4-propylcyclohexanone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1984

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: Strauss
- Age at study initiation: less than 24 h old
- Source: Not indicated
- Feeding during test : No

ACCLIMATION
- Acclimation period: No details available

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

19.1 °d

Test temperature:

21 °C

pH:

7.8

Dissolved oxygen:

88 - 100%

Nominal and measured concentrations:

Nominal: control, 1, 10, 18, 32, 58 and 100 mg/L
No measured concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 mL glass beaker
- Volume of solution: 40 mL
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Standard dilution water according to OECD 202

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 8 hours dark, 16 hours light

EFFECT PARAMETERS MEASURED: Immobilisation of the Daphnia was recorded after 24h and 48h. The pH and oxygen concentration were measured at the beginning and the end of each test.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

33 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: None

Table 1: Cumulative immobilization at 24 h and 48 h, Cumulative percentage immobilization in ( )

Concentration (mg/L)	Immobile Daphnids
	24 h	48 h
100	20 (100)	20 (100)
58	9 (45)	12 (60)
32	2 (10)	10 (50)
18	0 (0)	7 (35)
10	0 (0)	0 (0)
1	0 (0)	0 (0)
0	0 (0)	0 (0)

Validity criteria fulfilled:

yes

Conclusions:

The acute immobilization study with Daphnia magna was performed according to OECD 202. Under the given conditions the 48h EC50 value was 33 mg/L.

Executive summary:

The acute toxicity of the test material was determined in a study according to OECD TG 202. For this purpose, young Daphnia magna were exposed to aqueous test material concentrations under defined conditions. The study comprised of one vessels per concentration containing 20 Daphnia magna, i.e. 20 Daphnia per concentration (test medium group) and control group. The Daphnia were observed for  immobilization/deaths up to 48 hours. Daphnia magna were exposed to the nominal test material concentrations of 1, 10, 18, 32, 58 and 100 mg/L in a static system. The nominal concentrations of 18 to 100 mg/L led to immobilized daphnia. Under the conditions of this study, the 48-h EC50 was determined to be 33 mg/L.

Description of key information

An acute immobilization study with Daphnia magna was performed according to OECD 202. Under the given conditions the 48-h EC50 value was 33 mg/L (reference 6.1.3-1).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

33 mg/L

Additional information

The acute toxicity of the test material was determined in a study according to OECD TG 202. For this purpose, young Daphnia magna were exposed to aqueous test material concentrations under defined conditions. The study comprised of one vessels per concentration containing 20 Daphnia magna, i.e. 20 Daphnia per concentration (test medium group) and control group. The Daphnia were observed for  immobilization/deaths up to 48 hours. Daphnia magna were exposed to the nominal test material concentrations of 1, 10, 18, 32, 58 and 100 mg/L in a static system. The nominal concentrations of 18 to 100 mg/L led to immobilized daphnia. Under the conditions of this study, the 48-h EC50 was determined to be 33 mg/L (reference 6.1.3-1).