Copper cyanide

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian cell study: DNA damage and/or repair

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 475 (Mammalian Bone Marrow Chromosome Aberration Test)

GLP compliance:

yes

Type of assay:

mammalian bone marrow chromosome aberration test

Test material

Test material information

Constituent 1

Reference substance name:

2-hydroxy-2-methylpropionitrile

EC Number:

200-909-4

EC Name:

2-hydroxy-2-methylpropionitrile

Cas Number:

75-86-5

Molecular formula:

C4H7NO

IUPAC Name:

2-hydroxy-2-methylpropionitrile

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

No

Duration of treatment / exposure:

Acute exposure

Frequency of treatment:

Single administration

Doses / concentrations

Doses / concentrations 1

Dose / conc.:

0 mg/kg bw (total dose)

Doses / concentrations 2

Dose / conc.:

1.5 mg/kg bw (total dose)

Doses / concentrations 3

Dose / conc.:

5 mg/kg bw (total dose)

Doses / concentrations 4

Dose / conc.:

15 mg/kg bw (total dose)

No. of animals per sex per dose:

6 rats/sex/dose

Control animals:

yes, concurrent vehicle

Positive control(s):

Cyclophosphamide monohydrate

Examinations

Statistics:

The mean mitotic indices, mean modal numbers, percent aberrant cells and the mean number of aberrations per cell for each group were statistically compared using the Kruskal-Wallis nonparametric analysis of variance and nonparametric pairwise group comparisons (KW-ANOVA). Body weight data was analyzed by analysis of covariance (ANCOVA). All tests were one-tailed at the 95% confidence interval (p < .05).

Results and discussion

Test results

Key result

Sex:

male/female

Genotoxicity:

negative

Toxicity:

not specified

Vehicle controls validity:

valid

Positive controls validity:

valid

Additional information on results:

Results show that no statistically significant increases in the frequency of chromosomal aberrations compared to control values were seen for any of the dose levels that were tested with acetone cyanohydrin. No significant differences were seen in the mean modal numbers or mitotic indices of the test groups when compared to control values.

Applicant's summary and conclusion

Conclusions:

The acute oral administration of 1.5. 5 and 15 mg/kg body weight of Acetone Cyanohydrin to male and female rats produced no significant increases in the frequency of chromosomal aberrations. No significant difference was observed between the negative control and test groups when comparing modal numbers or mitotic index. Therefore, under the conditions of this study, Acetone Cyanohydrin is considered not to be clastogenic at any of the levels tested.