Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
Reaction mass of sulfonium, dodecylethyl[1-(2-methoxy-2-oxoethyl)-3-oxo-3-(pentyloxy)propyl]-, tetrafluoroborate(1-)(1:1) and sulfonium, dodecylethyl[3-methoxy-1-(2-methoxy-2-oxoethyl)-3-oxopropyl]-, tetrafluoroborate(1-)(1:1) and sulfonium, dodecylethyl[3-oxo-1-[2-oxo-2-(pentyloxy)ethyl]-3-(pentyloxy)propyl]-, tetrafluoroborate(1-)(1:1)
EC number: 943-993-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- (1,5-dimethoxy-1,5-dioxopentan-3-yl)(dodecyl)ethylsulfanium; [1,5-dioxo-1,5-bis(pentyloxy)pentan-3-yl](dodecyl)ethylsulfanium; dodecyl(ethyl)[1-methoxy-1,5-dioxo-5-(pentyloxy)pentan-3-yl]sulfanium; tris(tetrafluoroboranuide)
- EC Number:
- 943-993-4
- Cas Number:
- 2220260-54-6
- Molecular formula:
- not applicable for multi-constituent.
- IUPAC Name:
- (1,5-dimethoxy-1,5-dioxopentan-3-yl)(dodecyl)ethylsulfanium; [1,5-dioxo-1,5-bis(pentyloxy)pentan-3-yl](dodecyl)ethylsulfanium; dodecyl(ethyl)[1-methoxy-1,5-dioxo-5-(pentyloxy)pentan-3-yl]sulfanium; tris(tetrafluoroboranuide)
- Test material form:
- liquid
- Details on test material:
- Multi-constituent substance.
degree of purity: >=94%
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Batch 624730
- Expiration date of the lot/batch: 2017-12
- Purity test date: 05 May, 2017
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: No data
- Solubility and stability of the test substance in the solvent/vehicle: Dosed neat
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dosed neat
In vitro test system
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- non-transformed keratinocytes
- Cell source:
- other: No data
- Source strain:
- other: Not applicable
- Vehicle:
- unchanged (no vehicle)
- Details on test system:
- RECONSTRUCTED HUMAN EPIDERMIS (RHE) TISSUE
- Model used: MatTek EpiDerm
- Tissue batch number(s): Lot No. 26717 Kit D and Lot No. 24973 Kit D (Frozen cells)
- Production date:
- Shipping date: No data
- Delivery date: 25 July, 2017
- Date of initiation of testing: 25 July, 2017
TEMPERATURE USED FOR TEST SYSTEM
- Temperature used during treatment / exposure: 37 C
- Temperature of post-treatment incubation (if applicable): 37 C
REMOVAL OF TEST MATERIAL AND CONTROLS
-Volume and number of washing steps: Each tissue was rinsed with phosphate buffered saline (volume and number of steps were not specified).
- Observable damage in the tissue due to washing: No data
MTT DYE USED TO MEASURE TISSUE VIABILITY AFTER TREATMENT / EXPOSURE
- MTT concentration: 1 mg/mL MTT in DMEM
- Incubation time: 3 hours
- Spectrophotometer: uQuant Plate Reader, Bio-Tek Instruments, Winooski, VT
- Wavelength: 540 nm
NUMBER OF REPLICATE TISSUES: 2 per treatment
CONTROL TISSUES USED IN CASE OF MTT DIRECT INTERFERENCE
- Fresh tissues / killed tissues: Frozen tissues were run concurently at the 60 minute timepoint for the test article as it reduced MTT. Control frozen tissues were run in parallel.
- Procedure used to prepare the killed tissues (if applicable): No data
- N. of replicates : 2
- Method of calculation used: Corrected Viability = VA – VB - VC
Where:
VA = Mean Viability of live tissues treated with the test article
VB = Mean Apparent Viability of frozen (dead) tissues treated with the test article
VC = Mean Apparent Viability of frozen (dead) tissues treated with TCH2O
NUMBER OF INDEPENDENT TEST SEQUENCES / EXPERIMENTS TO DERIVE FINAL PREDICTION: The average of the values from the duplicate runs were used.
PREDICTION MODEL / DECISION CRITERIA (choose relevant statement)
- The test substance is considered to be corrosive to skin if the viability after 3 minutes exposure is less than 50%, or if the viability after 3 minutes exposure is greater than or equal to 50 % and the viability after 1 hour exposure is less than 15%.
- The test substance is considered to be non-corrosive to skin if the viability after 3 minutes exposure is greater than or equal to 50% and the viability after 1 hour exposure is greater than or equal to 15%. - Control samples:
- yes, concurrent negative control
- yes, concurrent positive control
- Amount/concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 uL
NEGATIVE CONTROL
- Amount(s) applied (volume or weight): 50 uL
POSITIVE CONTROL
- Amount(s) applied (volume or weight): 50 uL
- Concentration (if solution): 8.0 N - Duration of treatment / exposure:
- 3, 60 minutes
- Duration of post-treatment incubation (if applicable):
- 3 hour MTT incubation, overnight extractant incubation.
- Number of replicates:
- 2
Results and discussion
In vitro
Resultsopen allclose all
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 3 minute exposure
- Value:
- 88.9
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 60 minutes exposure
- Value:
- 77.6
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritant) based on GHS criteria
- Conclusions:
- Based on the results of the study, MTDID 47403 is non-corrosive.
- Executive summary:
The in vitro skin irritation/corrosion potential of the test article was evaluated using the MatTek EpiDerm Skin Corrosivity Test. The study was performed in compliance with GLP. The study design was based on the MatTek MTT Effective Time-50 (ET-50) Protocol (protocol no. 711-02) and OECD 431. The test article was dosed neat. Since the test article reduced MTT, frozen (dead) tissues were tested concurrently at 60 minutes and a corrected tissue viablility was calculated. EpiDerm samples were incubated in 6-well tissue culture plates containing Dulbecco's Modified Eagle's Medium (DMEM) for 1 hour. After incubation, 50 uL of the test article was applied to the tissues on top of a mesh spreading aid. The test article remained in contact with the tissue for 3 and 60 minutes. A negative control (50 uL of TCH2O) and a positive control (50 uL of potassium hydroxide solution, 8.0 N) were tested in parallel. Each treatment with test article or control was conducted in duplicate. At the end of the exposure period, each EpiDerm tissue was rinsed with phosphate buffer saline (PBS) and transferred to a 24-well microplate containing 300 uL of MTT solution (1 mg/mL MTT in Dulbecco's Modified Eagle's Medium (DMEM); a negative control, 100 uL of TCH2O was tested concurrently. Tissues were then returned to the incubator for a 3-hour MTT incubation period. Following the MTT incubation period, each EpiDerm™ tissue was rinsed with PBS and then treated overnight with 2.0 ml of extractant solution (isopropanol) per well. The absorbency of an aliquot of the extracted MTT formazan was measured in triplicate at 540 nm using a microplate reader. The mean absorbance value for each time point was calculated from the optical density (OD) of the duplicate tissues and expressed as percent viability for each sample. A mean viability at 3 minutes of greater than or equal to 50% and a mean viability at 60 minutes greater than or equal to 15% indicates the material was non-corrosive. The positive and negative controls performed as expected which indicated test validity. The corrected cell viability for tissue treated with the test material was 88.9% and 77.6% at 3 and 60 minutes, respectively. A skin irritation potential cannot be excluded by this test and therefore the substance was classified as a Category 2 skin irritant according to the GHS criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.