Fatty acids, coco, iso-Bu esters

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

22 - 26 May 1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)

Deviations:

yes

Remarks:

No control was conducted.

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Isopropyl palmitate

EC Number:

205-571-1

EC Name:

Isopropyl palmitate

Cas Number:

142-91-6

Molecular formula:

C19H38O2

IUPAC Name:

isopropyl palmitate

Details on test material:

- Name of test material (as cited in study report): Hexadecanoic acid, 1-methylethyl ester
- Analytical purity: 100% AM

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct addition of TS filled into vessels with 5 L standard dilution water, stirring 5 minutes by ultraturrax

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Feeding during test: none

ACCLIMATION
- Health during acclimation (any mortality observed): <0.1%

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

approx. 250 mg CaCO3/L

Test temperature:

22.5 - 23.5 °C

pH:

8.2 - 8.3

Dissolved oxygen:

66 - 97%

Nominal and measured concentrations:

nominal: 1000, 3000 and 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L glass aquaria
- Material, size, headspace, fill volume: 5 L fill volume
- Renewal rate of test solution (frequency/flow rate): every 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: standard dilution water

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/ 8 hours darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, recorded after 6, 24, 48, 72 and 96 hours

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none observed

Any other information on results incl. tables

Sublethal observations / clinical signs:

The test has been conducted in 1982 following a company internal test guideline. The test is set up as a screening test for the evaluation of the non hazardous concentration of chemicals for aquatic organisms. The test principle is to expose fish (Brachydanio rerio) to increasing concentrations of a test substance and determine the highest concentration at which no mortality is observed. 1.000 mg/L was chosen as the highest test substance concentration, and additionally the concentrations 300 mg/L and 100 mg/L were tested. As the results clearly show, no mortality occurred at any of the tested concentrations. Therefore, it can be concluded that isopropyl palmitate in not acutely toxic to fish at concentrations ≤ 1000 mg/L. Although no blank (without test substance) has been included in the test, the test with 100 mg/L can be regarded as internal control that the animals in the test were healthy. Taking into account that this test is not regarded as a standard OECD 202, but as a toxicity limit screening test, a Klimisch grade 2 is justifiable.

Table 1. Mortality of zebrafish in %:

	Concentration	Incubation time (h)
in mg/L		0	6	24	48	72	96
100		0	0	0	0	0	0
300		0	0	0	0	0	0
1000		0	0	0	0	0	0