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EC number: 222-001-7 | CAS number: 3312-60-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 1963
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- study review
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- sex not specified, test substance not removed after 24 h, no statistics, only 3 animals per dose group used, shorter observation period of 7 days
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 3-cyclohexylaminopropylamine
- EC Number:
- 222-001-7
- EC Name:
- 3-cyclohexylaminopropylamine
- Cas Number:
- 3312-60-5
- Molecular formula:
- C9H20N2
- IUPAC Name:
- N1-cyclohexylpropane-1,3-diamine
- Test material form:
- liquid
- Details on test material:
- 3-Cyclohexylaminopropylamine from Performance Chemicals, lot number: 68-219-BC
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- other: Albino
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- Rabbits weighting between 2.0 and 2.8 kg
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- not specified
- Details on dermal exposure:
- Three dose groups were used in the study. All rabbits were prepared for the test by closely clipping the fur over the trunk and each rabbit was given a single application of the test compound. The test material was spread evenly over the back by a glass rod, and the rabbit was then wrapped in gauze and covered by a further wrapping of rubber damming, fastened with adhesive tape. The rabbits were then returned to their cages where food and water were available.
- Duration of exposure:
- After single exposure of test material, the animals were observed for seven days.
- Doses:
- Group 1 2000 mg/kg 3 rabbits
Group 2 632 mg/kg 3 rabbits
Group 3 200 mg/kg 3 rabbits - No. of animals per sex per dose:
- 3 animals per dose
- Control animals:
- no
- Details on study design:
- Three dose groups. All rabbits were prepared for the test by closely clipping the fur over the trunk and each rabbit was given a single application of the test compound. The test material was spread evenly over the back by a glass rod, and the rabbit was then wrapped in gauze and covered by a further wrappingof rubber damming, fastened with adhesive tape.. The rabbits were then returned to their cages where food and water were available.
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- not specified
- Dose descriptor:
- LD100
- Effect level:
- 2 000 mg/kg bw
- Based on:
- test mat.
- Key result
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- > 632 - <= 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- All rabbits in the high dose group died within 28 hours after dosing
- Clinical signs:
- other: Considerably pain in the HD and MD dose group because the animals in these dose groups screamed for several minutes after application if test compound
- Gross pathology:
- Badly damaged skin and necrosis in the MD animals. In dead animals of the HD group: Black thickened skin and internally adhesions of cecum and portions of small intestine to the peritoneal lining of one rabbit receiving 632 mg/kg.
Any other information on results incl. tables
Acute Dermal toxicity for the Rabbit
Rabbit No | Dose in mg/kg | Body weight initial [kg] | Body weight sacrifice [kg] | Day 1 | Day 2 | Day 3 | Day 4 | Day 5 | Day 6 | Day 7 | Autopsy |
1 | 2000 | 2.3 | dead | Compound has burned through all layers of skin and the entire intestinal tract appeared hemorrhagic | |||||||
2 | 2000 | 2.0 | XXX | Pupils dilatated, weak, depressed apperance | |||||||
Erythema | dead | ||||||||||
Edema | |||||||||||
3 | 2000 | 1.9 | dead | Compound has burned through skin | |||||||
4 | 632 | 2.0 | 1.7 | Pupils dilated | |||||||
Erythema | XXX | XXX | XXX | XXX | Black, necrotic | sac | |||||
Edema | XXX | XXX | XXX | XXX | |||||||
5 | 632 | 2.1 | 1.8 | Pupils dilated | |||||||
Erythema | XXX | XXX | XXX | XXX | Black, necrotic | sac | |||||
Edema | XXX | XXX | XXX | XXX | |||||||
6 | 632 | 2.3 | 1.9 | Pupils dilated | |||||||
Erythema | XXX | XXX | XXX | XXX | necrotic | sac | |||||
Edema | XXX | XXX | XXX | XXX | |||||||
7 | 200 | 2.4 | 2.2 | Pupils slightly dilated | |||||||
Erythema | XXX | XXX | XX | XX | necrotic | sac | |||||
Edema | XXX | XXX | XXX | XXX | |||||||
8 | 200 | 2.3 | 2.1 | Pupils slightly dilated | |||||||
Erythema | XXX | XXX | XX | XX | necrotic | sac | |||||
Edema | XXX | XXX | XXX | XX | |||||||
9 | 200 | 2.4 | 2.2 | Pupils slightly dilated | |||||||
Erythema | XXX | XXX | XX | XX | necrotic | sac | |||||
Edema | XXX | XXX | XXX | XX |
Applicant's summary and conclusion
- Conclusions:
- The compound is considered to be a severe skin irritant and possesses the ability to penetrate the skin and produce systemic toxicity and even death in the rabbit.
- Executive summary:
Nine albino rabbits were divided into three dose groups of 200, 632 and 2000 mg/kg. The test material was applied to the clipped skin once and then the rabbit was wrapped in gauze and covered by wrapping of rubber damming. All three rabbits of the high dose died within the first 28 h after dosing. All survivors lost 300 - 400 g in body weight. The skin wad badly damaged in all cases and showed severe necrosis. Post-mortem observations on survivors included: black, thickened skin and internally adhesions of cecum and portions of small intestine to the peritoneal lining of one rabbit in MD.
The compound must be considered as severe skin corrosive and possesses the ability to penetrate the skin and to produce systemic toxicity and the LD 50 value was determined to be between 632 and 2000 mg/kg bw/d.
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