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EC number: 231-601-8 | CAS number: 7647-18-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study was generated according to valid testing guideline: OECD guideline 471
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Diantimony trioxide
- EC Number:
- 215-175-0
- EC Name:
- Diantimony trioxide
- Cas Number:
- 1309-64-4
- Molecular formula:
- Sb2O3
- IUPAC Name:
- dioxodistiboxane
- Test material form:
- not specified
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Species / strain / cell type:
- E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- The doses tested were 100, 200, 500, 1000, 2500 and 5000 µg/plate.
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- further positive control substances: ICR, daunorubicin, ethylnitrosoguanidine, 2-aminoanthracene, mitomycin c
- Details on test system and experimental conditions:
- METHOD OF APPLICATION:
- in agar (plate incorporation) and preincubation
- For the plate incorporation protocol, 0.5 ml of S9-mix (or buffer), 0.1 ml of bacteria culture, 0.1 ml of test compound and 2.0 ml top agar were mixed and poured onto agar plates. These were incubated at 37°C for 3 days before counting on an automatic colony counter.
- The preincubation protocol (conducted for the presence of S9-mix) included 60 minutes incubation at 37°C on an orbital shaker before the addition of top agar.
DURATION
- Preincubation period: 60 minutes
OTHER:
- The S9-mix was prepared from male Sprague-Dawley rates dosed daily by oral gavage for 3 days with a combined phenobarbital (80 mg/kg bw) and ß-naphthoflavone (100 mg/kg) corn oil solution. The cofactor was a solution of Na2HPO4, KCl, glucose-6-phosphate, NADP (Na salt) and MGCl2, pH 7.4.
- no other details on test system stated - Evaluation criteria:
- Increase of the numers of bacteria colonies.
- Statistics:
- No statistics were performed to evaluate the result.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES:
- Antimony trioxide induced no increases in revertant numbers in four S. typhimurium and two E. coli tester strains in two independent assays, including both plate incorporation and preincubation protocols in the presence of S9-mix, at doses up to 5000 µg/plate. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Assessment of antimony trioxide in the Salmonella/microsome assay
Strain |
Concentration (µg/plate) |
Mean revertants per plate |
|||
. |
. |
- S9-mix Phase1 |
+ S9-mix Phase 1 |
- S9-mix Phase2 |
+ S9-mix Phase 2 (preincubation) |
TA 1535 |
5000 |
11.7 |
11.0 |
6.7 |
14.7 |
2500 |
14.3 |
14.3 |
6.3 |
15.0 |
|
1000 |
10.3 |
16.7 |
7.7 |
12.3 |
|
500 |
12.3 |
16.0 |
9.0 |
13.0 |
|
200 |
10.0 |
15.7 |
11.0 |
13.0 |
|
100 |
14.0 |
17.7 |
6.7 |
12.7 |
|
0 |
10.8 (1194)a |
15.0 (203)e |
9.0 (793)a |
14.4 (106)f |
|
TA 1537 |
5000 |
4.0 |
5.3 |
4.3 |
1.7 |
2500 |
5.3 |
4.3 |
2.7 |
3.0 |
|
1000 |
4.3 |
4.7 |
2.7 |
3.3 |
|
500 |
3.7 |
4.7 |
3.0 |
3.3 |
|
200 |
3.7 |
4.0 |
3.7 |
5.0 |
|
100 |
3.7 |
3.7 |
3.3 |
3.7 |
|
0 |
3.0 (64)b |
3.6 (82)e |
3.4 (45)b |
4.0 (29)f |
|
TA 98 |
5000 |
19.3 |
12.3 |
17.3 |
16.0 |
2500 |
19.7 |
21.0 |
17.3 |
22.0 |
|
1000 |
23.7 |
26.7 |
18.0 |
19.7 |
|
500 |
20.3 |
21.7 |
16.7 |
23.0 |
|
200 |
22.3 |
21.7 |
19.3 |
23.0 |
|
100 |
19.3 |
19.7 |
17.3 |
23.0 |
|
0 |
20.8 (108)c |
23.6 (673)f |
17.4 (395)c |
21.2 (288)f |
|
TA 100 |
5000 |
85.7 |
108.7 |
97.7 |
139.0 |
2500 |
82.0 |
124.0 |
109.7 |
123.0 |
|
1000 |
92.0 |
132.3 |
95.3 |
127.0 |
|
500 |
81.3 |
120.7 |
115.3 |
127.7 |
|
200 |
83.0 |
124.7 |
109.3 |
135.0 |
|
100 |
78.0 |
118.7 |
125.3 |
132.7 |
|
0 |
82.4 (788)a |
92.6 (802)f |
117.2 (1681)a |
119.8 (899)f |
|
WP2P |
5000 |
34.3 |
51.7 |
23.3 |
56.3 |
2500 |
35.0 |
42.7 |
23.7 |
52.3 |
|
1000 |
34.0 |
52.3 |
22.3 |
49.3 |
|
500 |
38.7 |
45.7 |
24.7 |
52.7 |
|
200 |
40.7 |
50.7 |
22.3 |
50.3 |
|
100 |
34.7 |
40.3 |
29.3 |
56.3 |
|
0 |
35.6 (210)h |
47.6 (176)g |
30.6 (159)h |
50.4 (182)g |
|
WP2PuvrA |
5000 |
110.7 |
163.7 |
89.7 |
156.7 |
2500 |
112.0 |
165.0 |
89.7 |
149.0 |
|
1000 |
117.0 |
150.3 |
99.0 |
157.0 |
|
500 |
105.7 |
157.3 |
93.0 |
132.0 |
|
200 |
130.3 |
168.3 |
89.0 |
144.7 |
|
100 |
106.0 |
515.7 |
96.3 |
147.3 |
|
0 |
97.8 (1681)d |
139.8 (1009)e |
89.6 (2962)d |
128.6 (1335)e |
All mean revertants per plate values are the mean of three plates, except solvent controls (0µg/ml) which are the mean of five plates.
Positive controls (µg/plate).
a Sodium azide (2.0); b ICR 191 (2.0); cDaunorubicin (1.0); d Ethylnitronitrosoguanidine (1.0); e2-Aminoanthracene (2.0); f 2-Aminoanthracene (1.0); g2-Aminoanthracene (20); h Mitomycin C (1.0)
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Diantimony trioxide did not induce reverse mutations in bacteria in this study under the conditions described.
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