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EC number: 290-971-9 | CAS number: 90294-83-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2017-07-20 to 2017-08-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
- Reference Type:
- other: amendment to the study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Cobalt, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, sulfonated, sodium salts
- EC Number:
- 290-971-9
- EC Name:
- Cobalt, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, sulfonated, sodium salts
- Cas Number:
- 90294-83-0
- Molecular formula:
- CoC32H16-τN8(SO3Na)τ
- IUPAC Name:
- Cobalt, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, sulfonated, sodium salts
- Test material form:
- solid: particulate/powder
1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal sewage treatment plant, 31137 Hildesheim, Germany.
Activated sludge from the sewage plant at Hildesheim is well suited as it receives predominantly municipal sewage and hardly any industrial chemical waste. (Receipt : 2017-07-13)
- Preparation of inoculum for exposure: Colony forming units (CFU) of the inoculum for the Modified Sturm Test were determined prior to test start by standard dilution plate count: approx. 4.1 x 10^9 CFU/L, corresponding to approx. 4.1 x 10^7 CFU/L in the test vessel. The concentration of the test item and the theoretical CO2 production (ThCO2) were calculated based on the carbon content.
The necessary amounts of ultrapure water, mineral salts medium and inoculum were placed in each incubation vessel. The vessels were aerated for 24 h with CO2 free air.
- Initial cell/biomass concentration: Approx. 10^7 - 10^8 CFU/L - Duration of test (contact time):
- ca. 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- ca. 29 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 10.4 other: mg C/L
- Based on:
- TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral salts medium according to OECD 301B/CO2 evoluation test.
- Test temperature: measured range between 20.5 and 24°C
- Aeration of dilution water: 30 - 100 mL/min
- Continuous darkness: Low light conditions (brown glass bottles)
TEST SYSTEM
- Culturing apparatus: brown glass vessel (capacity 5000mL), where 3000mL of test medium is added. Continous stirring of the medium during the experiment.
- Number of culture flasks/concentration:
* Functional control (Sodium benzoate) : 1 replicate
* Toxicity control: 1 replicate
* Inoculum control : 2 replicates
* Test item : 2 replicates
- Method used to create aerobic conditions: The vessels were aerated for 24 h with CO2 free air. After 24 h the CO2 adsorption vessels were connected to the air outlets of the incubation vessels via a series of 3 gas wash bottles, each containing 100 mL of a 0.0125 mol/L Ba(OH)2 solution.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Test medium without test and/or reference item
- Toxicity control: Test item and reference item in test concentration
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- The study was performed according to OECD 301 B and GLP Guidelines. The validity criteria were fulfilled according to the guideline:
•The total CO2 evolution in the inoculum control at the end of the test was 20.4 mg/L
(validity criterion: < 40 mg CO2 /L after 28 days).
•The degradation of the functional control reached the pass level of >= 60% within 8 days (degradation: 70% on day 8).
•The differences of extremes of replicate values of removal of the test item at the end of the test was less than 20% (11% difference on day 28).
•The degradation of the toxicity control reached the pass level of 25% within 6 days (degradation: 32% on day 6).
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 21
- Sampling time:
- 28 d
- Details on results:
- The mean of replicates reached the 10% level (beginning of biodegradation) on day 11. Both test item replicates did not reach the 60% pass level within the 28-day period of the study. The mean biodegradation on day 28 was 21%.
Any other information on results incl. tables
Table 1: CO2-Production and Biodegradation after 28 Days
CO2 Production | Functional Control | Test Item | Toxicity Control test item + reference item | |
1 | 2 | |||
Net[mg/3 L] | 121.2 | 17.4 | 29.6 | 122.9 |
[mg/L] | 40.4 | 5.8 | 9.9 | 41.0 |
Theor.[mg/3 L] | 127.8 | 114.8 | 242.6 | |
[mg/L] | 42.6 | 38.3 | 80.9 | |
Degradation [%] | 95 | 15 | 26 | 51 |
Table 2: CO2-Production and
Biodegradation in the Inoculum Control, the Functional
Controland the Toxicity Control
Study Day | Date | Inoculum control | Functional Control | Toxicity Control | ||||
[mg CO2/3 L] | [mg CO2/3 L] | Degr. | [mg CO2/3 L] | Degr. | ||||
mv | gross | net | % | gross | net | % | ||
1 | 21/07/2017 | 3.0 | 13.8 | 10.8 | 8 | 3.9 | 0.9 | 0 |
4 | 24/07/2017 | 6.3 | 40.2 | 44.7 | 35 | 51.9 | 46.5 | 19 |
6 | 26/07/2017 | 7.0 | 35.9 | 73.6 | 58 | 37.1 | 76.6 | 32 |
8 | 28/07/2017 | 7.0 | 23.2 | 89.8 | 70 | 23.9 | 93.5 | 39 |
11 | 31/07/2017 | 5.1 | 17.6 | 102.3 | 80 | 10.9 | 99.3 | 41 |
14 | 03/08/2017 | 5.5 | 14.3 | 111.1 | 87 | 12.3 | 106.1 | 44 |
18 | 07/08/2017 | 5.3 | 11.0 | 116.8 | 91 | 11.2 | 112.0 | 46 |
22 | 11/08/2017 | 6.9 | 9.8 | 119.7 | 94 | 11.8 | 116.9 | 48 |
25 | 14/08/2017 | 5.5 | 5.9 | 120.1 | 94 | 9.9 | 121.3 | 50 |
28 | 17/08/2017 | 4.4 | 5.3 | 121.0 | 95 | 5.5 | 122.4 | 50 |
29* | 18/08/2017 | 5.1 | 5.3 | 121.2 | 95 | 5.6 | 122.9 | 51 |
Degr. = degradation mv = mean value *) results of last two gas wash bottles
In the inoculum controlthe total CO2production was 20.4 mg CO2/L after 28 days.
