Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 246-002-7 | CAS number: 24057-28-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Version / remarks:
- May 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Version / remarks:
- July 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Version / remarks:
- 1998
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): local wastewater treatment plant, ARA Birs (Birsfelden / Switzerland)
- Method of cultivation / Preparation of inoculum for exposure: The aerobic activated sewage sludge was washed three times by centrifugation, decantation of the supernatant liquid phase and resuspension of the solid material in tap water and finally in mineral medium. Aliquots of the homogenized final sludge suspension were weighed, thereafter dried and the dry weight of the suspended solids was determined.
Based on this determination, a calculated amount of wet sludge was suspended in mineral medium to obtain a concentration equivalent to 4 g dry material per liter. During the holding period of three days prior to use, the sludge was aerated at room temperature. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 30 mg/L
- Based on:
- DOC
- Initial conc.:
- 54 mg/L
- Based on:
- test mat.
- Remarks:
- corrected for the water content
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to testing guidelines; analytical grade salts were dissolved in ultrapure water (ELGA PURELAB Option-Q water purification system)
- Test temperature: 22 °C
- pH: 7.4 at the start of the test and 7.0 to 7.4 at the end
- pH adjusted: no
- Suspended solids concentration: 30 mg/L
- Continuous darkness: no (diffuse light)
TEST SYSTEM
- Culturing apparatus: 2000 mL Erlenmeyer glass vessels, filled to a volume of 1000 mL, loosely covered with an aluminum cap to reduce losses by evaporation. The test vessels were incubated in an incubator with automatic temperature control and equipped with an orbital platform shaker, at approximately 130 rpm to prevent settlement of the inoculum and to maintain aerobic conditions.
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: orbital shaking at approximately 130 rpm
- Measuring equipment: TOC infrared gas analyzer equipped with an automatic sampler
- Test performed in open system: yes
SAMPLING
- Sampling frequency: Test item and inoculum control: Exposure Day 0, 2, 5, 7 and 8; procedure and toxicity control: Exposure Day 0, 2, 7 and 8. Sampling was ended before 28 days because the biodegradation curve reached a plateau for three determinations.
- Sampling method: Prior to sampling, water evaporation losses were determined by weighing the vessels and were compensated by adding purified water. Deposits on the test vessels were resuspended. Then, one sample of about 10 mL was taken from each sampled test vessel per sampling occasion. Samples were filtered through a 0.45 µm filter. The first 2-3 mL of the filtrate were discarded.
- Sample storage before analysis: Samples were immediately analyzed for DOC.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks containing inoculated mineral medium
- Reference: 2 flasks containing inoculated mineral medium and sodium benzoate (31 mg DOC/L)
- Toxicity control: 1 flask containing inoculated mineral medium, test material (31 mg DOC/L) and sodium benzoate (31 mg DOC/L) - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 97
- Sampling time:
- 8 d
- Details on results:
- The test was discontinued after 8 days since the test material was completely degraded.
Average biodegradation the toxicity control was >35 % by Exposure Day 8, indicating that the test substance was not inhibitory to activated sludge at the tested concentration of 54 mg/L.
For the individual results see attached document "Tables - DOC concentrations and % biodegradation.pdf" - Results with reference substance:
- Average biodegradation of the reference item sodium benzoate was >60 % by Exposure Day 8, thus confirming suitability of the activated sludge, thus confirming the suitability of the used activated sludge inoculum (see "Attached background material" – "Tables - DOC concentrations and % biodegradation.pdf").
- Validity criteria fulfilled:
- yes
- Remarks:
- The difference between the degradation values of the replicate test vessels containing the test item was less than 20 % at the end of the test; the percentage degradation of the reference item reached > 70 % in a 10-day window by Day 14.
- Interpretation of results:
- readily biodegradable
Reference
Description of key information
Biodegradation in water: screening tests: aerobic biodegradation >90% (DOC) in 28 days (OECD 301A, EU C.4 -A)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
[Type of water: freshwater]
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.