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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2010-08-12 to 2010-12-17
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
other:
Version / remarks:
toxicity control of OECD 301D study
Deviations:
no
Principles of method if other than guideline:
tox control data from OECD 301D study have been used as information source
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test substance was insufficiently soluble to permit addition via aqueous solution. Individually weighed quantities of test substance were added directly to the appropriate test vessels.
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
The inoculum was derived from the secondary effluent of Shanghai Longhua Sewage Plant. The inoculum was pre-conditioned by aerating the secondary effluent, without other treatment or addition, for 5-7 days at the test temperature of 20 + 1 °C. Control microbe counted at the range of
10000-1000000 orgs/L.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
28 d
Test temperature:
20 ± 1 °C
pH:
7.01 - 7.25
Dissolved oxygen:
saturated
Nominal and measured concentrations:
2 mg/L (above water solubility limit)
Reference substance (positive control):
yes
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
> 2 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks on result:
other: equivalent to 1000 mg/L (nominal)
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
> 2 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks on result:
other: equivalent to 1000 mg/L (nominal)
Results with reference substance (positive control):
sodium laurylsulfonate
Validity criteria fulfilled:
yes
Conclusions:
There was no inhibition of the micro organisms observed at water saturation over 28 d.
Executive summary:

The test material was investigate for ready biodegradation. In this OECD 301D study, the toxicity control containing both, test item and reference item 77 % biodegradation was noted within 14 days and 80.5 % biodegradation was determined after 28 days of incubation.


There was no inhibition of the micro organisms observed at water saturation over 28 d.

Description of key information

There was no inhibition of the micro organisms observed at water saturation over 28 d.

Key value for chemical safety assessment

Additional information

The test material was investigate for ready biodegradation. In this OECD 301F study, the toxicity control containing both, test item and reference item 77 % biodegradation was noted within 14 days and 80.5 % biodegradation was determined after 28 days of incubation.


There was no inhibition of the micro organisms observed at water saturation over 28 d.