Methyl 2-((allyloxy)methyl)acrylate

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 Dec 2013 - 13 Dec 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Sampling method: samples tested at beginning of exposure, 24, 48, and 72 hours
- Sample storage conditions before analysis: frozen

Vehicle:

no

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Strain: ATCC22662
Sourse: American Type Culture Collection
Common name: unicellular green algae

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Hardness:

not specified

Test temperature:

22°C

pH:

7.8

Dissolved oxygen:

not specified

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

Nominal: 0. 0.5, 1.1, 2.4, 5.3, 12.0, 26.0 mg/L
measured: 0.462, 1.04, 2.33, 5.34, 12 and 25.8 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 500mL Erlenmeyer flask with stopper
- Type): closed
- Material, size, headspace, fill volume: headspace 490mL

- Initial cells density: 5x10e3
- No. of vessels per concentration (replicates): 3 per concentration group
- No. of vessels per control (replicates): 6 per control group,

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Light intensity and quality: 60 - 65 µE/m2/s, continuous illumination with whire fluorescent lamp

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]: electronic particle counter, and hemacytometer on a microscope

TEST CONCENTRATIONS
- Range finding study: 1, 10 , 100mg/L
- Test concentrations: 0.5, 1.1, 2.4, 5.3, 12, 26mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

8.72 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

2.33 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: no
- Colour differences: no
- Flocculation: no
- Adherence to test vessels: no
- Aggregation of algal cells: no

Results with reference substance (positive control):

EC50 - 0.780mg/L

Validity criteria fulfilled:

yes

Conclusions:

72 hour EC50: 8.72mg/L
72 hour NOEC: 2.33mg/L

Description of key information

Pseudokirchnerella Subcapitata, 72 hours (growth rates)

EC50 - 7.82 mg/L

NOEC 2.33 - mg/L

Key value for chemical safety assessment

EC50 for freshwater algae:

7.82 mg/L

EC10 or NOEC for freshwater algae:

2.33 mg/L

Additional information

An algal growth inhibition study was conducted (Mitsubishi Chemical Medience Corporation, 2014, Study A130913) to determine the effect of 2-(Allyloxymethyl) acrylic acid methyl ester on the growth of Selenastrum Capricornutum (new name Pseudokirchnerella Subcapitata). The study was conducted according to OECD test guideline 201 and in compliance with GLP.

Triplicate algal cultures were exposed to 2-(Allyloxymethyl) acrylic acid methyl ester at concentrations 0.5, 1.1, 2.4, 5.3, 12.0 and 26 mg/L and incubated under continuous illumination for 72 hours. Six replicate control cultures were also incubated concurrently with the test samples.

Biomass was not affected by treatment with 2-(Allyloxymethyl) acrylic acid methyl ester at the lower concentration groups 0.5 and 1.1 mg/L), so the No Observed Effect level in terms of growth rate was 2.33 mg/L.

The growth inhibition of algae after exposure to 2-(Allyloxymethyl) acrylic acid methyl ester was observed at the higher concentration groups (5.3, 12.0 and 26.0 mg/L).The EC50 in terms of growth rate was found to be 8.72 mg/L.