Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 840-202-3 | CAS number: 2101947-22-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The study was conducted between 01 February 2018 and 27 March 2018.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted according to OECD 209 guidelines and in compliance with GLP without deviations that influence the result.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identification: FRET 15-0735
Physical state/Appearance: clear colorless liquid
Storage Conditions: room temperature in the dark - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Range-Finding Test:
The test concentrations to be used in the definitive test were determined by a preliminary range finding test.
In the range finding test, activated sewage sludge micro organisms were exposed to a series of nominal test concentrations of 10, 100 and 1000 mg/L. The test item was dispersed directly in water.
Nominal amounts of test item (5, 50 and 500 mg (500 mg in triplicate)) were each separately dispersed in approximately 200 mL of deionized reverse osmosis water and subjected to ultrasonication for approximately 15 minutes followed by magnetic stirring for 24 hours, at room temperature, in order to maximize the dissolved test item concentration. All test vessels were shielded from the light during mixing. Synthetic sewage (16 mL), activated sewage sludge (250 mL) and water were added to a final volume of 500 mL to give the required concentrations of 10, 100 and 1000 mg/L (three replicates at 1000 mg/L).
The pH of the test item dispersions was measured after stirring using Hach HQ40d Flexi handheld meter and if necessary, adjusted to between pH 7.0 and 8.0.
The control group was maintained under identical conditions but not exposed to the test item.
Definitive Test:
The test item was dispersed directly in water.
Nominal amounts of test item (50, 90, 160, 280 and 500 mg (three replicates of each)) were each separately dispersed in approximately 200 mL of deionized reverse osmosis water and subjected to ultrasonication for approximately 15 minutes followed by magnetic stirring for 24 hours, at room temperature, in order to maximize the dissolved test item concentration. All test vessels were shielded from the light during mixing. Synthetic sewage (16 mL), activated sewage sludge (250 mL) and water were added to a final volume of 500 mL to give the required concentrations of 100, 180, 320, 560 and 1000 mg/L (three replicates of each).
The pH of the test item dispersions was measured after stirring using Hach HQ40d Flexi handheld meter and if necessary, adjusted to between pH 7.0 and 8.0.
As it was not a requirement of the Test Guidelines, no analysis was conducted to determine the homogeneity, concentration or stability of the test item formulation. This is an exception with regard to GLP and has been reflected in the GLP compliance statement.
The control group was maintained under identical conditions but not exposed to the test item. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Inoculum
A mixed population of activated sewage sludge micro-organisms was obtained on 1 February 2018 for the range finding test and on 26 March 2018 for the definitive test from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK which treats predominantly domestic sewage.
Preparation
The activated sewage sludge sample was maintained on continuous aeration in the laboratory at a temperature of approximately 21 ºC overnight prior to use in the test. On the day of collection the activated sewage sludge (9.5 liters) was fed synthetic sewage (475 mL). The pH of the sample on the day of the test was 7.4 measured using a Hach HQ40d Flexi handheld meter. Determination of the suspended solids level of the activated sewage sludge was carried out by filtering a sample (100 mL) of the activated sewage sludge by suction through a pre-weighed GF/A filter paper* using a Buchner funnel which was then rinsed three times with 10 mL of deionized reverse osmosis water and filtration continued for 3 minutes. The filter paper was then dried in an oven at approximately 105 ºC for at least 1-Hour and allowed to cool before weighing. This process was repeated until a constant weight was attained. The suspended solids concentration was equal to 3.0 g/L prior to use.
* Rinsed 3 times with 20 mL deionized reverse osmosis water prior to drying in an oven
Test water:
The test water used for the range finding and definitive tests was deionized reverse osmosis water containing less than 1 mg/L Total Organic Carbon (TOC).
SYNTHETIC SEWAGE
A synthetic sewage of the following composition, was added to each test vessel to act as a respiratory substrate:
16 g Peptone
11 g Meat extract
3 g Urea
0.7 g NaCl
0.4 g CaCl2.2H2O
0.2 g MgSO4.7H2O
2.8 g K2HPO4
Each constituent was dissolved in a final volume of 1 liter of water with the aid of ultrasonication.
