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Diss Factsheets
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EC number: 210-042-3 | CAS number: 603-47-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Final report on the safety assessment of Basic Violet 1, Basic Violet 3, and Basic Violet 4
- Author:
- C. Diamante et al.
- Year:
- 2 009
- Bibliographic source:
- International Journal of Toxicology, 28(Suppl 3) 193s-204s
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: no information on the method
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 4-[[4-(dimethylamino)phenyl][4-(methylimino)cyclohexa-2,5-dien-1-ylidene]methyl]-N,N-dimethylaniline monohydrochloride
- EC Number:
- 210-042-3
- EC Name:
- 4-[[4-(dimethylamino)phenyl][4-(methylimino)cyclohexa-2,5-dien-1-ylidene]methyl]-N,N-dimethylaniline monohydrochloride
- Cas Number:
- 603-47-4
- Molecular formula:
- C24H27N3.ClH
- IUPAC Name:
- 4-[[4-(dimethylamino)phenyl][4-(methylimino)cyclohexa-2,5-dien-1-ylidene]methyl]-N,N-dimethylaniline monohydrochloride
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- not specified
Test system
- Vehicle:
- not specified
- Controls:
- no
- Amount / concentration applied:
- 1% Gential Violet
- Duration of treatment / exposure:
- Administration 3 times a day but duration is not reported (at least 3 days)
- Observation period (in vivo):
- No information
- Number of animals or in vitro replicates:
- 2 rabbits
Results and discussion
In vivo
Results
- Irritation parameter:
- other: no information on the measured parameters
- Remarks on result:
- positive indication of irritation
- Irritant / corrosive response data:
- The two rabbits developed conjunctival congestion and discharge the day following the administration of 1% gentian violet. After 3 days of instillation, there was some necrosis of the conjunctiva.
Conjunctival biopsies had variable thinning of epithelial lining with total loss of goblet cells and subepithelial capillary congestion with neutrophilic infiltration.
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Conclusions:
- When 1% gentian violet was instilled in the eye of 2 rabbits 3 times a day (duration not reported), both animals developed conjunctival congestion and discharge the day following the administration of gentian violet. After 3 days of instillation, there was some necrosis of the conjunctiva. Conjunctival biopsies had variable thinning of epithelial lining with total loss of goblet cells and subepithelial capillary congestion with neutrophilic infiltration.
As no information regarding the reversibility of the effect nor on the measured parameters are mentioned, the classification can not be determined.
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