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EC number: 206-460-0 | CAS number: 345-35-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- July 2013
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- screening version (only conducted as preincubation modification, only one plate per dose and strain investigated, purity of test item not specified)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- α-chloro-2-fluorotoluene
- EC Number:
- 206-460-0
- EC Name:
- α-chloro-2-fluorotoluene
- Cas Number:
- 345-35-7
- Molecular formula:
- C7H6ClF
- IUPAC Name:
- 1-(chloromethyl)-2-fluorobenzene
- Test material form:
- liquid
Constituent 1
Method
- Target gene:
- Histidine gene locus
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced male rat liver S9 mix
- Test concentrations with justification for top dose:
- 0, 5, 10, 50, 100, 250, 500, 1000, 2000 µg/plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: sodium azide (TA 100 and TA 1535), 2-nitrofluoren (only TA 98), 4-nitro-1,2-phenylene diamine (only TA 1537), cumene hydroperoxide (only TA 102), 2-aminoanthracene (all strains)
- Remarks:
- The positive controls sodium azide, 2-nitrofluoren, 4-nitro-1,2-phenylene diamine and cumene hydroperoxide were only used without S9 mix; the positive control 2-aminoanthracene was only used with S9 mix.
- Details on test system and experimental conditions:
- METHOD: Preincubation test; only one plate per dose and strain investigated
- Evaluation criteria:
- The test substance is classified as mutagenic if there is a concentration-related increase over the range tested and/or an increase at one or more concentrations in the number of revertant colonies per plate in at least one strain with or without metabolic activation system. For TA 1535, TA 1537, TA 100 and TA 98 this increase should be about twice that of negative controls, whereas for TA 102 an increase of less than 2-fold may also be judged a positive result.
In the evaluation of the test results, the magnitude ofthe effects, their reproducibility, bacteriotoxic effects of the test substance, the historical control data obtained in the laboratory and scientific plausibility are taken into consideration.
Any positive test result should be evaluated for its biological relevance.
A test substance for which the results do not meet the above criteria is considered non-mutagenic in this test - Statistics:
- not specified
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA 98, TA 100, TA 102, TA 1535, TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- strain-specific bacteriotoxic effect at 500 µg per plate and above
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
The total colony counts of the 10-6 dilution of bacterial culture confirmed the viability and high cell density of the cultures of the individual strains.
The counts recorded on appropriate solvent control plates confirmed the characteristic spontaneous reversion rates of the tester strains. Furthermore, appropriate positive controls with known mutagens produced the expected numbers of revertant colonies.
Precipitates in the agar were not found.
Doses up to and including 250 μg per plate did not cause any bacteriotoxic effects. Total bacteria counts remained unchanged and no inhibition of growth was observed. At higher doses, the substance had a strain-specific bacteriotoxic effect. This range could nevertheless be used strain-specifically up to 2000 μg per plate for assessment purposes.
Evidence of mutagenic activity of 2-Fluorbenzylchloride was not seen. No biologically relevant increase in the mutant count, in comparison with the negative controls, was observed for any of the five Salmonella strains.
Due to these results 2-Fluorbenzylchloride has to be regarded as non-mutagenic in the Salmonella/microsome mutagenicity test.
Applicant's summary and conclusion
- Conclusions:
- negative
- Executive summary:
2-Fluorbenzylchloride was initially screened with one plate per dose using the Salmonella/microsome preincubation test for point mutagenic effects in doses of up to and including 2000 µg per plate on the five Salmonella typhimurium strains TA 98, TA 100, TA 102, TA 1535 and TA 1537 (similar to OECD TG 471).
Doses up to and including 250 μg per plate did not cause any bacteriotoxic effects. Total bacteria counts remained unchanged and no inhibition of growth was observed. At higher doses, the substance had a strain-specific bacteriotoxic effect. This range could nevertheless be used strain-specifically up to 2000 μg per plate for assessment purposes.
Evidence of mutagenic activity of 2-Fluorbenzylchloride was not seen. No biologically relevant increase in the mutant count, in comparison with the negative controls, was observed for any of the five Salmonella strains.
Therefore, 2-Fluorbenzylchloride was considered to be non-mutagenic without and with S9 mix in the preincubation modification of the Salmonella/microsome test.
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