The pH of the synthetic sewage stock used to feed the activated sewage sludge and used in the range finding and definitive tests was between pH 7.2 and 7.3. The pH values were measured using a Hach HQ40d Flexi handheld meter. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 °C
- pH:
- pH 7.0-8.0
- Nominal and measured concentrations:
- Test substance
Range-Finding Test: Nominal: 10, 100 and 1000 mg/l.
Definitive Test: 100, 180, 320, 560 and 1000 mg/l.
Reference substance
Range-Finding Test: 3.2, 10 and 32 mg/l.
Definitive Test: 3.2, 10 and 32 mg/l. - Details on test conditions:
- At time "0" 16 mL of synthetic sewage was diluted to 250 mL with water and 250 mL of inoculum added in a 500 mL conical flask (first control). The mixture was aerated with clean, oil-free compressed air via narrow bore glass tubes at a rate of 0.5 to 1.0 liter per minute. Thereafter, at 15 minute intervals the procedure was repeated for the second control followed by the reference item vessels with appropriate amounts of the reference item. Two additional control vessels were then prepared prior to the test item vessels being prepared. Finally, two further control vessels were prepared.
The test was conducted under normal laboratory lighting in a temperature controlled room at measured temperatures of approximately 20 °C. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (C6H3CI2.OH), Purity 97%
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 570 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Range-Finding Test
The dissolved oxygen concentrations after 30 minutes contact time in all vessels were above 60% of the dissolved oxygen saturation level of 8.9 mg O2/L.
No statistically significant toxic effects were shown at the test concentrations of 10 and 100 mg/L, however statistically significant toxic effects were shown at the test concentration of 1000 mg/L.
Based on these results and after discussion with the Sponsor, it was considered necessary to perform a definitive test in order to obtain an EC50 value for the test item. Test concentrations of 100, 180, 320, 560 and 1000 mg/L were selected for the definitive test.
Validation Criteria
• The EC50 value (3-Hour contact time) for the reference item, 3,5-Dichlorophenol, was 7.9 mg/L.
• The specific respiration rate of the controls was 27.33 mg oxygen per gram dry weight of sludge per hour.
• The coefficient of variation of oxygen uptake in the control vessels was 11%.
All validation criteria for the study were therefore satisfied.
Inhibition of Respiration Rate:
In some instances, the initial and final dissolved oxygen concentrations were outside those recommended in the test guidelines (7 mg O2/L and 2 mg O2/L respectively). This was considered to have had no adverse effect on the results of the study given that in all cases the oxygen consumption rate was determined over the linear portion of the oxygen consumption trace.
The dissolved oxygen concentrations after 30 minutes contact time in all vessels were above 60% of the dissolved oxygen saturation level of 8.9 mg O2/L. - Results with reference substance (positive control):
- 3,5-dichlorophenol EC50 (3 h): 7.9 mg/I
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (respiration inhibition) values of the substance is on activated sewage sludge was 570 mg/l after 3 hours contact time.
- Executive summary:
A study according to OECD 209 was performed to assess the inhibitory effect of Substance FRET 15-0735 on the respiration of activated sewage sludge. Cultures of activated sewage sludge were incubated with synthetic sewage under vigorous aeration and in the presence of the test substance. Following a preliminary range-finding test, activated sewage sludge was exposed to an aqueous dispersion of the test item at concentrations of 100, 180, 320, 560 and 1000 mg/L (three replicates) for a period of 3 hours at measured temperatures of approximately 20 °C with the addition of a synthetic sewage as a respiratory substrate. A positive control i.e. 3,5-dichlorophenol was tested concurrently with the test substance in order to demonstrate the satisfactory performance of the procedure. The EC50 (respiration inhibition) values of the substance is on activated sewage sludge was 570 mg/l after 3 hours contact time.
