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Diss Factsheets
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EC number: 906-521-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 - 30 Nov 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Niedersächsisches Umweltministerium, Hannover, Germany
Test material
- Reference substance name:
- Reaction mass of 2-hydroxyisopropyl 2-hydroxypropyl bicarbamate and 2-hydroxypropyl carbazate and propylene carbonate
- EC Number:
- 906-521-8
- Cas Number:
- 85391-60-2
- Molecular formula:
- C8H16N2O6
- IUPAC Name:
- Reaction mass of 2-hydroxyisopropyl 2-hydroxypropyl bicarbamate and 2-hydroxypropyl carbazate and propylene carbonate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 100 mg/L
- Sampling method: Samples were taken from the test solutions and an additional replicate without test organisms (duplicate samples).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 200 mg test substance was applied to 2 L of test medium. The solution was shaken with 20 rpm for 24 h. Thereafter, the dispersion was centrifuged (20 min, 3000 rpm) to remove undissolved particles.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: Aquarium am Aegi, Hannover, Germany
- Maintenance of the brood fish: Holding was performed at 23 ± 2 °C and diffuse light. Water was changed at least once per week. No disease treatments were administered throughout holding and testing.
- Length at study initiation: 2.67 cm
- Weight at study initiation: 0.2 g
ACCLIMATION
- Acclimation period: at least 12 d (mortality < 5% within the last 7 d of acclimatisation)
- Acclimation conditions (same as test or not): same as test
- Type and amount of food during acclimation: Trouvit 40 / 2, Milkivit; Bürgheim, Germany
- Feeding frequency during acclimation: Three times a week (4% of fish body weight per day); Fish were not fed until 24 h before test start.
FEEDING DURING TEST
- None
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 76 mg/L as CaCO3
- Test temperature:
- 21.0 - 22.5 °C
- pH:
- 7.04 - 7.53
- Dissolved oxygen:
- 100% oxygen saturation
- Nominal and measured concentrations:
- nominal: control, 100 mg/L
measured DOC: please refer to "Any other information on results incl. tables" - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria with test volume of 2 L per vessel.
- Type (delete if not applicable): loosely covered by glass tops
- Aeration: gentle aeration was provided
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 g fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water of local origin; water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine
- Total hardness: 40 - 180 mg CaCO3/L
- pH: 6.0 - 8.0
- Culture medium different from test medium: same as test
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: natural photoperiod
- Light intensity: 0.1 - 10 µmol/m²*s
EFFECT PARAMETERS MEASURED
- Mortality was recorded after 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable, limit test
- Range finding study
- Test concentrations: One limit Water Accommodated Fraction (WAF) was tested incl. a control
- Results used to determine the conditions for the definitive study: No mortality was recorded. Thus, a limit test is justified. - Reference substance (positive control):
- not required
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0%
- Reported statistics and error estimates:
- Since no effects were recorded, no statistical analysis was necessary.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table 1: Results from DOC analysis
Nominal loading [mg/L]
DOC [mg/L]
Parallel determination
Mean
0 h
96 h
0 h
96 h
100
--
--
46.5
47.0
--
46.8
100*
43.3
43.5
42.8
43.1
43.4
43.0
Control
--
--
6.4
6.5
--
6.5
Control*
1.6
1.6
1.7
1.8
1.6
1.8
* test loading without test organisms
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Mortality in control: 0%; O2 saturation: 100%
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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