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EC number: 203-744-6 | CAS number: 110-18-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 28 June to 30 June 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- In vitro study of skin corrosion, conducted to accepted methodology but prior to development of current guideline.
- GLP compliance:
- no
Test material
- Reference substance name:
- N,N,N',N'-tetramethylethylenediamine
- EC Number:
- 203-744-6
- EC Name:
- N,N,N',N'-tetramethylethylenediamine
- Cas Number:
- 110-18-9
- Molecular formula:
- C6H16N2
- IUPAC Name:
- [2-(dimethylamino)ethyl]dimethylamine
- Test material form:
- liquid
- Details on test material:
- clear, colourless to pale yellow
Constituent 1
- Specific details on test material used for the study:
- Bulab 600
Lot: 3H-9275
Appearance: clear pale yellow liquid
Storage conditions: Room temperature protected from light
In vitro test system
- Test system:
- artificial membrane barrier model
- Source species:
- other: Synthetic
- Cell type:
- other: Hydrated collagen matrix and supporting filter membrane
- Cell source:
- other: Synthetic
- Source strain:
- other: Synthetic
- Justification for test system used:
- The Corrositex assay is a standardised and quantitative in vitro corrosivity test. It is based on the time that is required for the test sample to pass through a biobarrier membrane and produce a change in the Chemical Detection System. The Corrositex Biobarrier Membrane consists of a reconstituted collagen matrix.
- Vehicle:
- unchanged (no vehicle)
- Details on test system:
- The experimental design consists of a pH determination, if possible, and a definitive Corrositex assay in the Chemical Detection System. The Corrositex assay is evaluated on the basis of the colour change of the Chemical Detection System. The time that a colour change is observed is recorded manually and the average of the six replicates is used to determine the Packing Group.
The biobarrier is prepared by adding the biobarrier diluent to the biobarrier matrix powder. This is warmed to 67-69 degrees C to aid solubility. Following this, 200 mcL of the matrix is pipetted onto the membrane discs which are refrigerated at 2-8 degrees C for at least 2 hours, up to 7 days.
Seven vials were used (six for the test material and one for the positive control); 22 mL of the Chemical Detection System was dispensed into each of these vials and 12 mL was dispensed into an eighth vial to serve as the blank control. A membrane disc was placed into one vial which contained 500 mcL of the test material. The vial was observed for three minutes. At one minute intervals, the remaining five membrane discs each containing 500 mcL of test material were added to five further vials. A membrane disc containing a pellet of sodium hydroxide was added to the seventh vial.
The vials were observed continuously for the first 10 minutes, and then at approximately 5 minutes intervals for 4 hours. The first indication of a colour change in the Chemical Detection System (as compared to the blank control) was recorded.
The mean time for the colour to change determined the packing group:
<3 minutes – Packing group I
3 mins to 1 hour – Packing group II
1 to 4 hours – Packing Group III
>4 hours – Non-corrosive - Control samples:
- yes, concurrent positive control
- Amount/concentration applied:
- 500 mcL for the test material.
- Duration of treatment / exposure:
- Up to 4 hours or until a colour change was noted.
- Number of replicates:
- Six
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- penetration time (in minutes)
- Run / experiment:
- 1
- Value:
- >= 40 - <= 43
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks:
- Breakthrough time of 13 min 20 sec
- Remarks on result:
- positive indication of irritation
- Other effects / acceptance of results:
- Positive control response met the acceptance criteria (breakthrough time between 9 min 33 sec and 14 min 01 sec).
Any other information on results incl. tables
Test article |
Breakthrough time (min) |
Packing group |
pH |
||||||
Vial 1 |
Vial 2 |
Vial 3 |
Vial 4 |
Vial 5 |
Vial 6 |
Mean |
|||
Bulab 600 |
43 |
40 |
40 |
41 |
41 |
40 |
40 |
II |
11.0 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (corrosive) based on GHS criteria
- Conclusions:
- The test material was assigned to Packing Group II based on mean breakthrough time of 40 minutes. The results of this study indicate that TMEDA is corrosive to skin
- Executive summary:
Bulab 600 was tested for potential corrosivity using the Corrositex continuous time monitor assay which is a calibrated biobarrier into a chemical detection system. The substance was tested in a single experiment with six replicates. The mean breakthrough time was determined to be 40 minutes and was therefore assigned to Packing Group II. The results of this study indicate that TMEDA is corrosive to skin.
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