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EC number: 263-179-6 | CAS number: 61791-46-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019-03-04 to 2019-04-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- A water accommodated fraction (WAF) equilibration trail was performed prior to exposure. The purpose of the equilibration trail is to determine the optimum mixing duration to be used for WAF preparation.
One WAF was prepared at each of three loading levels 1.0, 50.0 and 100 mg/L. At 2, 4, 6 and 24 hours after initiation of mixing, mixing was stopped and the solutions were allowed to settle for one hour. A sample of WAF was removed from each loading level and mixing was resumed.
The concentration of organic carbon that had solubilized into the WAF from the test substance was measured using the TOC analyzer (TOC Analyzer/TNML; Make: Shimadzu; Model: LCPH E200 ROHS). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Waterflea
- Strain: Straus
- Source: Environmental and Molecular Toxicology Division, Department of Zoology, Karnataka University, Karnataka, India
- Feeding during test: no
- Age of test animals at start of treatment: Less than 24 h old
ACCLIMATION
- Acclimation period: 48h
- Acclimation conditions: Same as test conditions
- Type and amount of food: Live yeast (Saccharomyces subcapitata) or algal cells (Pseudokirchneriella subcapitata) of 2 mL per liter
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not performed
- Hardness:
- Dose Range Finding study and Main study: 224 mg/L CaCO3
- Test temperature:
- Dose Range Finding Study: 19.9 - 21.5 °C
Main study: 20.0 - 21.1 °C - pH:
- Dose Range Finding Study: 7.11 - 7.45
Main study: 7.13 - 7.53 - Dissolved oxygen:
- Dose Range Finding Study: 7.06 - 8.18 mg/L
Main study: 7.19 - 7.96 mg/L - Nominal and measured concentrations:
- Nominal loading rates: 0.010, 0.021, 0.047, 0.103, 0.227 and 0.500 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Rectangular vessels
- Type: open
- Material, size: Glass, holding capacity of 100 mL
- Aeration during test: No
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: Reconstituted water with the pH 6.0 to 9.0, total hardness between 140 to 250 mg/L (as CaCO3). The dilution water was areated prior to use for the test so that the dissolved oxygen concentration has reached saturation.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light / 8 hours darkness
EFFECT PARAMETERS MEASURED: Mobility
RANGE-FINDING STUDY
- Test concentrations: 6 concentrations of 0.01, 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L along with a negative control
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 0.082 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI: 0.0696 - 0.0954
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 0.021 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- LOELR
- Effect conc.:
- 0.047 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- No clinical signs of toxicity or immobilization were observed in control and at the nominal loading rate of 0.010 mg/L during the 48 hours observation period.
Clinical signs of lethargy and daphnids localized on bottom of container was observed at 24 and 48 hours observation at the nominal loading rates of 0.021, 0.047, 0.103 and 0.227 mg/L.
Clinical signs of lethargy and daphnids localized on bottom of container was observed at 24 hours observation at the nominal loading rate of 0.500 mg/L.
The immobilization percentages of 0, 10, 35, 60, 80 and 100 % were observed at the nominal loading rates of 0.010, 0.021, 0.047, 0.103, 0.227 and 0.500 mg/L, respectively during the 48 hours observation period. - Results with reference substance (positive control):
- - Results with reference substance valid?
Yes
- 48h-EC50: 0.58 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- In the acute immobilisation test according to OECD guideline 202 the 48 hours acute median Effective Loading Rate (EL50) of Humectol LYS MOD on Daphnia magna is 0.0815 mg/L, the 95% confidence interval with lower limit of 0.0696 mg/L and upper limit of 0.0954 mg/L. No Observed Effect Loading Rate (NOELR) and Lowest Observed Effect Loading Rate (LOELR) over the 48 hours exposure period are 0.021 and 0.047 mg/L of Humectol LYS MOD respectively.
- Executive summary:
The test item Humectol LYS MOD was evaluated for acute toxicity on Daphnia magna according to OECD Guideline No. 202. The acute toxicity study of Humectol LYS MOD on Daphnia magna was performed to determine the 48 hours Effective concentration (EL50) and to determine Lowest Observed Effect Nominal Loading Rate (LOELR) and No Observed Effect Nominal Loading Rate (NOELR). Daphnids were exposed for the test item by aqueous exposure (treatment) under semi-static condition. Daphnids were observed at 24 and 48 hours for signs of toxicity and immobilization.
