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EC number: 617-769-9 | CAS number: 858956-08-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Arbeit, Integration und Soziales des Landes Nordrhein-Westfalen, Düsseldorf, Germany
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Pretreatment (of test without ATU): test item was added into Erlenmeyer flasks (incubation vessels) to about 130 mL deionised water and was stirred before testing (equilibration phase) overnight for 17 hours. The pH (3.7 - 3.8) was adjusted to pH 7.1 - 7.5 with NaOH. For the reference compound a stock solution at a concentration of 500 mg/L was prepared by dissolving 250 mg 3,5-Dichlorophenol in 5 mL of 1 N NaOH and diluting to 0.5 litre with deionised water. The pH was adjusted to pH 7 - 8 with HCl.
- Pretreatment (of test with ATU): test item was added into Erlenmeyer flasks (incubation vessels) to about 130 mL deionised water and was stirred before testing (equilibration phase) overnight for 17 hours. The pH (3.7 - 3.8) was adjusted to pH 7.2 - 7.3 with NaOH. For the ATU-solution 2.32 g N-allylthiourea were diluted with deionized water to 1 litre. 1.25 mL of the solution were given to all replicates for the determination of the heterotrophic oxidation immediately before start of the incubation period. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Municipal WWTP Odenthal
, Germany
- Method of cultivation: aeration of the activated sludge at 20 ± 2 °C, daily fed with synthetic medium
- Preparation of inoculum for exposure: sludge was settled and the supernatant was decanted. After centrifuging the sludge (15 min at 3500 rpm and 20°C) the supernatantwas decanted again. The calculated amount of sludge was dissolved in
synthetic medium and then filled up to a defined end volume with deionised water.
- Initial biomass concentration: 3 g/L dw suspended solids. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 18.6 - 19.2°C
- pH:
- 7.9 - 8.6
- Nominal and measured concentrations:
- Nominal test substance concentrations: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL glass Erlenmeyer flasks
- Type: closed
- Fill volume: 250 mL
- Aeration: yes (50-100 L/h with clean oil-free air)
- No. of vessels per concentration (replicates): 3 replicates without ATU, 2 replicates with ATU
- No. of vessels per control (replicates): 6 replicates without ATU, 4 replicates with ATU
- No. of vessels per abiotic control (replicates): 1
- Sludge concentration: 1000 mg/L suspended solids
- Nutrients provided for bacteria: synthetic waste water feed
- Nitrification inhibitor used: N-allylthiourea
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Details on termination of incubation: content of the Erlenmeyer flasks was completely transferred to 250 mL BOD bottles
EFFECT PARAMETERS MEASURED: oxygen content (after 3 h) - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol: 2.5, 5, 10, 20 and 40 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50: 12.801 mg/L - Reported statistics and error estimates:
- STUDENT-t test for Homogeneous Variances: Pair-wise comparison of treatments with "Control" by the t test procedure. Significance was Alpha = 0.05. onesided smaller; the differences are significant in case p(i) <= Alpha ; p(F): probability of F computed by a F-test (Ho: var1 = var2 (homogeneity); p(F) > 0.05 is the criterion of variance homogeneity. (The residual variance of an ANOVA was applied; df = N - k; N: sum of treatment replicates n(i); k: number of treatments).
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
Reference
Table 1: Total respiration (without ATU) rates after 3 hours incubation period, percentage inhibition, temperature and pH values
Treatment [mg/L] |
Respiration rate |
Mean Temp. |
pH- |
Inhibition |
|
[mg/L · h] |
[°C] |
value |
[%] |
||
Control 1 |
-- |
38.167 |
19.7 |
8.4 |
-- |
Control 2 |
-- |
34.138 |
19.1 |
8.5 |
-- |
Control 3 |
-- |
34.337 |
19.2 |
8.4 |
-- |
Control 4 |
-- |
34.694 |
19.0 |
8.5 |
-- |
Control 5 |
-- |
35.333 |
18.9 |
8.5 |
-- |
Control 6 |
-- |
30.213 |
19.0 |
8.6 |
-- |
Control, mean |
34.480 |
-- |
-- |
-- |
|
Test item |
100 |
36.233 |
18.9 |
8.6 |
0.000 |
Test item |
100 |
33.893 |
18.6 |
8.6 |
1.705 |
Test item |
100 |
34.428 |
18.7 |
8.6 |
0.151 |
Test item, mean |
100 |
34.851 |
-- |
-- |
0.619 |
Physico-chemical oxygen consumption control |
100 |
0.233 |
18.6 |
7.9 |
-- |
Reference compound |
2.5 |
33.535 |
19.0 |
8.5 |
2.742 |
Reference compound |
5 |
34.710 |
19.2 |
8.5 |
0.000 |
Reference compound |
10 |
18.460 |
19.1 |
8.5 |
46.461 |
Reference compound |
20 |
9.574 |
18.6 |
8.5 |
72.232 |
Reference compound |
40 |
5.766 |
18.8 |
8.5 |
83.277 |
Table 2: Heterotrophic respiration (with ATU) rates after 3 hours incubation period, percentage inhibition, temperature and pH values
Treatment [mg/L] |
Respiration rate |
Mean Temp. |
pH- |
Inhibition |
|
[mg/L · h] |
[°C] |
value |
[%] |
||
Control 1 |
|
31.534 |
19.0 |
8.5 |
-- |
Control 2 |
|
34.068 |
19.0 |
8.5 |
-- |
Control 3 |
|
35.289 |
18.9 |
8.6 |
-- |
Control 4 |
|
37.250 |
19.1 |
8.6 |
-- |
Control, mean |
34.535 |
-- |
-- |
-- |
|
Test item |
100 |
32.693 |
18.9 |
8.6 |
5.336 |
Test item |
100 |
32.895 |
19.0 |
8.6 |
4.749 |
Test item, mean |
100 |
32.794 |
-- |
-- |
5.043 |
Table 3: Validity criteria for OECD 209.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
34.480 mg oxygen per one gram of |
yes |
The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. |
7.4 % |
yes |
Description of key information
NOEC (3 h) ≥ 100 mg/L (nominal, OECD 209, activated sludge respiration rate)
EC50 (3 h) > 100 mg/L (nominal, OECD 209, activated sludge respiration rate)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
One GLP study on the toxicity of the test substance to microorganisms is available.
This key study was performed according to OECD guideline 209 with activated sludge from domestic sewage treatment plant. The activated sludge was exposed to the test substance at a limit test item concentration of 100 mg/L. To determine the heterotrophic oxidation, additional controls and exposure groups (test item concentration of 100 mg/L), all containing 1.25 mL of specific nitrification inhibitor, ATU-solution (N-allylthiourea), were tested.
The respiration rate of each mixture was determined after aeration periods of 3 hours. As no significant inhibitory effect was measured at a limit test item concentration of 100 mg/L no statistical analysis was required to determine the EC50. The NOEC (3 h) was calculated to be the test concentration of 100 mg/L.
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