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EC number: 211-402-2 | CAS number: 643-79-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-11-11 to 2002-12-11
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 29 December 1992
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Name: "o-Phthaldialdehyde"
Chemical name: 1,2-Benzene dicarboxyaldehyde
CAS No.: 643-79-8
Molecular formula: C8H6O2
Supplier: DSM Fine Chemicals
Batch No.: OSHO 201
Appearance: Pale yellow scales or powder
Boiling point: 83 °C/1 mbar
Melting range: 54–56 °C
Solubility: in water: 53 g/L at 20 °C; in other solvents: organic solvents
Conditions of storage: Refrigerator, in the dark, may be used under light
Stability at conditions of storage: stable
Expiration date: January 2004 - Analytical monitoring:
- yes
- Vehicle:
- no
- Remarks:
- Reconstituted water
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
Immediately before the beginning of the test a stock solution of 100 mg/L was prepared by dissolving 9.9 mg test substance in 100 mL deionised water. The appropriate concentrations were then obtained by diluting with reconstituted water. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: not specified; neonates less than 24 hours old obtained by hatching ephippia
- Source: Daphtoxkit F (TM) "(Fa. BioMicrotests, Industriezone "De Prijkels", Venacoweg 19, 9810 Nazareth, Belgium), Batch No. of ephippia DM050902
- Age of parental stock (mean and range, SD): not specified
- Feeding during test: none, but fed approx. 2 hours before beginning of the test
- Food type: Suspension of spirulina powder (Fa. MicroBioTests)
- Amount: not specified
- Frequency: not specified
CULTURING
The ephippia were hatched in a petri dish containing reconstituted water in a temperature controlled room at approx. 20–22 °C under continuous illumination of approx. 6,000 Lux. According to Fa. MicroBioTests the largest hatching occurs between 72 and 80 hours and the daphnids were collected, at the latest, 90 hours after initiation of hatching. Approx. 2 hours before beginning of the test, the neonates were fed, as also in traditional cultures the neonates have the opportunity to take up some feed particles. The neonates were collected for test at approx. 87 hours after initiation of hatching. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Checking for immobility after 24 and 48 hours
- Post exposure observation period:
- not specified
- Hardness:
- 250 mg CaCO2/L (reconstituted water)
- Test temperature:
- approx. 21 °C
- pH:
- 7.5-7.9
- Dissolved oxygen:
- 8.3 mg/L
- Salinity:
- not reported
- Conductivity:
- not reported
- Nominal and measured concentrations:
- Nominal: 0.10, 0.18, 0.32, 0.56, 1.00 mg test substance / L in reconstituted water and negative control (reconstituted water only)
Measured (geom. mean): 0.05, 0.07, 0.20, 0.50 and 0.94 mg test substance/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Disposable multiwell test plates with 30 test wells with a transparent lid, provided with the testkit. Each plate has 4 wells (replicates A, B, C, D) for the control and for 5 concentrations. For each concentration, the plates are provided with "rinsing wells" to prevent dilution of the test substance during the transfer of the daphnids from the hatcing petri dish to the test wells. The "rinsing wells" and the wells for the replicates of each concentration and control respectively were filled with 10 mL medium each. For each concentration and for the control at least 20 actively swimming neonates were transferred with a micropipette from the hatching petri dish to the "rinsing well". Then 5 neonates from the "rinsing wells" were transferred to each of the 4 wells of the appropriate concentrations.
- Material, size, headspace, fill volume: 10 mL
- Aeration: No extra aeration, but slight circulation of air is still possible when the lid of the test plate is closed.
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): not applicable
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water according to ISO 6341 from Daphtoxkit F (TM) Batch No. ISO D010802
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours artificial light
- Light intensity: 6,000 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobilisation and mortality checked daily
RANGE-FINDING STUDY
- Test concentrations: A preliminary test with concentrations between 1 and 10 mg/L all daphnids were immobilised in the 1 mg/L concentration
- Results used to determine the conditions for the definitive study: yes. 5 concentrations between 0.1 and 1.0 mg test substance per litre test medium chosen for the test - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.05 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.11 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI: 0.13-0.10
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 0.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.07 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.12 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI: 0.14-0.11
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 0.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Besides immobility and mortality no other effects were reported
- Mortality of control: 0 %
- Other adverse effects control: not reported - Reported statistics and error estimates:
- Not reported
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an acute toxicity test according to the EC 92/69 (1992) Daphnia magna was exposed to 0.05, 0.07, 0.20, 0.50, 0.94 mg/L g ortho-phthalaldehyde/L (meas., geom. mean). The 48-h EC50 of ortho-phthaladehyde to D. magna was 0.11 mg/L (95 %C.I.: 0.10–0.13 mg/L).
- Executive summary:
The 48-hr-acute toxicity of o-phthaladehyde to Daphnia magna was studied under static conditions according to EEC Commission Directive 92/69, method C.2. Daphnids were exposed to control and test chemical at measured concentration of 0.05, 0.07, 0.20, 0.50, and 0.94 mg/L (geom. mean) for 48 hours. The actual concentrations of the test substance in the test media, determined after 48 hours of exposure, were between 18 and 81% of the actual starting concentrations. The effective concentrations were calculated based on actual concentrations (geom. mean). Mortality and immobilisation were observed daily. The EC50 after 48 hours was 0.11 mg/L (95 % CI 0.10–0.13 mg/L).
The initial concentration and the maintenance of the exposure concentrations during the test were verified in the analytical part. This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results synopsis
Test organism age: < 24 h
Test type: Static
EC50 = 0.11 mg a.i./L (95 % C.I.: 0.10–0.13 mg/L)
EC100 = 0.20 mg/L
EC0 = 0.05 mg/L
Endpoint(s) effected: mobility, mortality
Reference
- ANALYTICAL RESULTS: After 48 hours of exposure, the actual test substance concentrations were between 18 and 81 % of the actual starting concentrations (concentration-dependent). The effective concentrations were calculated based on geometric means of the actual concentrations at the start and end of the test.
- BIOLOGICAL RESULTS: After 48 h exposure 20 % daphnids were immobilised at an exposure concentration of 0.07 mg/L. At the higher concentrations 100 % of the daphnids were immobilised.
VALIDITY CRITERIA:
- Immobilisation of the test organisms in the control at the end of the test was 0 % and therefore ≤ 10 %.
- Dissolved oxygen concentration in the control and the test item treatments at the end of the test was ≥ 8.3 mg/L and therefore ≥ 3 mg/L.
- pH and temperature were sufficiently stable during the test.
Description of key information
In an acute toxicity test according to EEC Commission Directive 92/69, C.2, Daphnia magna were exposed to 0.05, 0.07, 0.20, 0.50, and 0.94 mg ortho-phthalaldehyde/L for 48 hours. The EC50 determined after 48 hours is 0.11 mg/L (95 % CI 0.10–0.13).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.11 mg/L
Additional information
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