Based on the results of a dose range finding study, main study was conducted at the nominal loading rates of 0.010, 0.021, 0.047, 0.103, 0.227 and 0.500 mg/L of Humectol LYS MOD of test medium along with control. No clinical signs of toxicity were observed in control and at the nominal loading rate of 0.010 mg/L during the 48 hours observation period. Clinical signs of daphnids localized on bottom of container and lethargy was observed at the nominal loading rates of 0.021, 0.047, 0.103 and 0.227 mg/L during the 48 hours observation period. Clinical signs of daphnids localized on bottom of container and lethargy was observed at the nominal loading rates of 0.500 mg/L during the 24 hours observation period. The immobilization percentages of 0, 10, 35, 60, 80 and 100% were observed at the nominal loading rates of 0.010, 0.021, 0.047, 0.103, 0.227 and 0.500 mg/L respectively during the 48 hours observation period.
Based on the above results of the experiment and under experimental conditions employed, it is concluded that the 48 hour acute median effective concentration (EL50) of Humectol LYS MOD on Daphnia magna is 0.0815 mg/L; the 95 % confidence interval with lower limit of 0.0696 mg/L and upper limit of 0.0954 mg/L. NOELR and LOELR over the 48 hours exposure period are 0.021 and 0.047 mg/L of Humectol LYS MOD, respectively.
Reference
Validity criteria of the test
- Immobilisation in the control
group was 0 % at the termination of the test during main study (validity
criterion: should not exceed 10 %).
- Dissolved oxygen concentration at the end of the test during main study was 7.96 mg/L (Validity criterion: should be ≥ 3 mg/L).
Table 1: Summary of clinical signs and immobilization during main study
Group |
Nominal loading rate |
R |
No. of Daphnids per replicate at start 0 h |
Signs of toxicity and Immobilisation of Daphnids Observed at |
|||
24 h |
48 h |
||||||
Toxic signs |
I |
Toxic signs |
I |
||||
G1 |
0.0 |
R1 |
5 |
N(5) |
0 |
N(5) |
0 |
R2 |
5 |
N(5) |
0 |
N(5) |
0 |
||
R3 |
5 |
N(5) |
0 |
N(5) |
0 |
||
R4 |
5 |
N(5) |
0 |
N(5) |
0 |
||
G2 |
0.010 |
R1 |
5 |
N(5) |
0 |
N(5) |
0 |
R2 |
5 |
N(5) |
0 |
N(5) |
0 |
||
R3 |
5 |
N(5) |
0 |
N(5) |
0 |
||
R4 |
5 |
N(5) |
0 |
N(5) |
0 |
||
G3 |
0.021 |
R1 |
5 |
N(4), B(1) |
0 |
N(3), B(1) |
1 |
R2 |
5 |
N(4), L(1) |
0 |
N(3), B(1) |
1 |
||
R3 |
5 |
N(3), L(2) |
0 |
N(3), L(2) |
0 |
||
R4 |
5 |
N(3), L(1), B(1) |
0 |
N(2), L(1), B(2) |
0 |
||
G4 |
0.047 |
R1 |
5 |
N(2), L(1), B(1) |
1 |
N(2), L(1) |
1 |
R2 |
5 |
N(2), B(1) |
2 |
N(1), L(1), B(1) |
0 |
||
R3 |
5 |
N(2), B(3) |
0 |
N(2), B(2) |
1 |
||
R4 |
5 |
N(3), L(1) |
1 |
N(2), L(1) |
1 |
||
G5 |
0.103 |
R1 |
5 |
N(1), B(2) |
2 |
B(2) |
1 |
R2 |
5 |
N(1), L(1), B(2) |
1 |
L(1), B(1) |
2 |
||
R3 |
5 |
N(1), L(2), B(1) |
1 |
L(1), B(1) |
2 |
||
R4 |
5 |
N(1), L(2) |
2 |
L(2) |
1 |
||
G6 |
0.227 |
R1 |
5 |
L(1), B(1) |
3 |
L(1) |
1 |
R2 |
5 |
L(2), B(1) |
2 |
B(1) |
2 |
||
R3 |
5 |
L(2), B(2) |
1 |
B(1) |
3 |
||
R4 |
5 |
L(1), B(2) |
2 |
B(1) |
2 |
||
G7 |
0.500 |
R1 |
5 |
L(1), B(1) |
3 |
- |
2 |
R2 |
5 |
L(1) |
4 |
- |
1 |
||
R3 |
5 |
L(2), B(1) |
2 |
- |
3 |
||
R4 |
5 |
L(1), B(1) |
3 |
- |
2 |
R:
Replicate; h: hour; I: Immobilisation; N: Normal; B: Localized on
Bottom; L: Lethargy.
