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Diss Factsheets
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EC number: 208-142-7 | CAS number: 512-42-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Sodium methyl sulphate
- EC Number:
- 208-142-7
- EC Name:
- Sodium methyl sulphate
- Cas Number:
- 512-42-5
- Molecular formula:
- CH4O4S.Na
- IUPAC Name:
- sodium methyl sulphate
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: KIRSCHAZ2-00182
- Expiration date of the lot/batch: 2018-07-07
- Water solubility: 925 g/L ± 9 g/L at T = 20.0 °C ± 0.5 °C
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature, protect from light, in the tightly closed original
container
- Stability under test conditions: stable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: One limit concentration of 120 mg/L
- Sample storage conditions before analysis: All original samples were stored at room temperature. Prepared samples were stored at room temperature in an autosampler until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
120 mg test item/L was freshly prepared with dilution water. The dispersion was agitated until the stock solution was clear. Before use the completeness of dissolution was checked
via Laser beam (Tyndall effect), which was negative.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriel/a subcapitata HINDAK, SAG 61.81
- Source (laboratory, culture collection): Sammlung von Algenkulturen (SAG) Pflanzenphysiologisches Institut der Universität Göttingen, D-37073 Göttingen
- Cultivation at test facility: Fresh stocks are prepared every month on Z-Agar. Light intensity amounted to 2590 - 5180 lux for 24 hours per day.
- Age of inoculum (at test initiation): A three day old preculture, prepared in dilution water, was used as inoculum
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.8 -23.0 °C
- pH:
- 7.86-8.50
- Nominal and measured concentrations:
- Nominal: 120 mg/L, measured: 130-142 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Sterile Erlenmeyer flasks,
- Type (delete if not applicable): sealed with cotton wool plugs
- Material, size, headspace, fill volume: 250 mL
- Initial cells density: 6839 cells/mL
- Control end cells density: 1926568 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the guideline
OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Photoperiod: 24 hours/day light
- Light intensity and quality: 4630 to 5602 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]
- Chlorophyll measurement: Cell density was measured daily via Chlorophyll a-fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as a background signal. No self-fluorescence was found in the
definitive test at the concentration of 120 mg/L.
- Other: Microscopic evaluation of the cells at the start and end of the exposure was carried out. The cells were checked for unusual cell shapes, colour differences, differences in chloroplast morphology,
flocculation, adherence of algae to test containers and aggregation of algae cells.
TEST CONCENTRATIONS
-One limit concentration of 120 mg/L was tested. The concentration level is based on the results of a preliminary range finding test (non-GLP). - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 120 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- - Results with reference substance:
- ErC50: 0.435 mg/L
:
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study, no inhibition of the growth rate but a slight effect on yield (16% inhibition in comparison with the control) of the freshwater green alga Pseudokirchneriella subcapitata was found after 72 hours exposure to the test item Sodium methyl sulfate. The nominal EC50-values for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were > 120 mg test item/L, respectively. The nominal NOEC-value for inhibition of growth rate was 120 mg test item/L. The NOEC-value for yield was < 120 mg test item/L. All effect values given are based on nominal test item concentration of Sodium methyl sulfate.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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