Reference
Dissolved Oxygen Concentrations of the Test Preparations after 30 Minutes Contact Time in the Range-Finding Test
Nominal Concentration |
Dissolved Oxygen Concentration |
Dissolved Oxygen Saturation Level of 8.9 mg O2/L (%) |
|
Control |
R1 |
6.60 |
74 |
R2 |
6.53 |
73 |
|
R3 |
5.93 |
67 |
|
R4 |
5.96 |
67 |
|
Test Item |
10 |
5.86 |
66 |
100 |
5.82 |
65 |
|
1000 R1 |
6.34 |
71 |
|
1000 R2 |
7.10 |
80 |
|
1000 R3 |
7.14 |
80 |
|
3,5-Dichlorophenol |
3.2 |
6.92 |
78 |
10 |
7.36 |
83 |
|
32 |
7.73 |
87 |
Oxygen Consumption Rates and Percentage Inhibition Values after 3 Hours Contact Time in the Range-Finding Test
Nominal Concentration |
Initial O2 |
Measurement Period |
Final O2 Reading |
O2 Consumption Rates |
Inhibition |
|
Control |
R1 |
5.0 |
4 |
2.0 |
45.00 |
- |
R2 |
4.5 |
3 |
2.3 |
44.00 |
- |
|
R3 |
4.4 |
3 |
2.2 |
44.00 |
- |
|
R4 |
4.6 |
4 |
1.8 |
42.00 |
- |
|
Test Item |
10 |
3.8 |
3 |
1.5 |
46.00 |
[5] |
100 |
2.8 |
1 |
2.0 |
48.00 |
[10] |
|
1000 R1 |
7.3 |
10 |
5.4 |
11.40 |
74 |
|
1000 R2 |
7.4 |
10 |
5.8 |
9.60 |
78 |
|
1000 R3 |
7.4 |
10 |
5.7 |
10.20 |
77 |
|
3,5-Dichlorophenol |
3.2 |
5.9 |
6 |
2.4 |
35.00 |
20 |
10 |
7.0 |
10 |
3.6 |
20.40 |
53 |
|
32 |
7.8 |
10 |
6.8 |
6.00 |
86 |
R = Replicate
- = Not applicable
[ ] = Increase in respiration rate as compared to controls
pH Values of the Test Preparations at the Start and End of the Exposure Period in the Range-Finding Test
Nominal Concentration |
pH |
||
0 Hours |
3 Hours |
||
Control |
R1 |
7.7 |
7.3 |
R2 |
7.4 |
7.2 |
|
R3 |
7.5 |
7.2 |
|
R4 |
7.5 |
7.3 |
|
Test Item |
10 |
7.4 |
7.2 |
100 |
7.6 |
7.3 |
|
1000 R1 |
7.5 |
7.4 |
|
1000 R2 |
7.5 |
7.5 |
|
1000 R3 |
7.5 |
7.5 |
|
3,5-Dichlorophenol |
3.2 |
7.6 |
7.7 |
10 |
7.9 |
7.9 |
|
32 |
7.6 |
7.6 |
R = Replicate
Observations on the Test Preparations throughout the Test Period in the Range-Finding Test
Nominal Concentration |
Observations on Test Preparations |
|||
0 Hours* |
30 Minutes |
3 Hours |
||
Control |
R1 |
Pale yellow/brown dispersion |
Dark brown dispersion |
Dark brown dispersion |
R2 |
Pale yellow/brown dispersion |
Dark brown dispersion |
Dark brown dispersion |
|
R3 |
Pale yellow/brown dispersion |
Dark brown dispersion |
Dark brown dispersion |
|
R4 |
Pale yellow/brown dispersion |
Dark brown dispersion |
Dark brown dispersion |
|
Test Item |
10 |
Pale yellow/brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
100 |
Pale yellow/brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
|
1000 R1 |
Pale yellow/brown dispersion with oily globules of test item visible dispersed throughout, at the surface and settled on the bottom of the vessel |
Dark brown dispersion with oily globules of test item visible dispersed throughout and at the surface |
Dark brown dispersion with oily globules of test item visible dispersed throughout and at the surface |
|
1000 R2 |
Pale yellow/brown dispersion with oily globules of test item visible dispersed throughout, at the surface and settled on the bottom of the vessel |
Dark brown dispersion with oily globules of test item visible dispersed throughout and at the surface |
