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EC number: 277-288-1 | CAS number: 73138-39-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 Mar - 09 Apr 2020
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 2019
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control and 100 mg/L (nominal loading)
- Sampling method: samples were analyzed at the beginning and in the end of the test - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Because of the very poorly solubility in water of the test item an extraction has been performed to better solubilize the soluble components of the test item in water, according to OECD Guidance N. 23. After the range finding test the test item has been prepared at 100 mg/L in tap water (saturated solution exceeding the maximum solubility of the test item). The solution has been extracted for 72 h, at 10 rpm, at room temperature. From extract the solution have been prepared understirring. The test has been performed on the nominal solution so obtained.
- Controls: 1 - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Source: Universita’ Milano Bicocca, Univerity of Milan, Milan, Italy
- Length at study initiation (length definition, mean, range and SD): 1-2 cm
ACCLIMATION
- Acclimation period: 11 days for range-finding test, 14 days for definitive test
- Acclimation conditions (same as test or not): yes
- Type and amount of food during acclimation: Feedstuff in flakes and granules
- Feeding frequency during acclimation: 3 times a week with suspension 24 hours before the beginning of the test
- Health during acclimation (any mortality observed): No abnormalities were observed
FEEDING DURING TEST
- Food type: no feeding during test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 40 - 250 mg CaCO3/L
- Test temperature:
- 23.9 - 24.1 °C
- pH:
- 7.60 - 7.83
- Dissolved oxygen:
- 76 - 79% saturation of dissolved oxygen
- Nominal and measured concentrations:
- - nominal: control, 100 mg/L
- measured (0 h): -, 10.26 mg/L
- measured (96 h): -, 10.38 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10L glass tanks
- Type (delete if not applicable): open
- Aeration: continuous with porous stones
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: < 0.8 g/L.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: To reduce the eventual presence of chlorine, the tap water used for the assay has been allowed to stabilize for 48 hours. Tanks have been kept under control for temperature and oxygen for approximately 1 hour before introducing fish.
- Culture medium different from test medium: no
- Intervals of water quality measurement: every 24 hours
OTHER TEST CONDITIONS
- Photoperiod: 12 hours of light and 12 hours of darkness, tanks have been lighted with white light lamps
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Visible abnormalities such as equilibrium loss, irregular swimming, difficulties in respiratory functions and variation of pigmentation have been observed after 2 h + 0.5h and 5 h +1 h from the beginning of the test and twice per day until the end of the test and recorded.
TEST CONCENTRATIONS
- Range finding study : Yes
- Test concentrations: 100, 10, 10, 1, 0.1 mg/L - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10.26 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: No abnormalities were observed during the test
- Sublethal observations / clinical signs:
Table 1: Measured concentrations of the test item
Nominal concentration
Measured concentration 0 h (mg/L)
Measured concentration 96 h (mg/L)
% Stability of measured concentration
100 mg/L
10.26
10.38
101.15
Table 2: Number of dead fish in control and treated groups during the test (n = 7)
2 h
5 h
1 d
1 d
2 d
2 d
3 d
3 d
4 d
4 d
Check No.
-
-
1st
2nd
1st
2nd
1st
2nd
1st
2nd
Control
0
0
0
0
0
0
0
0
0
0
100 mg/L
0
0
0
0
0
0
0
0
0
0
Table 3: Validity criteria for OECD 203.
Criterion from the guideline
Outcome
Validity criterion fulfilled
The mortality in the control(s) should not exceed 10% (or one fish if less than ten are used) at the end of the test
0%
Yes
The dissolved oxygen concentration must have been at least 60 per cent of the air
saturation value throughout the test
76 - 79% air saturation throughout the test
Yes
There must be evidence that the concentration of the substance being tested has been
satisfactorily maintained, and preferably it should be at least 80 per cent of the
nominal concentration throughout the test. If the deviation from the nominal
concentration is greater than 20 per cent, results should be based on the measured
concentration.
The concentration at the end of the test showed a recovery of 101.15% of the initial tested concentration
Yes
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 3 at 'any other informations on results incl. tables'
Reference
Description of key information
LC50 (96 h) > 10.26 mg/L (initial measured concentration) (OECD 203, 2019)
LC50 (96 h) > 100 mg/L (nominal concentration) (OECD 203, 2019)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 10.26 mg/L
Additional information
One GLP study is available, in which the short-term toxicity (96 h) of the UVCB substance fatty acids, coco, esters with 1,3-butanediol (CAS 73138-39-3) to fish was investigated. This study was conducted according to OECD guideline 203 (2019). After a previous range-finding study, the test was conducted as a limit test with a nominal concentration of 100 mg/L. The initial measured concentration was 10.26 mg/L test item, and at the end of the test 10.38 mg/L test item. Thus, the test item was stable over the time of exposure. The results were based on the initial measured concentration. At the end of the test no mortality and no abnormalities were observed in treated and control fish. The 96-h LC50 was determined as > 10.26 mg /L based on the initial measured concentration (LC50 (96 h) > 100 mg/L (nominal)). All validity criteria were met and the test is considered valid.
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