Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 916-461-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From May 30 to June 18, 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Details on test solutions:
- Test item was directly dispersed in water.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout.
- Source: Donnington Fish Farm, Upper Swell, Gloucester U.K.
- Length at study initiation: 4.9 ± 0.2 cm
- Weight at study initiation: 1.49 ± 0.20 g
ACCLIMATION
- Health during acclimation: no deaths during the 7 days prior to the study start. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 14 °C
- Nominal and measured concentrations:
- Nominal 10 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 20 litres
- Aeration: yes.
- Renewal rate of test solution: daily.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control : 1 replicate.
- Biomass loading rate: 0.75 g bw/litre
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: dechlorinated tap water (by passage through an activated carbon filter).
- Hardness: approximately 100 mg/l as CaCO3.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1.0, 10 and 100 mg/l
- No. of organisms per concentratyion: 3 fish.
- Results used to determine the conditions for the definitive study: initially a limit test was carried out at 100 mg/l. During the experiment progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the test concentration of 100 mg/l. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration. - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 10 - 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
DEFINITIVE TEST
Concentration (mg/l) Cumulative mortality (initial population: 3) Control 24 hrs 48 hrs 72 hrs 96 hrs 10 0 0 0 0 10 0 0 0 0 DOSE RANGE FINDING
Concentration (mg/l) Cumulative mortality (initial population: 3) Control 24 hrs 48 hrs 72 hrs 96 hrs 1 0 0 0 0 10 0 0 0 0 100 0 0 0 0* *initially a limit test was carried out at 100 mg/l. During the experiment, progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the test concentration of 100 mg/l. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration.
- Conclusions:
- LC50 (96h): 10 - 100 mg/l (nominal)
- Executive summary:
The acute lethal toxicity of the test substance to rainbow trout was investigated under semi-static exposure conditions over a period of 96 hours, following the OECD guideline 203. Initially a limit test was carried out at 100 mg/l. During the experiment, progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions, testing the concentrations of 1, 10 and 100 mg/l, adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the highest tested concentration. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration.
No mortality was also observed in the blank control and in the test vessels.
The LC50 was indicated to be between 10 and 100 mg/l.
Conclusion
LC50 (96h): 10 - 100 mg/l (nominal)
Reference
Description of key information
Harmful for short-term exposure to fish; LC50 (96h) between 10 - 100 mg/l (nominal)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 10 mg/L
Additional information
The acute lethal toxicity of the test substance to rainbow trout was investigated under semi-static exposure conditions over a period of 96 hours, following the OECD guideline 203. Initially a limit test was carried out at 100 mg/l. During the experiment, progressive mortalities occurred due to the semi-static conditions employed as opposed to the static test conditions under which the range-finding test was carried out. On repeating the range-finding under semi-static conditions, testing the concentrations of 1, 10 and 100 mg/l, adverse reactions to exposure (i.e. 3/3 fish swimming at the bottom of the test vessel) were observed at the highest tested concentration. Therefore, the definitive study was repeated at 10 mg/l to determine a true no effect concentration. No mortality was also observed in the blank control and in the test vessels.
The LC50 was indicated to be between 10 and 100 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.