Essential oil of Matricaria recutita (Asteraceae) obtained from flower tops by steam distillation of European origin

Melting point / freezing point

Link to relevant study record(s)

Reference

Endpoint:

melting point/freezing point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 23 to 24 November 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

The study was conducted according to an internationally recognised method, and under GLP. The substance is considered to be adequately characterised. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 102 (Melting point / Melting Range)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.1 (Melting / Freezing Temperature)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes (incl. QA statement)

Remarks:

GLP compliance programme (inspected on 20-21 June 2017 / signed on 4 September 2017)

Type of method:

differential scanning calorimetry

Key result

Melting / freezing pt.:

< -20 °C

Decomposition:

no

Results

Melting point / melting range : Differential scanning calorimetry

Two DSC measurements in aluminium crucibles with a hole with addition of glass pearls were performed. In each case the test item was cooled down to -50 °C with a cooling rate of 1 K/min, kept at ‑50 °C for 30 min and afterwards the test item was heated up to 500 °C with a heating rate of 10 K/min. No effect was observed during the cooling phase and keeping the test item at -50 °C. During the heating phase an exothermal effect was observed starting at 180 °C, directly followed by an endothermal effect.

Graphical presentations of the DSC runs are shown on page 7 of the experimental report. No event was observed when the test item was cooled down to -50°C.

Under consideration of the result from the DSC measurement it was concluded that the test item had no freezing/melting point until -20 °C.

Conclusions:

The freezing point of test item was stated as < - 20 °C (< 253.15 K).

Executive summary:

The freezing point of the test item was determined under GLP according to EU Method A.1 and OECD 102.

 

In a DSC test, no effect was observed during the cooling phase and keeping the test item at -50 °C. According to Regulation (EC) No 1907/2006 (REACH) annex VII, column 2 of section 7.2, the determination of the melting / freezing point does not need to be conducted below a lower limit of - 20 °C. Therefore, no additional test was conducted and the freezing point is stated as < - 20 °C (< 253.15 K).

Description of key information

The freezing point is stated as: < - 20 °C (< 253.15 K).

Additional information

A fully reliable experimental study, conducted according to a recognized OECD/EC method and under GLP, is available. It is considered as a key study.

In a DSC test, no effect was observed during the cooling phase and keeping the test item at -50 °C. According to Regulation (EC) No 1907/2006 (REACH) annex VII, column 2 of section 7.2, the determination of the melting / freezing point does not need to be conducted below a lower limit of - 20 °C.