Allyltributylphosphonium chloride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study plan dated : 15 May 2018 and experimental completion date 21 Jun 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Allyltributylphosphonium chloride

EC Number:

216-231-7

EC Name:

Allyltributylphosphonium chloride

Cas Number:

1530-48-9

Molecular formula:

C15H32P.Cl

IUPAC Name:

tributyl(prop-2-en-1-yl)phosphanium chloride

impurity 1

Reference substance name:

Tetrahydrothiophene 1,1-dioxide

EC Number:

204-783-1

EC Name:

Tetrahydrothiophene 1,1-dioxide

Cas Number:

126-33-0

Molecular formula:

C4H8O2S

IUPAC Name:

tetrahydrothiophene 1,1-dioxide

impurity 2

Reference substance name:

Tributyl-1-propen-1-yl-phosphonium chloride

Molecular formula:

C15H32P.Cl

IUPAC Name:

Tributyl-1-propen-1-yl-phosphonium chloride

Test material form:

other: solid : wax like

Details on test material:

Homogeneity : homogeneous if totally melted
Note: For every use the test item was completely melted and the melted test item
was used for the test.

Specific details on test material used for the study:

Designation in Test Facility: 18032901G
Date of Receipt: 29. Mar. 2018
Condition at Receipt: Room temperature, in proper conditions
Batch no. INT 18 192
Appearance Beige crystallised mass
Homogeneity homogeneous if totally melted
Vapour pressure <0.1 hPa at 30 °C
Stability in solvents H2O: unknown; EtOH: unknown; acetone: unknown; CH3CN: unknown; DMSO: unknown
Solubility in solvents H2O: > 1 g/L; EtOH: unknown; acetone: unknown;
CH3CN: unknown; DMSO: unknown
Expiry date 17. Oct. 2019
Storage Room Temperature (20 ± 5°C)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Activated sludge from a biologic sewage treatment plant was used as inoculum. The cho-sen plant treats mostly domestic sewage.
The sludge was taken from the activation basin of the sewage treatment plant, In den Seewiesen, 67480 Edenkoben
The sludge was filtrated through a cloth, washed with test medium (2x) and re-suspended in test medium. It was then aerated until use. The dry matter was determined to contain 4520 mg of suspended solids/L.

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 25 mg/L

Based on:

not specified

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation 1

Parameter followed for biodegradation estimation:

CO2 evolution

Parameter followed for biodegradation estimation 2

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

Apparatus
The test vessels were aerated with purified (by activated charcoal), CO2-scrubbed, mois-tened air. The scrubbing of carbon dioxide was achieved by bubbling the purified air through a flask containing 1.5 M NaOH. To control the absence of CO2, the air was then led through a flask containing a solution of Ba(OH)2 before reaching the test vessels.
Magnetic stirrers were used to prevent deposition of inoculum.
The emitted CO2 was trapped in 0.25 M NaOH. Two scrubbers containing 100 mL each were connected in series to the test vessels. The initial IC value of the 0.25 M NaOH was separately determined in each flask.

Sampling
From each front scrubber flask, 9 samples were taken in order to determine the emitted CO2 (on day 0, 2, 6, 8, 10, 14, 17, 23 and 29). The sample volume was 1 mL. The result-ing change in the volume of the front flask was considered in the calculation of emitted CO2 (see also chapter 8.3.1).
On day 28, 5 mL HCl 2 M was added to each test flask in order to drive off dissolved CO2. On day 29, samples from both scrubber flasks were taken.

CO2 Determination
Analyses of the emitted CO2 were made by IC measurement using the carbon analyser TOC multi N/C 2100S, Analytik Jena. Each sample was measured in duplicate or triplicate, respectively (depending on the variation between the measured values). The carbon ana-lyser was calibrated with freshly prepared reference solutions containing potassium hydro-gen phthalate (TC), sodium hydrogen carbonate and sodium carbonate (IC) every month. After every start, quality control samples were measured.

Reference substance

Reference substance:

aniline

Remarks:

Aniline was chosen as positive control.

Results and discussion

Preliminary study:

not performed

Test performance:

Preparations
The medium was prepared from the stock solutions. The stock solution of the positive con-trol was prepared and its DOC was measured. The stock solution of the test item was pre-pared and its DOC was measured. The inoculum was taken from its source, washed, aer-ated and the dry matter was determined.
The test vessels were filled with medium and inoculum. Then, all flasks were aerated for 96 hours with purified, CO2-free, moistened air to purge the system of CO2.
Sampling
From each front scrubber flask, 9 samples were taken in order to determine the emitted CO2 (on day 0, 2, 6, 8, 10, 14, 17, 23 and 29). The sample volume was 1 mL. The result-ing change in the volume of the front flask was considered in the calculation of emitted CO2 (see also chapter 8.3.1).
On day 28, 5 mL HCl 2 M was added to each test flask in order to drive off dissolved CO2. On day 29, samples from both scrubber flasks were taken

% Degradation

Key result

Parameter:

% degradation (CO2 evolution)

Value:

ca. 1

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The test item Allyltributylphosphonium chloride is considered as “not readily biode-gradable“.
 The degree of biodegradation reached 1 % after 28 days.
 The 10-day-window was not detected
 Because the test item is a mixture, the 10-day window has not to be taken into ac-count. For the test item Allyltributylphosphonium chloride 1 % biodegradation was observed after 28 days. The 10-day window could not be determined. The criterion of reaching 60% of degradation after 28 days was not met. Allyltributylphosphonium chloride is therefore considered as “not readily biodegradable”.
 The abiotic degradation reached 2.5 %.

Executive summary:

The test item Allyltributylphosphonium chloride was tested using a concentration of nomi-nally 20 mg organic carbon/L Allyltributylphosphonium chloride in test medium following OECD 301B and EU-Method C.4-C.

Aniline was chosen as positive control.

Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L). The test was left running for 28 days.

All validity criteria were met. Degradation of the positive control was 61 % after 10 days.

The following data were determined for the test item Allyltributylphosphonium chloride:

10-day-window: not detected degradation at the end of 10-day-window none degradation at the end of the test 1 % pass level following guideline: 60 % at the end of 10-day-window for pure substances

respective 60 % at the end of the test for mixtures

Therefore, when applying the 10-day-window, Allyltributylphosphonium chloride is not readily biodegradable following OECD 301B and EU C.4-C respectively.

Because the test item is a mixture the 10-day-window does not have to be taken into ac-count.

For the test item Allyltributylphosphonium chloride 1 % biodegradation was observed after 28 days. The 10-day window could not be determined. The criterion of reaching 60% of degradation after 28 days is not met. Allyltributylphosphonium chloride is therefore consid-ered as “not readily biodegradable”.