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EC number: 915-761-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- None
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adapted 4 April 84
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- EEC Directive 84/449, L 251, Part C 2
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Code number: FAT-31'016/P
- EN-Nr.: 414154.32
- Purity: 95 %
- Appearance: solid
- Solubility: 40 g/L (in water)
- Storage: room temperature
- Expiration date: 07/98 - Analytical monitoring:
- yes
- Details on sampling:
- Sampling for analysis.
All test medium samples for the analytical determinations were taken after mixing of the test solutions from the approximate center of the test vessels and kept at -18 °C to -25 °C until analyses.
The concentrations of the test substance at the beginning and at the end of the test was analysed by the Analytical Services K, Textile Dyes Division. - Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- The test water medium was prepared with bidistilled water.
- Stock solution: 0.5 g test substance were mixed with 200 ml test medium and fill up to 500 ml with test medium. The stock solution was homogenized for 5 minutes in the ultrasonic bath.
- The test medium used had the following composition:
-NaHCO3 65 mg/l
MgSO4 * 7 H2O 123.3 mg/l
KCl 5.8 mg/l
CaCI2 * 2 H2O 294 mg/l
Na2SiO3*9H2O 10 mg/l
NaNO3 274 µg/l
KH2PO4 143 µg/l
K2HPO4 184 µg/l
- The pH was in a range of 7.8 - 8.2.
- The medium was oxygen saturated at the beginning of the test.
- Theoretical water hardness: 136 mg/l CaCO3
- Ca / Mg ratio: 4:1
- Na / K ratio: 10:1
- alkalinity: 0.8 mmol/l - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The Daphnia were bred in the testing facility of the Product Ecology TF, Textile Dyes Division under standardised conditions.
Breeding :
Cultures of Daphnia magna are maintained in glass vessels containing aprox. 3.0 litre of reconstituted water (test medium) at 18 - 22°C. The water was renewed partially three times weekly. The Daphnia were feed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a suspension of TETRAMIN-extract in such quantités that the food was consumed within 24h.\
Pretreatment :
24 hours before the start of the exposure reproductive Daphnia were separated from the young Daphnia by sieving all individuals through a 710 urn sieve.
The quality of the Daphnia is checked at least every 3 months by determining the EC50 values (24 h) for potassium dichromate (range: 0.8 -1.5 mg/l).
For each concentration, of test substance, 20 Daphnia divided in two groups of 10 animals are used. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- Theoretical water hardness: 136 mg/l CaCO3
- Test temperature:
- 21±1 °C
- pH:
- 7.7 - 8.2.
- Dissolved oxygen:
- 92 - 99 %
- Nominal and measured concentrations:
- Based on a pretest, the test concentrations were chosen as 0.58 /1.0 /1.8 / 3.2 / 5.8 and 10.0 mg test article / I nominal.
- Details on test conditions:
- 48 hours at 21 °C, 16 hours light / 8 hours dark
The Daphnia were exposed to the test article in 250 ml beakers containing 100 - 200 ml test medium with 10 animals per beaker. All tests were run in duplicate. During exposure the animals were kept at 21 ± 1°C with 16 hours illumination.
- Determination of results
Test evaluations were done according to the guidelines by visual assessment of mobility of the Daphnia after 24 and 48 hours. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 3.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- In control and test concentrations 0.5 and 1.0 mg/l , no immobilisation was observed. At 1.8 mg/l, 80 % immobility was observed at the end of 48 hour observation period. At test concentrations 3.3, 5.8 and 10.0 mg/l, 100 % immobility was observed.
- Results with reference substance (positive control):
- Potassium dichromate (EC50: 1.01 mg/l)
- Reported statistics and error estimates:
- The EC50 - value are calculated with the " Spearman - Kaerber's " method.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 value was determined to be 1.5 mg/l.
- Executive summary:
The acute toxicity of FAT - 31'016/P to Daphnia magna was determined in a 48-hours static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adapted 4
April 84, and EEC Directive 84/449, L 251, Part C 2. The test was performed in compliance with the Good Laboratory Practice Regulations. The nominal concentrations tested were 0.58 /1.0 /1.8 / 3.2 / 5.8 and 10.0 mg test article / l, and a control without any additions. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 80.0 - 120.0 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable. In all test concentrations non alteration of the test substance after 48 hours was observed. In the control non immobilization of the Daphnia magna was observed after 48 hours. At test concentrations 0.5 and 1.0 mg/l , no immobilisation was observed, while at 1.8 mg/l, 80 % immobility was observed at the end of 48 hour observation period. At test concentrations 3.3, 5.8 and 10.0 mg/l, 100 % immobility was observed. Hence, the EC0 value was at least 1.0 mg/l, but might even be higher than this concentration. The EC50 value was determined to be 1.5 mg/l, while the EC100 value was 3.3 mg/l after 48 hours.
Reference
The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 80.0 - 120.0 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable.
Description of key information
The EC50 value to Daphnia magna was determined to be 1.5 mg/l, while the EC100 value was 3.3 mg/l after 48 hours.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.5 mg/L
Additional information
Basic Red 046 Methylsulfate has not been investigated for short term toxicity to Daphnia. However, acute toxicity of structurally similar substance Basic Red 046 Bromide to Daphnia magna was determined in a 48-hours static test conducted according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adapted 4 April 84, and EEC Directive 84/449, L 251, Part C 2. The nominal concentrations tested were 0.58 /1.0 /1.8 / 3.2 / 5.8 and 10.0 mg test article / l, and a control without any additions. The reported results are related to the analytical concentrations of the test article. During test duration the test article concentrations in the analysed test media were in the range of 80.0 - 120.0 % of the nominal values. Therefore, under test conditions the test article was sufficiently stable. In all test concentrations non alteration of the test substance after 48 hours was observed. In the control non immobilization of the Daphnia magna was observed after 48 hours. At test concentrations 0.5 and 1.0 mg/l , no immobilisation was observed, while at 1.8 mg/l, 80% immobility was observed at the end of 48 hour observation period. At test concentrations 3.3, 5.8 and 10.0 mg/l, 100 % immobility was observed. Hence, the EC0 value was at least 1.0 mg/l, but might even be higher than this concentration. The EC50 value was determined to be 1.5 mg/l, while the EC100 value was 3.3 mg/l after 48 hours.
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