Alphabet outside and values inside the parentheses represent the
clinical symptom code and Number of Daphnids exhibiting that particular
symptom, respectively; -: Not applicable.
Table 2: TOC analysis during main study
Group |
Nominal Loading rates (mg/L) |
TOC During Exposure |
|||
Day 0 |
Day 1 (Aged) |
Day 1 (Fresh) |
Day 2 |
||
G1 |
0.0 |
- |
- |
- |
- |
G2 |
0.010 |
0.1128 |
0.1103 |
0.1205 |
0.1186 |
G3 |
0.021 |
0.1619 |
0.1423 |
0.1689 |
0.1681 |
G4 |
0.047 |
0.2525 |
0.2486 |
0.2612 |
0.2601 |
G5 |
0.103 |
0.3387 |
0.3296 |
0.3412 |
0.3386 |
G6 |
0.227 |
0.3747 |
0.372 |
0.3795 |
0.3743 |
G7 |
0.500 |
0.4005 |
0.3985 |
0.4028 |
0.4008 |
-: Not Applicable.
Description of key information
In the acute immobilisation test according to OECD guideline 202 the 48 hours acute median Effective Loading Rate (EL50) of Humectol LYS MOD on Daphnia magna is 0.0815 mg/L, the 95% confidence interval with lower limit of 0.0696 mg/L and upper limit of 0.0954 mg/L. No Observed Effect Loading Rate (NOELR) and Lowest Observed Effect Loading Rate (LOELR) over the 48 hours exposure period are 0.021 and 0.047 mg/L of Humectol LYS MOD respectively.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.082 mg/L
Additional information
The test item Humectol LYS MOD was evaluated
for acute toxicity on Daphnia magna according to OECD Guideline No. 202.
The acute toxicity study of Humectol LYS MOD on Daphnia magna was
performed to determine the 48 hours Effective concentration (EL50) and
to determine Lowest Observed Effect Nominal Loading Rate (LOELR) and No
Observed Effect Nominal Loading Rate (NOELR). Daphnids were exposed for
the test item by aqueous exposure (treatment) under semi-static
condition. Daphnids were observed at 24 and 48 hours for signs of
toxicity and immobilization.
Based on the results of a dose range finding study, main study was
conducted at the nominal loading rates of 0.010, 0.021, 0.047, 0.103,
0.227 and 0.500 mg/L of Humectol LYS MOD of test medium along with
control. No clinical signs of toxicity were observed in control and at
the nominal loading rate of 0.010 mg/L during the 48 hours observation
period. Clinical signs of daphnids localized on bottom of container and
lethargy was observed at the nominal loading rates of 0.021, 0.047,
0.103 and 0.227 mg/L during the 48 hours observation period. Clinical
signs of daphnids localized on bottom of container and lethargy was
observed at the nominal loading rates of 0.500 mg/L during the 24 hours
observation period. The immobilization percentages of 0, 10, 35, 60, 80
and 100% were observed at the nominal loading rates of 0.010, 0.021,
0.047, 0.103, 0.227 and 0.500 mg/L respectively during the 48 hours
observation period.
Based on the above results of the experiment and under experimental
conditions employed, it is concluded that the 48 hour acute median
effective concentration (EL50) of Humectol LYS MOD on Daphnia magna is
0.0815 mg/L; the 95 % confidence interval with lower limit of 0.0696
mg/L and upper limit of 0.0954 mg/L. NOELR and LOELR over the 48 hours
exposure period are 0.021 and 0.047 mg/L of Humectol LYS MOD,
respectively.
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