Dark brown dispersion with oily globules of test item visible dispersed throughout and at the surface |
|
1000 R3 |
Pale yellow/brown dispersion with oily globules of test item visible dispersed throughout, at the surface and settled on the bottom of the vessel |
Dark brown dispersion with oily globules of test item visible dispersed throughout and at the surface |
Dark brown dispersion with oily globules of test item visible dispersed throughout and at the surface |
|
3,5-Dichlorophenol |
3.2 |
Pale yellow/brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
10 |
Pale yellow/brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
|
32 |
Pale yellow/brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
*Observations made prior to the addition of activated sewage sludge
R = Replicate
pH Values of the Test Item Preparations after Stirring and Prior to the Addition of Inoculum in the Definitive Test
Nominal Concentration |
pH |
||
Prior to Adjustment |
After Adjustment |
||
Test Item |
100R1 |
7.7 |
- |
100R2 |
7.3 |
- |
|
100R3 |
7.5 |
- |
|
180R1 |
7.7 |
- |
|
180R2 |
7.3 |
- |
|
180R3 |
7.5 |
- |
|
320R1 |
7.5 |
- |
|
320R2 |
7.5 |
- |
|
320R3 |
7.2 |
- |
|
560R1 |
7.0 |
7.5 |
|
560 R2 |
6.3 |
7.2 |
|
560 R3 |
6.5 |
7.2 |
|
1000 R1 |
6.5 |
7.2 |
|
1000 R2 |
6.7 |
7.4 |
|
1000 R3 |
6.6 |
7.4 |
R = Replicate
- = No adjustment required
Dissolved Oxygen Concentrations of the Test Preparations after 30 Minutes Contact Time in the Definitive Test
Nominal Concentration |
Dissolved Oxygen Concentration |
Dissolved Oxygen Saturation Level of 8.9 mg O2/L (%) |
|
Control |
R1 |
7.33 |
82 |
R2 |
7.46 |
84 |
|
R3 |
6.88 |
77 |
|
R4 |
6.70 |
75 |
|
R5 |
7.31 |
82 |
|
R6 |
6.25 |
70 |
|
Test Item |
100R1 |
7.42 |
83 |
100R2 |
7.34 |
82 |
|
100R3 |
6.98 |
78 |
|
180R1 |
5.88 |
66 |
|
180R2 |
6.14 |
69 |
|
180R3 |
6.64 |
75 |
|
320R1 |
7.03 |
79 |
|
320R2 |
7.11 |
80 |
|
320R3 |
5.97 |
67 |
|
560R1 |
6.52 |
73 |
|
560R2 |
6.33 |
71 |
|
560R3 |
5.79 |
65 |
|
1000R1 |
6.07 |
68 |
|
1000R2 |
6.80 |
76 |
|
1000R3 |
7.06 |
79 |
|
3,5-Dichlorophenol |
3.2 |
7.50 |
84 |
10 |
7.62 |
86 |
|
32 |
8.34 |
94 |
R = Replicate
Oxygen Consumption Rates and Percentage Inhibition Values after 3 Hours Contact Time in the Definitive Test
Nominal |
Initial O2 |
Measurement Period |
Final O2Reading |
O2Consumption Rates |
Inhibition |
|
Control |
R1 |
3.1 |
2 |
1.9 |
36.00 |
- |
R2 |
4.0 |
3 |
2.0 |
40.00 |
- |
|
R3 |
4.8 |
5 |
1.8 |
36.00 |
- |
|
R4 |
4.2 |
3 |
2.0 |
44.00 |
- |
|
R5 |
4.4 |
3 |
2.3 |
42.00 |
- |
|
R6 |
4.6 |
2 |
3.0 |
48.00 |
- |
|
Test Item |
100R1 |
3.5 |
2 |
2.1 |
42.00 |
[2] |
100R2 |
4.0 |
3 |
2.0 |
40.00 |
2 |
|
100R3 |
4.0 |
4 |
2.0 |
30.00 |
27* |
|
180R1 |
4.2 |
4 |
2.0 |
33.00 |
20 |
|
180R2 |
5.7 |
4 |
3.8 |
28.50 |
30 |
|
180R3 |
3.3 |
2 |
2.8 |
15.00 |
63* |
|
320R1 |
3.7 |
3 |
2.1 |
32.00 |
22 |
|
320R2 |
3.7 |
2 |
2.9 |
24.00 |
41* |
|
320R3 |
4.7 |
5 |
2.0 |
32.40 |
21 |
|
560R1 |
5.5 |
9 |
2.2 |
22.00 |
46 |
|
560 R2 |
6.1 |
10 |
2.8 |
19.80 |
52 |
|
560 R3 |
6.0 |
10 |
2.5 |
21.00 |
49 |
