Therefore, the test item was considered not to inhibit microbial activity.
In conclusion, 2-(2-aminoethylamino)ethanol was not readily biodegradable under the conditions of the test presently performed. The study fulfilled all validity criteria of OECD 301F and is considered reliable without restrictions.
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