Registration Dossier
Registration Dossier
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EC number: 603-493-6 | CAS number: 131560-11-7
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- triphenyl[(2E,4E,6E)-3,7,11-trimethyldodeca-2,4,6,10-tetraen-1-yl]-λ⁵-phosphane
- EC Number:
- 603-493-6
- Cas Number:
- 131560-11-7
- Molecular formula:
- C33 H38 P
- IUPAC Name:
- triphenyl[(2E,4E,6E)-3,7,11-trimethyldodeca-2,4,6,10-tetraen-1-yl]-λ⁵-phosphane
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Inoculum: Activated sludge from laboratory waste water plants fed with municipal sewage
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 10 mg/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD 301 B
- Additional substrate: no
- Solubilising agent (type and concentration if used):
- Test temperature: room temperature
- pH: 7.7; 7.4 after adjustment
- pH adjusted: yes
- Aeration of dilution water: yes
- Inoculum:6 g/L dry matter
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: brwon glass flasks
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: areation
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: KOH trap
SAMPLING
- Sampling frequency: various
- Sterility check if applicable: no
- Sample storage before analysis: no
CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Abiotic sterile control: 1
- Toxicity control: 1
STATISTICAL METHODS:
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 10
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- day 3: < 10 %
day 5: 30-40 %
day 14: 70-80%
day 28: 80-90%
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: poorly biodegradable
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