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EC number: 816-312-2 | CAS number: 92343-70-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jul 04 - Sep 17, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 22 Oct 2010
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 30 May 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Appropriate amounts of test item were directly weight into the test vessels and test water was added. The composition was stirred intensively
- Controls: pure water, synthetic sewage and inoculum, but without addition of test item - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Test System: Activated sludge
Origin: Municipal sewage treatment plant of Darmstadt, Germany
Reason for the selection: Non-adapted activated sludge from the sewage plant at Bensheim is well suited as it receives predominantly municipal sewage and hardly any industrial chemical waste.
Pretreatment: The activated sludge used for this study was used as collected, but coarse particles were removed by settling for a short period (15 minutes) and then the upper layer decanted.
During holding prior to use the sludge was fed with 50 mL synthetic sewage feed (see below) per litre and kept aerated at room temperature overnight. An aliquot of the final sludge suspension was weighed, dried
and the ratio of wet sludge to its dry weight determined.
Based on the sludge dry matter, calculated amounts of wet sludge were suspended in pure water to yield a concentration equivalent to 3 g/L on dry weight basis. This level gives a concentration of 1.5 g/L suspended solids in the
test medium. The pH of the activated sludge inoculum was 6.9 and therefore no adjustment necessary. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20.4°C
- pH:
- control: 7.3 (start) to 8.6 (end)
test item: 7.3 (start) to 8.6 (end) - Dissolved oxygen:
- Control samples : 6.9 mg O2/L
- Nominal and measured concentrations:
- test item: 100 mg/L (nominal)
positive control: 1, 4 and 16 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass flasks (approx. 1 litre) and Karlsruher flasks (250 mL)
- Type: open
- Aeration:compressed air (1.017 litre per minute)
- No. of vessels per test item concentration (replicates): 1
- No. of vessels per positive control concentration (replicates): 1
- No. of vessels of control (replicates): 2
- Sludge concentration (weight of dry solids per volume): 3.5 g/L
- Nutrients provided for bacteria: no
- Nitrification inhibitor used: N-allylthiourea
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Test item concentrations: 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- At a nominal test concentration of 100 mg test item/L less than 10% inhibition was observed after three hours of
incubation. The mean deviation in respiration rate was 4.2% at three replicates of 100 mg of the test item.
The respiration rate of the activated sludge to the test material at the rate of 100 mg/L was not statistically significant reduced when compared to the control (Two
sample t-test procedure). The test material at the rate of 100 mg/L had no toxic effects on respiration activity of activated sludge.
Therefore it can be assumed that the NOEC is above 100 mg test item/L. - Results with reference substance (positive control):
- 3,5-Dichlorophenol
EC50 = 8.1 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- At a nominal test concentration of 100 mg test item/L less than 10% inhibition was observed after three hours of incubation. The mean deviation in respiration rate was 4.2% at three replicates of 100 mg test material.
The respiration rate of the activated sludge to the test material at the rate of 100 mg/L was not statistically significant reduced when compared to the control (Two sample t-test procedure). The test material at the rate of 100 mg/L had no toxic effects on respiration activity of activated sludge. Therefore it can be assumed that the NOEC is above 100 mg test item/L. - Executive summary:
Purpose
The purpose of the 3-hour toxicity test was to evaluate the influence of the test item on the activity of activated sludge by measuring the respiration rate under defined conditions. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of various concentrations of the test item after an incubation period of 3 hours.
Study Design
The study was performed according to OECD Guideline 209, Activated Sludge, Respiration and Inhibition Test (Carbon and Ammonium Oxidation), adopted 22 October 2010.
The test item was directly dosed into each test flask and pure water was added. The test item was dissolved into the pure water as homogeneously as possible.
Test Concentrations
100 mg test item (nominal);
1, 4 and 16 mg 3,5 -Dichlorophenol/L (positive control) and
2 inoculum controls.
Results
Toxicity of Test Item
At a nominal test concentration of 100 mg test item/L less than 10% inhibition was observed after three hours of incubation. The mean deviation in respiration rate was 4.2% at three replicates of 100 mg test material
The respiration rate of the activated sludge to the test material at the rate of 100 mg/L was not statistically significant reduced when compared to the control (Two sample t-test procedure). The test material at the rate of 100 mg/L had no toxic effects on respiration activity of activated sludge. Therefore it can be assumed that the NOEC is above 100 mg test item/L.
Toxicity of 3.5 -Dichlorophenol
The positive control 3,5-Dichlorophenol was tested in the same way as the test item. The 3-hour EC50 for total respiration was found to be 8.7 mg/L thus lying in the range of 5 - 30 mg/L recommended by the test guidelines. The result confirms the suitability of the activated sludge used.
Reference
Description of key information
The test item had no inhibiting effects on the respiration rate of activated sludge up to and including 100 mg/L.
The NOEC was determined to be > 100 mg /L. The 3-hour EC10, EC20, and EC50 values could not be established.
Key value for chemical safety assessment
Additional information
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