Oxygen Consumption Rates and Percentage Inhibition Values after 3 Hours Contact Time in the Definitive Test
Nominal Concentration |
Initial O2 |
Measurement Period |
Final O2 Reading (mg O2/L) |
O2 Consumption Rates |
Inhibition |
|
Test Item |
1000R1 |
7.5 |
10 |
6.0 |
9.00 |
78 |
1000R2 |
7.5 |
10 |
6.0 |
9.00 |
78 |
|
1000R3 |
7.3 |
10 |
5.8 |
9.00 |
78 |
|
3,5-Dichlorophenol |
3.2 |
5.0 |
6 |
2.0 |
30.00 |
27 |
10 |
6.2 |
10 |
3.2 |
18.00 |
56 |
|
32 |
6.9 |
10 |
5.9 |
6.00 |
85 |
R = Replicate
- = Not appliciable
[ ] =Increase in respiration rate as compared to controls
*Results omitted from calculation of the EC50value as results considered to be erroneous
pH Values of the Test Preparations at the Start and End of the Exposure Period in the Definitive Test
Nominal Concentration |
pH |
||
0 Hours |
3 Hours |
||
Control |
R1 |
7.8 |
7.4 |
R2 |
7.5 |
7.3 |
|
R3 |
7.4 |
7.4 |
|
R4 |
7.5 |
7.3 |
|
R5 |
7.6 |
7.5 |
|
R6 |
7.6 |
7.5 |
|
Test Item |
100R1 |
7.4 |
7.3 |
100R2 |
7.7 |
7.4 |
|
100R3 |
7.5 |
7.4 |
|
180R1 |
7.6 |
7.3 |
|
180R2 |
7.6 |
7.3 |
|
180R3 |
7.4 |
7.3 |
|
320R1 |
7.5 |
7.3 |
|
320R2 |
7.7 |
7.5 |
|
320R3 |
7.9 |
7.5 |
pH Values of the Test Preparations at the Start and End of the Exposure Period in the Definitive Test
Nominal Concentration |
pH |
||
0 Hours |
3 Hours |
||
Test Item |
560R1 |
7.5 |
7.5 |
560R2 |
7.5 |
7.5 |
|
560R3 |
7.4 |
7.4 |
|
1000R1 |
7.6 |
7.7 |
|
1000R2 |
7.5 |
7.7 |
|
1000R3 |
7.6 |
7.6 |
|
3,5-Dichlorophenol |
3.2 |
7.7 |
7.7 |
10 |
7.8 |
7.8 |
|
32 |
7.6 |
7.7 |
R = Replicate
Observations on the Test Preparations throughout the Test Period in the Definitive Test
Nominal Concentration |
Observations on Test Preparations |
|||
0 Hours* |
30 Minutes |
3 Hours |
||
Control |
R1 |
Pale yellow/brown dispersion |
Dark brown dispersion |
Dark brown dispersion |
R2 |
Pale yellow/brown dispersion |
Dark brown dispersion |
Dark brown dispersion |
|
R3 |
Pale yellow/brown dispersion |
Dark brown dispersion |
Dark brown dispersion |
|
R4 |
Pale yellow/brown dispersion |
Dark brown dispersion |
Dark brown dispersion |
|
R5 |
Pale yellow/brown dispersion |
Dark brown dispersion |
Dark brown dispersion |
|
R6 |
Pale yellow/brown dispersion |
Dark brown dispersion |
Dark brown dispersion |
|
Test Item |
100R1 |
Pale yellow/brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
100R2 |
Pale yellow/brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
|
100R3 |
Pale yellow/brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
|
180R1 |
Pale yellow/brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
|
180R2 |
Pale yellow/brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
|
180R3 |
Pale yellow/brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Observations on the Test Preparations throughout the Test Period in the Definitive Test
Nominal Concentration |
Observations on Test Preparations |
|||
0 Hours* |
30 Minutes |
3 Hours |
||
Test Item |
320R1 |
Pale yellow/brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
320R2 |
Pale yellow/brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
|