Table 3: O2-Production and Biodegradation in the Inoculum Control and Test Item Samples
Study Day | Date | Inoculum Control | Test Item | ||||||
Replicate 1 | Replicate 2 | ||||||||
[mg CO2/3 L] | [mg CO2/3 L] | Degr. | [mg CO2/3 L] | Degr. | |||||
mv | Gross | Net Sum | [%] | Gross | Net Sum | [%] | |||
1 | 21/07/2017 | 3.0 | 3.6 | 0.6 | 1 | 4.3 | 1.3 | 1 | |
4 | 24/07/2017 | 6.3 | 11.0 | 5.3 | 5 | 13.5 | 8.5 | 7 | |
6 | 26/07/2017 | 7.0 | 8.6 | 6.9 | 6 | 10.5 | 12.0 | 10 | |
8 | 28/07/2017 | 7.0 | 9.8 | 9.7 | 8 | 7.9 | 12.9 | 11 | |
11 | 31/07/2017 | 5.1 | 5.8 | 10.4 | 9 | 6.1 | 13.9 | 12 | |
14 | 03/08/2017 | 5.5 | 6.5 | 11.4 | 10 | 7.9 | 16.3 | 14 | |
18 | 07/08/2017 | 5.3 | 8.4 | 14.5 | 13 | 9.9 | 20.9 | 18 | |
22 | 11/08/2017 | 6.9 | 9.4 | 17.0 | 15 | 11.7 | 25.7 | 22 | |
25 | 14/08/2017 | 5.5 | 5.1 | 17.0 | 15 | 6.9 | 27.1 | 24 | |
28 | 17/08/2017 | 4.4 | 4.8 | 17.4 | 15 | 5.8 | 28.5 | 25 | |
29* | 18/08/2017 | 5.1 | 3.4 | 17.4 | 15 | 6.2 | 29.6 | 26 |
Table 4 : pH-Values on Day 28
Inoculum Control |
Func-tional |
Test Item |
Toxicity Control |
||
No. 1 |
No. 2 |
No.1 |
No. 1 |
No. 2 |
No. 1 |
7.72 |
7.69 |
7.77 |
7.65 |
7.67 |
7.76 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Under the test conditions the test item is classified as not readily biodegradable within the 28 day period of the study.
- Executive summary:
The ready biodegradability of the test item was determined with a non-adapted activated sludge over a test period of 28 days in the Modified Sturm Test. The study was conducted from 2017-07-20 to 2017-08-18, according to OECD 301 B at the test facility.
The test item was tested at a concentration of 29 mg/L with 2 replicates corresponding to a carbon content (TOC) of 10.4 mg C/L in the test vessels.The test vessels were incubated at low light conditions and at a temperature of 22 ± 2 °C.
The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO2 produced by the respiration of bacteria. The degradation was stopped on day 28 by acidification of the test solutions. The last titration was made on day 29 after residual CO2 had been purged from the test solutions over a period of 24 hours. The percentage CO2 production was calculated in relation to the theoretical CO2 production (ThCO2) of the test item. The biodegradation was calculated for each titration time.
To check the activity of the test system sodium benzoate was used as functional control. The percentage degradation of the functional control reached the pass level of 60% within 8 days and a maximum biodegradation of 95% on day 28.
In the toxicity control containing both test and reference item a biodegradation of 44% was determined within 14 days and it came to 51% after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control.
The biodegradation of the test item is shown graphically in Figure 1 in comparison to the readily degradable functional control and the toxicity control. The mean of replicates reached the 10% level (beginning of biodegradation) on day 11. Both test item replicates did not reach the 60% pass level within the 28-day-period of the study. The mean biodegradation on day 28 was 21%.
Under the test conditions the test item is classified as not readily biodegradable within the 28 day period of the study.
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