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EC number: 268-706-3 | CAS number: 68133-79-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 12-17 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-(3,7-dimethylocta-2,6-dienyl)cyclopentan-1-one
- EC Number:
- 268-706-3
- EC Name:
- 2-(3,7-dimethylocta-2,6-dienyl)cyclopentan-1-one
- Cas Number:
- 68133-79-9
- Molecular formula:
- C15H24O
- IUPAC Name:
- 2-(3,7-dimethylocta-2,6-dienyl)cyclopentan-1-one
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all
- Sampling method: Samples were taken at test start, 24 hrs, and 48 hrs. 500 uL samples were taken and stabilized with 500 uL acetonitrile.
- Sample storage conditions before analysis: Deep frozen.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance was added to water to create a stock solution. The stock solution was then diluted to create the appropriate concentration. The solution was ultrasonicated for 5 minutes.
- Eluate:
- Differential loading:
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)):
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): The stock solution had an oily layer on the surface.
- Other relevant information:
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain/clone: Daphnia magna Straus, Clone V
- Justification for species other than prescribed by test guideline:
- Age at study initiation (mean and range, SD): less than 24 hrs old
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Stage and instar at study initiation:
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding:
- Source:
- Age of parental stock (mean and range, SD):
- Feeding during test: No
- Food type:
- Amount:
- Frequency:
ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):
QUARANTINE (wild caught)
- Duration:
- Health/mortality:
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS:
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 13 degrees dH
- Test temperature:
- 19.4-20.8 degrees C
- pH:
- 7.69-7.98
- Dissolved oxygen:
- >= 8.4 mg/L
- Nominal and measured concentrations:
- Nominal: 0. 0.640, 0.160, 0.400, 1.00, 2.50 mg/L
Measured: 0, 0.0272, 0.806, 0.155, 0.449, 1.26 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beakers
- Type (delete if not applicable): covered with glass plates
- Material, size, headspace, fill volume: glass, 100 mL
- Volume of solution: 50 mL
- Aeration: No
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 test medium
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hrs light/ 8 hrs dark
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilisation at 24 and 48 hrs
VEHICLE CONTROL PERFORMED: yes/no
RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate at concentrations of 0.60 and 2.10 mg/L
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.677 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities:
At the 24 hr observation, the mobile daphnids appeared sluggish. At the 48 hr observation, half of the daphnids were swimming on the surface at the 1.00 mg/L concentration.
- Observations on body length and weight:
- Other biological observations:
- Mortality of control: None
- Other adverse effects control:
- Immobilisation of control: None
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
- Effect concentrations exceeding solubility of substance in test medium: - Results with reference substance (positive control):
- - Results with reference substance valid?
Yes
- Relevant effect levels: 100% of the daphnids were immobilised at 2.10 mg/L by the 24 hr observations. No animals were immobilised at the 0.60 mg/L concentration. The EC50 is therefore between 0.60 and 2.10 mg/L.
- Limit test: No
- Dose-response test: Yes
- ECx: EC50 0.60-2.10 mg/L
- Other:
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hr EC50 in daphnids is 0.677 mg/L (measured).
- Executive summary:
The toxicity of Apritone to aquatic invertebrates was tested in an OECD guideline 202 study. 20 daphnids were exposed to concentrations of 0, 0.0272, 0.806, 0.155, 0.449, and 1.26 mg/L (measured) for 48 hrs. The reference substance potassium dichromate was also tested. Results with negative and positive controls were valid. The 48 -hr EC50 based on immobilisation was 0.677 mg/L (measured).
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