320R3 |
Pale yellow/brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
Dark brown dispersion, no undissolved test item visible |
|
560R1 |
Pale yellow/brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
|
560R2 |
Pale yellow/brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
|
560R3 |
Pale yellow/brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
|
1000R1 |
Pale yellow/brown cloudy dispersion, with oily of test item visible at the surface, dispersed throughout and settled at the bottom of the vessel |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
|
1000R2 |
Pale yellow/brown cloudy dispersion, with oily of test item visible at the surface, dispersed throughout and settled at the bottom of the vessel |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
|
1000R3 |
Pale yellow/brown cloudy dispersion, with oily of test item visible at the surface, dispersed throughout and settled at the bottom of the vessel |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
Dark brown dispersion, oily specks of test item visible at the surface and small oily globules of test item visible dispersed throughout |
Observations on the Test Preparations throughout the Test Period in the Definitive Test
Nominal |
Observations on Test Preparations |
|||
0 Hours* |
30 Minutes |
3 Hours |
||
3,5-Dichlorophenol |
3.2 |
Pale yellow/brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
10 |
Pale yellow/brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
|
32 |
Pale yellow/brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
Dark brown dispersion, no undissolved reference item visible |
*Observations made prior to the addition of activated sewage sludge
R = Replicate
Inhibition of Respiration Rate
The following results were derived:
|
FRET 15-0735 |
3,5-Dichlorophenol |
||
ECx(3 Hours) |
95% Confidence Limits (mg/L) |
ECx (3 Hours) |
95% Confidence Limits (mg/L) |
|
EC10 |
140 |
- |
1.6 |
- |
EC20 |
230 |
- |
2.4 |
- |
EC50 |
570 |
500 - 640 |
7.9 |
6.1 - 10 |
EC80 |
1000 |
- |
26 |
- |
Description of key information
A study according to OECD 209 was performed to assess the inhibitory effect of Substance FRET 15-0735 on the respiration of activated sewage sludge. Cultures of activated sewage sludge were incubated with synthetic sewage under vigorous aeration and in the presence of the test substance. Following a preliminary range-finding test, activated sewage sludge was exposed to an aqueous dispersion of the test item at concentrations of 100, 180, 320, 560 and 1000 mg/L (three replicates) for a period of 3 hours at measured temperatures of approximately 20 °C with the addition of a synthetic sewage as a respiratory substrate. A positive control i.e. 3,5-dichlorophenol was tested concurrently with the test substance in order to demonstrate the satisfactory performance of the procedure. The EC50 (respiration inhibition) values of the substance is on activated sewage sludge was 570 mg/l after 3 hours contact time.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 570 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.