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Diss Factsheets
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EC number: 295-184-4 | CAS number: 91845-13-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The study was performed between 07 April 2009 and 09 April 2009.
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Non-GLP study with no analytical monitoring.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- see "Principles of method if other than guideline"
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- yes
- Remarks:
- see "Principles of method if other than guideline"
- Principles of method if other than guideline:
- The temperature was 1-2 °C above the range required by the guidelines. As no abnormalities were observed in the controls, it was considered as non-critical.
- GLP compliance:
- no
- Remarks:
- The study was commissioned for non-REACH purposes for which GLP compliance was not a requirement.
Test material
- Reference substance name:
- Additiv 309
- IUPAC Name:
- Additiv 309
- Details on test material:
- Test number: 09032301 H
pH of the soln diluted in water: 7.5 (100 mg/L)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was stored in a sealed container at room temperature. Due to the low water solubility, the water-accommodated fractions (WAF) of the required concentrations were used. The WAF is produced by weighing of each nominal load, 24-hour shaking with the required amount of dilution water (synthetic dilution water according to ISO 6341) for testing and subsequent membrane filtration.
- Differential loading: 0, 1, 10 and 100 mg/L were tested
- Controls: potassium dichromate
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: Berlin
- Age of parental stock (mean and range, SD): For the test young animals at the age of 0-24 hours were used.
- Feeding during test
- Food type: green algae (Desmodesmus subspicatus)
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg CaCO3/L
- Test temperature:
- Temperature: 20 ± 2 ° C (maximum deviation within one test ± 1 °C)
- pH:
- 7.8 ± 0.2
- Nominal and measured concentrations:
- 0, 1, 10 and 100 mg/L (WAF)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50-mL beakers according to DIN
- No. of organisms per vessel: Use of each 5 animals in 20 mL of sample
- No. of vessels per concentration (replicates): Pre-tests were performed in duplicate, major test were conducted four times.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic dilution water prepared according to ISO 6341
OTHER TEST CONDITIONS
- Adjustment of pH: After preparation, the dilution water aerated and pH adjusted to 7.8 ± 0.2
- Photoperiod: 16/8-h light–dark rhythm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : counting the immobilised animals after 24 and 48hours - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: water accommodated fraction
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: water accommodated fraction
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: water accommodated fraction
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: water accommodated fraction
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: water accommodated fraction
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: water accommodated fraction
- Details on results:
- As NOEC an immobilisation of max. 10% was considered (criterion for maximum immobilisation in the control).
- Results with reference substance (positive control):
- In a current reference test the 24hEC50i was 1.8 mg/L. Validity: the 24hEC50i of the reference substance potassium dichromate is supposed to be between 0.6 to 2.1 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 48h EC50i > 10 mg/L
NOEC 48h = 10 mg/L - Executive summary:
The test was conducted in accordance with the OECD Guideline 202 and EU Method C.2. The swimming capability or immobilisation of Daphnia Magna was used as the endpoint in determining the acute toxicity of the test item. Daphnids (< 24 hours old) were exposed to the WAF’s (0mg/L, 1 mg/L, 10mg/L and 100mg/L) over a period of 48 hours. Exposures were performed in quadruplicate in the main test (duplicates in pre-tests) using five animals in each replicate (20 mL of sample). Immobilised daphnids were recorded at 24 and 48 hours of the test. The biological results can be summarised as follows:
– 24-hour NOEC = 10 mg/L
– 24-hour EC50i = 100 mg/L
– 24-hour EC100i > 100 mg/L
48-hour NOEC = 10 mg/L
– 48-hour EC50i > 10 mg/L
– 48-hour EC100i 100 mg/L
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