4-isocyanato-2-methyl-1-{4-[(trifluoromethyl)thio]phenoxy}benzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April - May 1993

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-isocyanato-2-methyl-1-{4-[(trifluoromethyl)sulfanyl]phenoxy}benzene

EC Number:

690-654-9

Cas Number:

106310-19-4

Molecular formula:

C15H10F3NSO2

IUPAC Name:

4-isocyanato-2-methyl-1-{4-[(trifluoromethyl)sulfanyl]phenoxy}benzene

Test material form:

liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Interfauna U.K. Ltd., Wyton, Huntingdon, England
- Housing: indivudually in stainless steel cages
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: at least 14 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 °C +- 1.5 °C
- Humidity (%): 40% - 70%
- Photoperiod (hrs dark / hrs light): 12
- Air changes (per hr): 12 - 15 times

Test system

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

other: The cotralateral skin area not treated with the test substance served as control

Amount / concentration applied:

500 µg

Duration of treatment / exposure:

4 h

Observation period:

Until the changes observed have completely subsided, however for not more than 14 days after application

Number of animals:

3

Details on study design:

TEST SITE
- Area of exposure: approx. 6 cm²

REMOVAL OF TEST SUBSTANCE
- Washing (if done): with water
- Time after start of exposure: 4 hours

OBSERVATION TIME POINTS
60 minutes, 24, 48, 72 hours, 7 and 14 days after patch removal

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

3

Max. score:

4

Reversibility:

not reversible

Results 2

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

3

Max. score:

4

Reversibility:

not reversible

Results 3

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

2.7

Max. score:

4

Reversibility:

not reversible

Results 4

Irritation parameter:

edema score

Basis:

animal #1

Time point:

24/48/72 h

Score:

2.3

Max. score:

4

Reversibility:

fully reversible within: 14d

Results 5

Irritation parameter:

edema score

Basis:

animal #2

Time point:

24/48/72 h

Score:

1.7

Max. score:

4

Reversibility:

fully reversible within: 14d

Results 6

Irritation parameter:

edema score

Basis:

animal #3

Time point:

24/48/72 h

Score:

1.3

Max. score:

4

Reversibility:

not reversible

Applicant's summary and conclusion

Executive summary:

A single dermal administration of the test substance for 4 hours to rabbits according to OECD TG 404 caused significant irritant effects. In addition to the marked erytherna and oedema formation, on the exposed skin area a white squarnous coat was observed, the skin was hardened and the epiderrnis was destroyed in all three anirnals. Signs did not prove to be reversible up to day 14.